98:) from a single sample, or combined set of samples, or when long-term storage of part of the sample is desired. Cryohomogenization can be carried out using a supercooled mortar and pestle (classic approach), or the tissue can be homogenized by crushing it into a fine powder inside a clean plastic bag resting against a supercooled solid metal block (more recently developed and more efficient technique).
51:, or an intentional process in research: A homogenized sample is equal in composition throughout, so that removing a fraction does not alter the overall molecular make-up of the sample remaining, and is identical to the fraction removed. Induced homogenization in biology is often followed by molecular extraction and various analytical techniques, including
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Chang, Ta-Yuan; Limanek, James S.; Chang, Catherine C.Y. (1 September 1981). "A simple and efficient procedure for the rapid homogenization of cultured animal cells grown in monolayer".
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von
Ziegler, Lukas M.; Saab, Bechara J.; Mansuy, Isabelle M. (March 27, 2013). "A simple and fast method for tissue cryohomogenization enabling multifarious molecular extraction".
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can be performed under "dry" conditions, and is often the method of choice whenever it is desirable to collect several distinct molecular classes (e.g. both
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Wuytack, Elke Y; Diels, Ann M.J; Michiels, Chris W (1 August 2002). "Bacterial inactivation by high-pressure homogenisation and high hydrostatic pressure".
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Schmidt SD, Nixon RA, Mathews PM (2012). "Tissue
Processing Prior to Analysis of Alzheimer's Disease Associated Proteins and Metabolites, Including Aβ".
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Singh, Kaustub; Gupta, Ankur; Buchner, Abel John; Ibis, Fatma; Pronk, Joachim W.; Tam, Daniel; Eral, Huseyin Burak (1 July 2019).
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is a technique suitable for soft mammalian tissues, while lysis of mammalian cells has also been demonstrated via centrifugation.
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47:, is a process whereby different fractions of a biological sample become equal in composition. It can be a disease sign in
105:, since these cells are exceptionally resistant to lysis, and may be combined with high-temperature sterilization.
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Homogenization of tissue in solution is often performed simultaneously with cell
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24:, pathologic homogenization is seen as a loss of variations, such as of
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132:. Methods in Molecular Biology. Vol. 849. pp. 493–506.
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High-pressure homogenization is used to isolate the contents of
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71:) can be kept at temperatures slightly above zero to prevent
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249:International Journal of Food Microbiology
288:Journal of Colloid and Interface Science
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86:If freezing the tissue is possible,
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206:Journal of Neuroscience Methods
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261:10.1016/S0168-1605(02)00054-5
183:10.1016/0003-2697(81)90360-2
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171:Analytical Biochemistry
103:Gram-positive bacteria
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346:Laboratory techniques
109:Dounce homogenization
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79:solution to prevent
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55:and western blot.
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294:: 127–135.
32:(pictured).
330:Categories
115:References
73:autolysis
316:96448552
308:30952074
269:12160080
234:34417825
226:23541735
156:22528111
83:damage.
77:isotonic
26:collagen
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81:osmotic
59:Methods
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312:S2CID
230:S2CID
65:lysis
53:ELISA
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265:PMID
222:PMID
187:PMID
152:PMID
142:ISBN
94:and
296:doi
292:547
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96:RNA
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