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in eukaryotic cells. Living cells will actively convert the non-fluorescent FDA into the green fluorescent compound fluorescein, a sign of viability; while nucleus of membrane-compromised cells will fluoresce red, a sign of cell death. Currently FDA/PI staining is the standard assessment of human
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FDA hydrolysis is often used to measure activity in soil and compost samples; however, it may not give an accurate reading if microbes with lower activity phases, such as esterases, cleave the
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of microbes in a sample. A bright yellow-green glow is produced and is strongest when enzymatic activity is greatest. This can be quantified using a
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217:"Fluorescein diacetate hydrolysis as a measure of microbial activity in aquatic systems: Application to activated sludges"
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United States
Department of Agriculture (USDA): Assay for Fluorescein Diacetate Hydrolytic Activity for soil samples
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may be added for live cell viability testing. FDA stain must be kept in the dark at 4°C or it will spoil.
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pancreatic islet viability with suitability for transplantation when viability score is above 70%.
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http://www.eeescience.utoledo.edu/Faculty/Sigler/Von_Sigler/LEPR_Protocols_files/FDA%20assay.pdf
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FDA stock solution is prepared by dissolving 5 mg of fluorescein diacetate in 1 ml
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Fluorescein
Diacetate: A Potential Biological Indicator for Arid Soils
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Fontvieille, D.A.; Outaguerouine, A.; Thevenot, D.R. (1992).
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