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number of times there are no mismatches, single mismatches, 2 mismatches, etc. Variance in allele sizes are used to make inferences about the genetic distance between individuals or populations. By comparing summary statistics at different levels of organization it is possible to make inferences about population histories. For example, we can examine the variance of allele size within a subpopulation as well as within the total population to infer something about population history.
155:). Therefore, the SMM cannot be used to determine the exact number of mutational events between two individuals. For example, individual A might have gained a single additional repeat (from an ancestor who had 9) whereas individual B might have lost a single repeat (from an ancestor who had 11), resulting in both individuals with identical number of microsatellite repeats (that is, 10 repeats for a particular locus).
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135:(i.e. determine relatedness of individuals) or determine genetic distance between groups of individuals. For example, more genetically distant individuals would show larger differences in the size of SSRs than more closely related individuals. Given the underlying assumptions of the SMM, it has been widely adopted for use with
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A number of summary statistics can be used to estimate genetic differentiation using the SMM model. These include number of alleles, observed and expected heterozygosity, and allele frequencies. The SMM model takes into account the frequency of mismatches between microsatellite loci, meaning the
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Molecular markers provide only a βsampleβ of the genetic information in which to compare individuals of populations, and can differ from actual genetic differentiation. For example, it is possible that two individual are identical at a given locus, having the same mutation even from its common
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and including variations that suggest that mutations are split between point mutations that disrupt stretches of repeats and the additions or removal of repeat units. This last assumption provides an explanation for why microsatellites do not evolve into enormous arrays of infinite
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that causes it to change in state, mutations that occur in repetitive regions of the genome will increase or decrease by a single repeat unit at a fixed rate (i.e. by the addition or subtraction of one repeat unit per generation) and these changes in
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states are expressed by an integer (. . . A-1, A, A1, .. .). The model also assumes random mating and that all alleles are selectively equivalent for each locus. The SMM is distinguished from the Kimura-Crow model, also known as the
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under the SMM is, however, complicated by the fact that it is possible to either gain or lose a repeat unit, thus alleles that are identical in size are not necessarily identical by descent (i.e. they show marker-size
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There are limitations associated with various marker types and the number of markers used can heavily influence analytical results (with a higher number of markers generally showing greater ability to resolve genetic
124:) and the mutation rate is fixed, the mean number of different alleles in the population rapidly reaches a peak and plateaus, at which time that value is almost the same as the effective number of alleles.
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Some important caveats and limitations to consider when choosing molecular markers for estimating the relatedness of individuals or distinguishing between populations include the following:
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Estoup, Arnaud; Jarne, Philippe; Cornuet, Jean-Marie (2002). "Homoplasy and mutation model at microsatellite loci and their consequences for population genetics analysis".
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Selkoe, Kimberly A.; Toonen, Robert J. (2006). "Microsatellites for ecologists: a practical guide to using and evaluating microsatellite markers".
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513:"Measuring genetic distances between breeds: use of some distances in various short term evolution models"
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The original SMM has been modified in multiple ways to deal with these short comings, including:
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factoring in the likelihood of large alleles to show higher rates of mutation than small alleles
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247:"Stepwise mutation model and distribution of allelic frequencies in a finite population"
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116:(IAM), in that as the population size increases to infinity, while the product of the N
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Ellegren, Hans (2004). "Microsatellites: simple sequences with complex evolution".
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in a finite population where neutral alleles are produced in step-wise fashion.
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ancestor, but could differ at other loci that were not observed (or sequenced).
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that contain repeat regions, are co-dominate, and have high rates of mutation.
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which has rapidly become widely used for correcting some common SMM errors:
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are not detectable by plain SMM and will produce very incorrect results.
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Laval, Guillaume; SanCristobal, Magali; Chevalet, Claude (2002-07-15).
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90:, that allows for investigation of the equilibrium distribution of
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taking into account the upper size limit to most microsatellites
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131:" (SSRs) between individuals can thus be used to construct
391:(2002). "Sequence divergence of rice microsatellites in
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330:Valdes, A. M.; Slatkin, M.; Freimer, N. B. (1993).
102:The original model assumes that if an allele has a
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may be too technical for most readers to understand
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252:Proceedings of the National Academy of Sciences
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62:Learn how and when to remove this message
46:, without removing the technical details.
216:of large alleles, incorrect guessing of
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204:Van Oosterhout et al. 2004 introduces
201:Piry et al. 1999 introduces Bottleneck
44:make it understandable to non-experts
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646:10.1111/j.1461-0248.2006.00889.x
588:10.1046/j.1365-294x.2002.01576.x
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398:Molecular Genetics and Genomics
127:Differences in the length of "
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518:Genetics Selection Evolution
220:, and typographical errors.
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532:10.1186/1297-9686-34-4-481
395:and other plant species".
348:10.1093/genetics/133.3.737
411:10.1007/s00438-002-0739-5
122:effective population size
458:Nature Reviews Genetics
129:simple sequence repeats
76:stepwise mutation model
274:10.1073/pnas.75.6.2868
137:microsatellite markers
114:infinite alleles model
682:Population genetics
387:Chen, X.; Cho, Y.;
265:1978PNAS...75.2868K
92:allelic frequencies
80:mathematical theory
16:Mathematical theory
575:Molecular Ecology
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582:(9): 1591β1604.
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146:Construction of
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389:McCouch, Susan
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245:(1978-06-01).
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210:null alleles
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176:Null alleles
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84:Motoo Kimura
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159:Limitations
148:phylogenies
133:phylogenies
98:Description
88:Tomoko Ohta
78:(SMM) is a
225:References
183:Extensions
52:April 2018
654:1461-023X
596:0962-1083
541:1297-9686
479:1471-0056
419:1617-4615
356:0016-6731
283:0027-8424
153:homoplasy
676:Category
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604:12207711
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495:11975343
487:15153996
427:12436255
336:Genetics
104:mutation
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261:Bibcode
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109:allele
608:S2CID
491:S2CID
431:S2CID
393:Oryza
313:S2CID
291:68345
287:JSTOR
198:size.
658:PMID
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555:PMID
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