62:
1519:
71:
Structurally, RHAU is a 1008 amino acid-long modular protein. It consists of a ~440-amino acid helicase core comprising all signature motifs of the DEAH-box family of helicases with N- and C-terminal flanking regions of ~180 and ~380 amino acids, respectively. Like all the DEAH-box proteins, the
88:. RHAU binds G4-nucleic acid with sub-nanomolar affinity and unwinds G4 structures much more efficiently than double-stranded nucleic acid. Consistent with these biochemical observations, RHAU was also identified as the major source of tetramolecular RNA-resolving activity in HeLa cell lysates.
190:"G4 resolvase 1 binds both DNA and RNA tetramolecular quadruplex with high affinity and is the major source of tetramolecular quadruplex G4-DNA and G4-RNA resolving activity in HeLa cell lysates"
95:(SGs) upon translational arrest induced by various environmental stresses. A region of the first 105 amino acid was shown to be critical for RNA binding and re-localisation to SGs.
346:
1039:
339:
569:
538:
149:"The DEXH protein product of the DHX36 gene is the major source of tetramolecular quadruplex G4-DNA resolving activity in HeLa cell lysates"
332:
1238:
1129:
314:
1124:
1394:
1146:
1509:
1194:
511:
1116:
72:
helicase associated domain is located adjacent to the helicase core region and occupies 75% of the C-terminal region.
295:"Characterizing functional domains of the RNA helicase RHAU involved in subcellular localization and RNA interaction"
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1343:
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730:
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363:
112:
Abdelhaleem M, Maltais L, Wain H (June 2003). "The human DDX and DHX gene families of putative RNA helicases".
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244:"Recruitment of the RNA helicase RHAU to stress granules via a unique RNA-binding domain"
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1353:
480:
270:
243:
216:
189:
92:
242:
ChalupnĂkovĂĄ K, Lattmann S, Selak N, Iwamoto F, Fujiki Y, Nagamine Y (December 2008).
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634:
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1009:
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911:
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81:
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188:
Creacy SD, Routh ED, Iwamoto F, Nagamine Y, Akman SA, Vaughn JP (December 2008).
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20:
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133:
91:
Previous work showed that RHAU associates with mRNAs and re-localises to
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34:
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30:
147:
Vaughn JP, Creacy SD, Routh ED, et al. (November 2005).
1216:
84:(G4) resolvase activity and specificity for its substrate
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164:
49:family of helicases encoded by the DHX36
66:Schematic representation of RHAU protein
60:
1514:
104:
80:RHAU exhibits a unique ATP-dependent
7:
248:The Journal of Biological Chemistry
194:The Journal of Biological Chemistry
153:The Journal of Biological Chemistry
14:
315:Group Yoshikuni Nagamine homepage
1517:
41:or G4R1) is a 114-kDa human RNA
293:ChalupnĂkovĂĄ, KateĆina (2008).
1:
126:10.1016/S0888-7543(03)00049-1
37:-rich element, also known as
1117:Protein-synthesizing GTPase
320:Group Steven Akman homepage
1571:
18:
1395:MichaelisâMenten kinetics
1176:Guanylate-binding protein
360:acid anhydride hydrolases
33:Helicase associated with
1287:Diffusion-limited enzyme
731:Heterotrimeric G protein
426:Phosphoadenylylsulfatase
403:Thiamine-triphosphatase
16:Enzyme, duplicate en-WP
261:10.1074/jbc.M804857200
207:10.1074/jbc.M806277200
166:10.1074/jbc.C500348200
68:
1380:EadieâHofstee diagram
1313:Allosteric regulation
1158:Polymerization motors
869:Rho family of GTPases
64:
1390:LineweaverâBurk plot
19:For other uses, see
1166:dynamin superfamily
1349:Enzyme superfamily
1282:Enzyme promiscuity
82:guanine-quadruplex
69:
1505:
1504:
1214:
1213:
1210:
1209:
711:
710:
676:
675:
421:Adenylylsulfatase
1562:
1522:
1521:
1513:
1385:HanesâWoolf plot
1328:Enzyme activator
1323:Enzyme inhibitor
1297:Enzyme catalysis
1241:
1234:
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1218:
726:
456:
449:
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254:(50): 35186â98.
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200:(50): 34626â34.
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159:(46): 38117â20.
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1508:
1506:
1501:
1413:Oxidoreductases
1399:
1375:Enzyme kinetics
1363:
1359:List of enzymes
1332:
1301:
1272:Catalytic triad
1250:
1245:
1215:
1206:
1151:
1110:
861:Ras superfamily
850:
834:
814:
760:
750:
742:
707:
672:
628:
609:
555:
512:Plasma membrane
475:
430:
407:
381:Pyrophosphatase
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311:
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93:stress granules
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24:
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5:
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1337:Classification
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459:Cu++ (3.6.3.4)
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309:External links
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1354:Enzyme family
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1326:
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1321:
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1318:Cooperativity
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1277:Oxyanion hole
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763:
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736:
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724:
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669:
666:
664:
661:
659:
656:
654:
651:
649:
646:
644:
641:
640:
638:
636:
635:P-type ATPase
631:
625:
622:
621:
619:
616:
612:
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603:
601:
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593:
591:
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427:
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181:
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131:
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123:
120:(6): 618â22.
119:
115:
108:
105:
98:
96:
94:
89:
87:
83:
75:
73:
67:
63:
56:
54:
52:
48:
44:
40:
36:
32:
28:
22:
1555:G-quadruplex
1491:Translocases
1488:
1475:
1462:
1449:
1436:
1426:Transferases
1423:
1410:
1267:Binding site
1004:
938:
894:
872:
857:Small GTPase
471:Wilson/ATP7B
466:Menkes/ATP7A
288:
251:
247:
197:
193:
183:
156:
152:
142:
117:
113:
107:
90:
85:
79:
70:
65:
26:
25:
1262:Active site
1181:Mitofusin-1
1156:3.6.5.5-6:
1125:Prokaryotic
1534:Categories
1465:Isomerases
1439:Hydrolases
1306:Regulation
1147:Eukaryotic
780:Transducin
617:(3.6.3.10)
356:Hydrolases
99:References
21:Georg Rhau
1344:EC number
1115:3.6.5.3:
855:3.6.5.2:
797:Gustducin
729:3.6.5.1:
563:(3.6.3.9)
483:(3.6.3.8)
386:Inorganic
57:Structure
1540:EC 3.6.4
1368:Kinetics
1292:Cofactor
1255:Activity
391:Thiamine
280:18854321
226:18842585
175:16150737
134:12782131
114:Genomics
86:in vitro
76:Function
47:DEAH-box
43:helicase
1550:Enzymes
1524:Biology
1478:Ligases
1248:Enzymes
1202:Tubulin
1171:Dynamin
1023:other:
703:Katanin
693:Kinesin
668:ATP13A3
663:ATP13A2
398:Apyrase
271:3259895
217:2596407
45:of the
1510:Portal
1452:Lyases
1079:ARL13B
939:RhoBTB
833:α12/13
721:GTPase
698:Myosin
688:Dynein
658:ATP12A
653:ATP11B
648:ATP10A
643:ATP8B1
633:Other
605:ATP1B4
600:ATP1B3
595:ATP1B2
590:ATP1B1
585:ATP1A4
580:ATP1A3
575:ATP1A2
570:ATP1A1
561:Na+/K+
549:ATP2C2
544:ATP2C1
532:ATP2B4
527:ATP2B3
522:ATP2B2
517:ATP2B1
505:ATP2A3
500:ATP2A2
495:ATP2A1
444:ATPase
278:
268:
224:
214:
173:
132:
1404:Types
1135:EF-Tu
1074:SAR1B
1057:RAB27
1052:RAB23
1005:RhoDF
895:RhoUV
878:CDC42
873:Cdc42
859:>
844:GNA13
839:GNA12
824:GNA11
813:αq/11
802:GNAT3
790:GNAT2
785:GNAT1
775:GNAI3
770:GNAI2
765:GNAI1
717:3.6.5
681:3.6.4
624:ATP4A
615:H+/K+
490:SERCA
452:3.6.3
436:3.6.3
413:3.6.2
373:3.6.1
298:(PDF)
39:DHX36
1496:list
1489:EC7
1483:list
1476:EC6
1470:list
1463:EC5
1457:list
1450:EC4
1444:list
1437:EC3
1431:list
1424:EC2
1418:list
1411:EC1
1195:OPA1
1188:and
1140:EF-G
1130:IF-2
1096:Rheb
1084:ARL6
1069:ARF6
1040:NRAS
1035:KRAS
1030:HRAS
1015:RhoD
1010:RhoF
956:RhoH
932:RhoG
917:Rac1
905:RhoV
900:RhoU
883:TC10
819:GNAQ
539:SPCA
366:3.6)
276:PMID
222:PMID
171:PMID
130:PMID
51:gene
27:RHAU
1545:RNA
1190:MX2
1186:MX1
1106:RGK
1101:Rap
1091:Ran
1064:Arf
1047:Rab
1025:Ras
983:Rnd
961:Rho
912:Rac
888:TCL
749:olf
481:Ca+
266:PMC
256:doi
252:283
212:PMC
202:doi
198:283
161:doi
157:280
122:doi
31:RNA
1536::
871::
759:αi
741:αs
719::
442::
364:EC
358::
274:.
264:.
250:.
246:.
234:^
220:.
210:.
196:.
192:.
169:.
155:.
151:.
128:.
118:81
116:.
53:.
35:AU
1512::
1498:)
1494:(
1485:)
1481:(
1472:)
1468:(
1459:)
1455:(
1446:)
1442:(
1433:)
1429:(
1420:)
1416:(
1240:e
1233:t
1226:v
998:3
993:2
988:1
976:C
971:B
966:A
949:2
944:1
927:3
922:2
831:G
811:G
757:G
747:G
739:G
440:4
438:-
362:(
348:e
341:t
334:v
300:.
282:.
258::
228:.
204::
177:.
163::
136:.
124::
29:(
23:.
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