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20:
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Conventional (up to 10 different oligonucleotides) existing molecule library serves as a starting point for the subsequent SELEX process. Selection, separation, and amplification using the mirror image of the target molecule is performed.
228:
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Wlotzka, Britta; Leva, Susanne; Eschgfäller, Bernd; Burmeister, Jens; Kleinjung, Frank; Kaduk, Christine; Muhn, Peter; Hess-Stumpp, Holger; Klussmann, Sven (June 2002).
326:-RNA aptamers has recently been demonstrated in diabetic nephropathy patients. They can also be used as diagnostic agents.
251:. In the case of peptides and small proteins that are produced synthetically, an enantiomer is made using synthetic
422:"In vivo properties of an anti-GnRH Spiegelmer: an example of an oligonucleotide-based therapeutic substance class"
484:
Klussmann S, Nolte A, Bald R, Erdmann VA, Fürste JP (September 1996). "Mirror-image RNA that binds D-adenosine".
236:
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176:-RNA aptamers to their target molecules often lies in the pico to nanomolar range and is thus comparable to
133:
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322:. They are currently in preclinical or clinical development. Proof-of-concept for an anti-CCL2/MCP-1
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194:-RNA aptamers have high stability in blood serum, since they are less susceptible to be cleaved
93:-RNA aptamers are considered potential drugs and are currently being tested in clinical trials.
259:. If the target is a larger protein molecule, beyond synthetic abilities, the enantiomer of an
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381:"Turning mirror-image oligonucleotides into drugs: the evolution of Spiegelmer therapeutics"
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Helmling, S.H.; Eulberg, D.E.; Maasch, C.M.; Buchner, K.B.; Klussmann, S.K. (July 2003).
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The sequence of the oligonucleotide selected using SELEX is determined with the help of
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239:(PCR), used in SELEX. Therefore, the selection is done with mirrored target molecules.
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284:. This information is used for the synthesis of the oligonucleotide's enantiomer, the
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in a short time due to their low molar mass (which is below the renal threshold).
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345:"RNA-Spiegelmers: a new substance class to efficiently inhibit peptide hormones"
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Proceedings of the
National Academy of Sciences of the United States of America
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120:-ribose, are the enantiomers of natural oligonucleotides, which are made with
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235:-nucleic acids are not amenable to enzymatic methods, such as
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172:, and substances of low molecular weight. The affinity of
85:-nucleotides, they are highly resistant to degradation by
229:
systematic evolution of ligands by exponential enrichment
208:-RNA aptamers modified with a higher molar mass, such as
215:-RNA aptamers, show a prolonged plasma half-life.
247:The first step is the production of the target's
164:-RNA aptamers are able to bind molecules such as
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227:-RNA aptamers are not directly made using
124:-ribose. Nucleic acid aptamers, including
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303:-RNA aptamers have been obtained for the
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379:Vater A, Klussmann S (January 2015).
198:by enzymes. They are excreted by the
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186:-RNA aptamers themselves have low
14:
243:Reflection of the target molecule
190:. In contrast to other aptamers,
349:European Journal of Biochemistry
362:10.1111/j.1474-3833.2004.4119.x
1:
73:of natural oligonucleotides.
398:10.1016/j.drudis.2014.09.004
314:, the complement components
77:-RNA aptamers are a form of
116:-RNA aptamers, built using
65:units. It is an artificial
23:3-D structural model of an
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156:Biological characteristics
57:-like molecule built from
237:polymerase chain reaction
38:-ribonucleic acid aptamer
276:Sequencing and synthesis
128:-RNA aptamers, contain
223:Unlike other aptamers,
134:guanosine monophosphate
130:adenosine monophosphate
447:10.1073/pnas.132067399
138:cytidine monophosphate
110:
28:
160:Like other aptamers,
142:uridine monophosphate
109:
27:-RNA aptamer fragment
22:
498:10.1038/nbt0996-1112
385:Drug Discovery Today
288:-RNA aptamer, using
152:and a ribose sugar.
438:2002PNAS...99.8898W
102:Chemical properties
111:
69:named for being a
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16:RNA-like molecule
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432:(13): 8898–902.
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146:phosphate group
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81:. Due to their
67:oligonucleotide
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391:(1): 147–155.
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292:-nucleotides.
282:DNA sequencing
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196:hydrolytically
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492:(9): 1112–5.
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231:(SELEX), as
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188:antigenicity
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71:mirror image
50:
47:-RNA aptamer
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42:
37:
33:
30:
257:amino acids
528:Categories
330:References
305:chemokines
249:enantiomer
219:Production
178:antibodies
150:nucleobase
51:Spiegelmer
210:PEGylated
87:nucleases
539:Peptides
514:41395593
466:12070349
407:25236655
170:proteins
166:peptides
97:Features
79:aptamers
53:) is an
506:9631061
434:Bibcode
320:ghrelin
261:epitope
200:kidneys
512:
504:
464:
457:124395
454:
405:
355:(S1).
312:CXCL12
63:ribose
510:S2CID
267:SELEX
502:PMID
462:PMID
403:PMID
318:and
310:and
308:CCL2
148:, a
144:, a
494:doi
452:PMC
442:doi
393:doi
357:doi
353:271
316:C5a
296:Use
55:RNA
31:An
530::
508:.
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490:14
488:.
474:^
460:.
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430:99
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389:20
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371:^
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168:,
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359::
324:L
301:L
290:L
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255:-
253:D
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192:L
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174:L
162:L
126:L
122:D
118:L
114:L
91:L
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61:-
59:L
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