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into a highly reactive form, which causes the label to bind more permanently to the large molecule via a covalent bond. The technique was first described in the 1970s. Molecules that have been used as labels in this process are often analogs of complex molecules, in which certain functional groups
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Akiyama, S.; Cornwell, M. M.; Kuwano, M.; Pastan, I.; Gottesman, M. M. (1988). "Most drugs that reverse multidrug resistance also inhibit photoaffinity labeling of P-glycoprotein by a vinblastine analog".
192:"5-Azido-2-aminopyridine, a New Nitrene/Nitrenium Ion Photoaffinity Labeling Agent That Exhibits Reversible Intersystem Crossing between Singlet and Triplet Nitrenes"
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38:. The "label" attaches to the molecule loosely and reversibly, and has an inactive site which can be converted using
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159:"The Ugi four-component reaction enables expedient synthesis and comparison of photoaffinity probes"
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Panov, M. S.; Voskresenska, V. D.; Ryazantsev, M. N.; Tarnovsky, A. N.; Wilson, R. M. (2013).
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technique used to attach "labels" to the active site of a large molecule, especially a
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Bush, J. T.; Walport, L. J.; McGouran, J. F.; Leung, I. K. H.; Berridge, G. (2013).
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Ruoho, A. E.; Kiefer, H.; Roeder, P. E.; Singer, S. J. (1973).
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are replaced with a photoreactive group, such as an
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