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containing clonal nucleic acid populations was first described in patents by Brown, et al.. (assigned to
Genomic Nanosystems), however these are in liquid phase. Clusters are distinct in that they are based on solid-phase amplification of single DNA molecules where the DNA has been covalently attached to a surface. This technology, initially coined "DNA colony generation", had been invented and developed in late 1996 at Glaxo-Welcome's Geneva Biomedical Research Institute (GBRI), by Dr
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The terminology and distinction between 'polony' and 'cluster' have become confused recently. Growth of clonal copies of DNA on bead surfaces remains to be generically named although some also seek to name this technique as a "polony" method. The concept of localizing and analyzing regions
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Polonies are discrete clonal amplifications of a single DNA molecule, grown in a gel matrix. This approach greatly improves the signal-to-noise ratio. Polonies can be generated using several techniques that include solid-phase
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38:(acquired by Solexa), which generate DNA on a solid-phase surface by bridge amplification, are generally referred to as "clusters".
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and Dr
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DNA colony massively parallel sequencing ams98 presentation
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is a contraction of "polymerase colony," a small colony of
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Adessi C, Matton G, Ayala G, et al. (October 2000).
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