575:
The valley definition defines ΔM as the closest spacing of two peaks of equal intensity with the valley (lowest value of signal) between them less than a specified fraction of the peak height. Typical values are 10% or 50%. The value obtained from a 5% peak width is roughly equivalent to a 10%
554:
in mass spectrometry, therefore it is important to report the method used to determine mass resolution when reporting its value. The two most widely used are the peak width definition and the valley definition.
465:
160:
340:
Where a larger resolution indicates a better separation of peaks. This definition is used in a number of mass spectrometry texts. This use is also implied by the term "high-resolution mass spectrometry."
335:
234:
529:
563:
In the peak width definition, the value of ΔM is the width of the peak measured at a specified fraction of the peak height, for example 0.5%, 5%, 10% or 50%. The latter is called the
360:
Some mass spectrometrists use the definition that is similar to definitions used in some other fields of physics and chemistry. In this case, resolving power is defined as:
1097:
1068:
1039:
946:
643:
614:
348:
separations, although it is important to note that the definitions are not the same. High resolution indicating better peak separation is also used in
1186:
1161:
893:
864:
366:
1136:
1024:
839:
811:
786:
761:
732:
707:
682:
76:
240:
585:
166:
542:. and ion microscopy (SIMS) but not gas chromatography. This definition also appears in some mass spectrometry texts.
21:
476:
1208:
753:
564:
349:
344:
A high value for resolution corresponding to good separation of peaks is similar to the convention used with
534:
Resolution and resolving power, when defined in this way, are consistent with IUPAC recommendations for
1154:
Introduction to mass spectrometry: instrumentation, applications and strategies for data interpretation
885:
539:
40:
36:
1182:
1157:
1132:
1020:
993:
926:
909:
VanLear GE, McLafferty FW (1969). "Biochemical aspects of high-resolution mass spectrometry".
889:
860:
835:
807:
782:
757:
728:
703:
678:
28:
17:
1111:
1082:
1053:
985:
960:
918:
657:
628:
590:
58:
There are two different definitions of resolution and resolving power in mass spectrometry.
470:
The minimum peak separation ΔM which allows to distinguish two ion species is then called:
922:
345:
989:
1202:
47:
1107:
1078:
1049:
956:
653:
624:
827:
1102:
1073:
1044:
976:
Blumberg LM, Kle MS (November 2001). "Metrics of separation in chromatography".
951:
648:
619:
535:
1106:, 2nd ed. (the "Gold Book") (1997). Online corrected version: (2006–) "
1077:, 2nd ed. (the "Gold Book") (1997). Online corrected version: (2006–) "
1048:, 2nd ed. (the "Gold Book") (1997). Online corrected version: (2006–) "
955:, 2nd ed. (the "Gold Book") (1997). Online corrected version: (2006–) "
652:, 2nd ed. (the "Gold Book") (1997). Online corrected version: (2006–) "
623:, 2nd ed. (the "Gold Book") (1997). Online corrected version: (2006–) "
1115:
1086:
1057:
1016:
964:
661:
632:
997:
930:
832:
The
Expanding Role of Mass Spectrometry in Biotechnology, Second Edition
460:{\displaystyle R={\cfrac {M}{\Delta M}}=\mathrm {resolving\ power} }
67:
155:{\displaystyle R={\cfrac {M}{\Delta M}}=\mathrm {resolution} }
550:
There are several ways to define the minimum peak separation
330:{\displaystyle M=\mathrm {mass\ of\ the\ (second)\ peak} }
391:
379:
101:
89:
882:
Computational methods for mass spectrometry proteomics
394:
382:
104:
92:
479:
369:
243:
169:
79:
229:{\displaystyle \Delta M=\mathrm {resolving\ power} }
523:
459:
329:
228:
154:
70:definition for resolution in mass spectrometry is
942:
940:
855:Stroobant, Vincent; Hoffmann, Edmond de (2007).
698:Tureček, František; McLafferty, Fred W. (1993).
675:Mass Spectrometry: Organic Chemical Applications
750:Ionization methods in organic mass spectrometry
35:is a measure of the ability to distinguish two
1179:Fundamentals of contemporary mass spectrometry
857:Mass spectrometry: principles and applications
702:. Sausalito, Calif: University Science Books.
524:{\displaystyle \Delta M=\mathrm {resolution} }
8:
802:Todd, John F. J.; March, Raymond E. (2005).
16:This article is about the quantity used in
1011:Karpas, Zeev; Eiceman, Gary Alan (2005).
777:JURGEN H. GROSS; Jnrgen H. Gross (2004).
489:
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250:
242:
179:
168:
120:
105:
93:
86:
78:
610:
608:
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1079:resolving power in optical spectroscopy
602:
1181:. Chichester: John Wiley & Sons.
1156:. Chichester: John Wiley & Sons.
804:Quadrupole ion trap mass spectrometry
677:. New York: McGraw-Hill. p. 13.
7:
654:resolving power in mass spectrometry
923:10.1146/annurev.bi.38.070169.001445
1103:Compendium of Chemical Terminology
1074:Compendium of Chemical Terminology
1050:resolution in optical spectroscopy
1045:Compendium of Chemical Terminology
952:Compendium of Chemical Terminology
727:. Philadelphia: Lippincott-Raven.
649:Compendium of Chemical Terminology
620:Compendium of Chemical Terminology
517:
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14:
1108:resolving power in ion microscopy
725:Introduction to mass spectrometry
1129:Mass Spectrometry Desk Reference
957:resolution in gas chromatography
806:. New York: Wiley-Interscience.
1131:. Pittsburgh: Global View Pub.
625:resolution in mass spectroscopy
700:Interpretation of mass spectra
308:
287:
54:Resolution and resolving power
1:
990:10.1016/S0021-9673(01)01256-0
779:Mass Spectrometry: A Textbook
586:Resolution (electron density)
748:Ashcroft, Alison E. (1997).
1152:Sparkman, O. David (2007).
781:. Berlin: Springer-Verlag.
22:Resolution (disambiguation)
1225:
1127:David O. Sparkman (2006).
754:Royal Society of Chemistry
565:full width at half maximum
356:Resolving power definition
15:
1013:Ion mobility spectrometry
880:Ingvar Eidhammer (2007).
546:Measuring peak separation
350:ion mobility spectrometry
1116:10.1351/goldbook.I03221
1087:10.1351/goldbook.R05322
1058:10.1351/goldbook.R05319
965:10.1351/goldbook.R05317
673:Biemann, Klaus (1962).
662:10.1351/goldbook.R05321
633:10.1351/goldbook.R05318
1177:Dass, Chhabil (2007).
723:Watson, J. S. (1997).
525:
461:
331:
230:
156:
39:of slightly different
20:. For other uses, see
886:John Wiley & Sons
559:Peak width definition
526:
462:
332:
231:
157:
41:mass-to-charge ratios
859:. London: J. Wiley.
540:optical spectroscopy
477:
367:
241:
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77:
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103:
91:
911:Annu. Rev. Biochem
752:. Cambridge, Eng:
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226:
152:
113:
98:
1209:Mass spectrometry
1188:978-0-471-68229-5
1163:978-0-470-51634-8
895:978-0-470-51297-5
866:978-0-470-03310-4
571:Valley definition
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29:mass spectrometry
18:mass spectrometry
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591:Image resolution
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62:IUPAC definition
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239:
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165:
164:
100:
88:
75:
74:
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56:
25:
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11:
5:
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1201:
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1187:
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1144:
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1119:
1090:
1061:
1032:
1025:
1015:. Boca Raton:
1003:
978:J Chromatogr A
968:
936:
901:
894:
884:. Chichester:
872:
865:
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819:
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787:
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715:
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346:chromatography
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1190:
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1140:
1138:0-9660813-9-0
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1123:
1120:
1117:
1113:
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1105:
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1099:
1094:
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1088:
1084:
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1065:
1062:
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1028:
1026:0-8493-2247-2
1022:
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984:(1–2): 1–11.
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841:0-9742451-2-7
837:
834:. MCC Press.
833:
829:
828:Siuzdak, Gary
823:
820:
815:
813:0-471-48888-7
809:
805:
798:
795:
790:
788:3-540-40739-1
784:
780:
773:
770:
765:
763:0-85404-570-8
759:
755:
751:
744:
741:
736:
734:0-397-51688-6
730:
726:
719:
716:
711:
709:0-935702-25-3
705:
701:
694:
691:
686:
684:0-07-005235-2
680:
676:
669:
666:
663:
659:
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650:
645:
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48:mass spectrum
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917:: 289–322.
597:References
536:microscopy
33:resolution
1017:CRC Press
481:Δ
397:Δ
171:Δ
107:Δ
1203:Category
998:11758739
830:(2006).
580:See also
576:valley.
567:(FWHM).
931:4896241
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1098:IUPAC
1069:IUPAC
1040:IUPAC
947:IUPAC
644:IUPAC
615:IUPAC
68:IUPAC
37:peaks
1183:ISBN
1158:ISBN
1133:ISBN
1021:ISBN
994:PMID
927:PMID
890:ISBN
861:ISBN
836:ISBN
808:ISBN
783:ISBN
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729:ISBN
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518:n
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