343:
Wildgruber, Robert; Reil, Gerold; Drews, Oliver; Parlar, Harun; Görg, Angelika (2002-06-01). "Web-based two-dimensional database of
Saccharomyces cerevisiae proteins using immobilized pH gradients from pH 6 to pH 12 and matrix-assisted laser desorption/ionization-time of flight mass spectrometry".
75:
defined by its pK value. Immobilized pH gradients (IPG) are made by mixing two kinds of acrylamide mixture, one with
Immobiline having acidic buffering property and other with basic buffering property. The concentrations of the buffers in the two solutions define the range and shape of the pH
17:
211:
Corbett, J. M.; Dunn, M. J.; Posch, A.; Görg, A. (1994-09-01). "Positional reproducibility of protein spots in two-dimensional polyacrylamide gel electrophoresis using immobilised pH gradient isoelectric focusing in the first dimension: an interlaboratory comparison".
255:
Blomberg, A.; Blomberg, L.; Norbeck, J.; Fey, S. J.; Larsen, P. M.; Larsen, M.; Roepstorff, P.; Degand, H.; Boutry, M. (1995-10-01). "Interlaboratory reproducibility of yeast protein patterns analyzed by immobilized pH gradient two-dimensional gel electrophoresis".
299:
Wildgruber, R.; Harder, A.; Obermaier, C.; Boguth, G.; Weiss, W.; Fey, S. J.; Larsen, P. M.; Görg, A. (2000-07-01). "Towards higher resolution: two-dimensional electrophoresis of
Saccharomyces cerevisiae proteins using overlapping narrow immobilized pH gradients".
80:
monomers to form a polyacrylamide gel. These polymerised gels are backed with plastic based backing that allow ease in handling and improve IPG's performance. The gel is then washed to remove catalysts and unpolymerized
387:
Görg, A.; Postel, W.; Friedrich, C.; Kuick, R.; Strahler, J. R.; Hanash, S. M. (1991-09-01). "Temperature-dependent spot positional variability in two-dimensional polypeptide patterns".
59:
gradient, which result in completely stable gradients except the most alkaline (>12) pH values. The immobilized pH gradient is obtained by the continuous change in the ratio of
76:
gradient produced. Both solutions contain acrylamide monomers and catalysts. During polymerization, the acrylamide portion of the buffers co polymerize with the acrylamide and
93:. Other advantages are increased resolution, reproducible separation of alkaline proteins and increased loading capacity (up to 10 mg).
444:
313:
168:
Görg, Angelika; Weiss, Walter; Dunn, Michael J. (2004-12-01). "Current two-dimensional electrophoresis technology for proteomics".
116:
Görg, Angelika; Weiss, Walter; Dunn, Michael J. (2004-12-01). "Current two-dimensional electrophoresis technology for proteomics".
96:
This alternative method eliminates the problems of gradient instability and poor sample loading capacity associated with carrier
357:
158:
Barnes et al., Electrophoresis in
Practice: A Guide to theory and Practice. Page 44. VCH Publishers Inc, 1993 (USA)
32:
429:
Wilkins et al., Proteome
Research: New Frontiers in Functional Genomics. Page 14. Springer, 1997 (Germany)
86:
90:
16:
412:
404:
369:
361:
325:
317:
281:
273:
237:
229:
193:
185:
141:
133:
396:
353:
309:
265:
221:
177:
125:
72:
52:
438:
77:
85:, which interfere with isoelectric separation. IPG increased reproducibility of
48:
408:
365:
321:
277:
269:
233:
225:
189:
137:
400:
97:
28:
373:
329:
197:
181:
145:
129:
416:
314:
10.1002/1522-2683(20000701)21:13<2610::AID-ELPS2610>3.0.CO;2-H
285:
241:
82:
358:
10.1002/1615-9861(200206)2:6<727::AID-PROT727>3.0.CO;2-2
68:
44:
56:
100:
pH gradient. Commercial precast IPG gels are available.
8:
15:
108:
7:
14:
1:
461:
270:10.1002/elps.11501601320
226:10.1002/elps.11501501182
401:10.1002/elps.1150120910
37:Immobilized pH gradient
182:10.1002/pmic.200401031
130:10.1002/pmic.200401031
24:
20:General structure of
19:
87:isoelectric focusing
445:Acid–base chemistry
91:gel electrophoresis
33:acid–base reactions
220:(8–9): 1205–1211.
25:
308:(13): 2610–2616.
264:(10): 1935–1945.
176:(12): 3665–3685.
124:(12): 3665–3685.
452:
430:
427:
421:
420:
384:
378:
377:
340:
334:
333:
296:
290:
289:
252:
246:
245:
208:
202:
201:
165:
159:
156:
150:
149:
113:
460:
459:
455:
454:
453:
451:
450:
449:
435:
434:
433:
428:
424:
389:Electrophoresis
386:
385:
381:
342:
341:
337:
302:Electrophoresis
298:
297:
293:
258:Electrophoresis
254:
253:
249:
214:Electrophoresis
210:
209:
205:
167:
166:
162:
157:
153:
115:
114:
110:
106:
12:
11:
5:
458:
456:
448:
447:
437:
436:
432:
431:
422:
395:(9): 653–658.
379:
352:(6): 727–732.
335:
291:
247:
203:
160:
151:
107:
105:
102:
53:co-polymerized
13:
10:
9:
6:
4:
3:
2:
457:
446:
443:
442:
440:
426:
423:
418:
414:
410:
406:
402:
398:
394:
390:
383:
380:
375:
371:
367:
363:
359:
355:
351:
347:
339:
336:
331:
327:
323:
319:
315:
311:
307:
303:
295:
292:
287:
283:
279:
275:
271:
267:
263:
259:
251:
248:
243:
239:
235:
231:
227:
223:
219:
215:
207:
204:
199:
195:
191:
187:
183:
179:
175:
171:
164:
161:
155:
152:
147:
143:
139:
135:
131:
127:
123:
119:
112:
109:
103:
101:
99:
94:
92:
88:
84:
79:
78:bisacrylamide
74:
70:
66:
62:
58:
54:
50:
46:
42:
38:
34:
30:
23:
18:
425:
392:
388:
382:
349:
345:
338:
305:
301:
294:
261:
257:
250:
217:
213:
206:
173:
169:
163:
154:
121:
117:
111:
95:
64:
60:
40:
36:
26:
21:
61:Immobilines
51:gel matrix
22:Immobiline.
346:Proteomics
170:Proteomics
118:Proteomics
104:References
67:is a weak
65:Immobiline
49:acrylamide
409:0173-0835
366:1615-9853
322:0173-0835
278:0173-0835
234:0173-0835
190:1615-9853
138:1615-9853
98:ampholyte
55:with the
29:chemistry
439:Category
374:12112855
330:10949137
198:15543535
146:15543535
83:monomers
47:are the
417:1752246
286:8586069
242:7532129
89:and 2D-
27:Within
415:
407:
372:
364:
328:
320:
284:
276:
240:
232:
196:
188:
144:
136:
63:. An
413:PMID
405:ISSN
370:PMID
362:ISSN
326:PMID
318:ISSN
282:PMID
274:ISSN
238:PMID
230:ISSN
194:PMID
186:ISSN
142:PMID
134:ISSN
73:base
69:acid
45:gels
31:for
397:doi
354:doi
310:doi
266:doi
222:doi
178:doi
126:doi
71:or
41:IPG
441::
411:.
403:.
393:12
391:.
368:.
360:.
348:.
324:.
316:.
306:21
304:.
280:.
272:.
262:16
260:.
236:.
228:.
218:15
216:.
192:.
184:.
172:.
140:.
132:.
120:.
57:pH
43:)
35:,
419:.
399::
376:.
356::
350:2
332:.
312::
288:.
268::
244:.
224::
200:.
180::
174:4
148:.
128::
122:4
39:(
Text is available under the Creative Commons Attribution-ShareAlike License. Additional terms may apply.
