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Very thin membranes, such as the rat iris or mesentery, do not have to be sectioned for microscopic studies but may simply be spread on glass, dried, and then exposed to gaseous formaldehyde for subsequent study with a fluorescence microscope.
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The F-H method allowed, for the first time, the examiner to watch these monoamines light up in the microscope and to precisely determine in which cells they were present, and thereby understanding their functions. The method was developed by
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In 2012, the
Faculty of Medicine at the University of Lund arranged a symposium “From Nerve to Pills” celebrating the 50th anniversary of the initial publication of the F-H method.
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The initial publication, written already in 1961, described a wide-ranging examination of nerves supplying a large number of organs in the body. This work validated the concept of
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Falck, B.; Hillarp, N. A.; Thieme, G.; Torp, A. (1982). "Fluorescence of catechol amines and related compounds condensed with formaldehyde".
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Falck, B. (1962). "Observations on the possibilities of the cellular localization of monoamines by a fluorescence method".
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35:) is a technique that makes it possible to demonstrate and study, with unique precision and susceptibility, certain
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FALCK B; TORP A (1962). "New evidence for the localization of noradrenalin in the adrenergic nerve terminals".
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The publication on the chemical background was later named among "The 200 Most-Cited Papers of All Time".
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The method is based on the important and decisive discovery that these compounds are able to react with
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and for the first time comprehend that these substances act as signal substances, i.e. transmitters.
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