Knowledge (XXG)

Autoclave

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572: 585: 556: 346:, can survive and even reproduce at temperatures found in autoclaves, their growth rate is so slow at the lower temperatures in the less extreme environments occupied by humans that it is unlikely they could compete with other organisms. None of them are known to be infectious or otherwise pose a health risk to humans; in fact, their biochemistry is so different from that of humans, and their multiplication rate is so slow, that microbiologists need not worry about them. 293: 457: 423:. Many medical-grade autoclaves are therefore limited to running regulator-approved cycles. Because they are optimized for continuous hospital use, they favor rectangular designs, require demanding maintenance regimens, and are costly to operate. (A properly calibrated medical-grade autoclave uses thousands of gallons of water each day, independent of task, with correspondingly high electric power consumption.) 448:
medical-grade autoclaves used in their research labs were each consuming 700 gallons of water and 90 kWh of electricity per day (1,134MWh of electricity and 8.8 million gallons of water total), because they consumed energy and water continuously, even when not in use. UCR's research-grade autoclaves performed the same tasks with equal effectiveness, but used 83% less energy and 97% less water.
216:(but not eliminate) potentially infectious agents by using pressurized steam and superheated water. A new generation of waste converters is capable of achieving the same effect without a pressure vessel to sterilize culture media, rubber material, gowns, dressings, gloves, etc. It is particularly useful for materials that cannot withstand the higher temperature of a hot air oven. 597: 499:
difficult challenge device, named the Bowie-Dick device after its inventors, is also used to verify a full cycle. This contains a full sheet of chemical indicator placed in the center of a stack of paper. It is designed specifically to prove that the process achieved full temperature and time required for a normal minimum cycle of 134 Â°C for 3.5–4 minutes.
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where efficiency, ease-of-use, and flexibility are at a premium. Research-grade autoclaves may be configured for "pass-through" operation. This makes it possible to maintain absolute isolation between "clean" and potentially contaminated work areas. Pass-through research autoclaves are especially important in
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A notable recent and increasingly popular application of autoclaves is the pre-disposal treatment and sterilization of waste material, such as pathogenic hospital waste. Machines in this category largely operate under the same principles as conventional autoclaves in that they are able to neutralize
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In 2016, the Office of Sustainability at the University of California, Riverside (UCR) conducted a study of autoclave efficiency in their genomics and entomology research labs, tracking several units' power and water consumption. They found that, even when functioning within intended parameters, the
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Research-grade autoclaves—which are not approved for use in sterilizing instruments that will be directly used on humans—are primarily designed for efficiency, flexibility, and ease-of-use. They display a wide range of designs and sizes, and are frequently tailored to their use and load type. Common
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Autoclaves used in education, research, biomedical research, pharmaceutical research and industrial settings (often called "research-grade" autoclaves) are used to sterilize lab instruments, glassware, culture media, and liquid media. Research-grade autoclaves are increasingly used in these settings
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change color once the correct conditions have been met, indicating that the object inside the package, or under the tape, has been appropriately processed. Autoclave tape is only a marker that steam and heat have activated the dye. The marker on the tape does not indicate complete sterility. A more
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There are physical, chemical, and biological indicators that can be used to ensure that an autoclave reaches the correct temperature for the correct amount of time. If a non-treated or improperly treated item can be confused for a treated item, then there is the risk that they will become mixed up,
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As steam enters the chamber, it fills the upper areas first as it is less dense than air. This process compresses the air to the bottom, forcing it out through a drain which often contains a temperature sensor. Only when air evacuation is complete does the discharge stop. Flow is usually controlled
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values are set for the number of minutes of sterilization equivalent to 121 Â°C (250 Â°F) at 103 kPa (14.9 psi) above atmospheric pressure for 15 minutes. Since exact temperature control is difficult, the temperature is monitored, and the sterilization time adjusted accordingly.
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due to environmental and health concerns about the combustion by-products emitted by incinerators, especially from the small units which were commonly operated at individual hospitals. Incineration or a similar thermal oxidation process is still generally mandated for pathological waste and other
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It is very important to ensure that all of the trapped air is removed from the autoclave before activation, as trapped air is a very poor medium for achieving sterility. Steam at 134 Â°C (273 Â°F) can achieve a desired level of sterility in three minutes, while achieving the same level of
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Autoclaves are also widely used to cure composites, especially for melding multiple layers without any voids that would decrease material strength, and in the vulcanization of rubber. The high heat and pressure that autoclaves generate help to ensure that the best possible physical properties are
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when run on the factory default setting. The study found that 18 of 18 (100%) mock patient loads (6 PPE, 6 linen, and 6 liquid loads) passed sterilization tests with the optimized parameters compared to only 3 of 19 (16%) mock loads that passed with use of the factory default settings.
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bags often have a "sterilization indicator mark" that typically darkens when the bag and its contents have been adequately processed. Comparing the marks on an unprocessed bag (L) and on a bag that has been properly cycled (R) will reveal an obvious visual
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repeatable. Manufacturers of spars for sailboats have autoclaves well over 50 feet (15 m) long and 10 feet (3 m) wide, and some autoclaves in the aerospace industry are large enough to hold whole airplane fuselages made of layered composites.
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Garibaldi, Brian T.; Reimers, Mallory; Ernst, Neysa; Bova, Gregory; Nowakowski, Elaine; Bukowski, James; Ellis, Brandon C.; Smith, Chris; Sauer, Lauren; Dionne, Kim; Carroll, Karen C.; Maragakis, Lisa L.; Parrish, Nicole M. (February 2017).
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Autoclaves are found in many medical settings, laboratories, and other places that need to ensure the sterility of an object. Many procedures today employ single-use items rather than sterilizable, reusable items. This first happened with
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The machine on the right is an autoclave used for processing substantial quantities of laboratory equipment prior to reuse, and infectious material prior to disposal. (The machines on the left and in the middle are washing
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Stovetop autoclaves used in poorer or non-medical settings do not always have automatic air removal programs. The operator is required to manually perform steam pulsing at certain pressures as indicated by the gauge.
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Australian/New Zealand Standard: Office-based health care facilities— Reprocessing of reusable medical and surgical instruments and equipment, and maintenance of the associated environment (AS/NZS 4815:2006)
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variations include either a cylindrical or square pressure chamber, air- or water-cooling systems, and vertically or horizontally opening chamber doors (which may be electrically or manually powered).
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Achieved with a vacuum pump. It starts with a vacuum followed by a steam pulse followed by a vacuum followed by a steam pulse. The number of pulses depends on the particular autoclave and cycle chosen.
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and other products that may be damaged by steam must also be sterilized another way. In all autoclaves, items should always be separated to allow the steam to penetrate the load evenly.
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Immerse in sodium hydroxide (NaOH)20 and heat in a gravity displacement autoclave at 121°C for 30 min; clean; rinse in water and subject to routine sterilization.
1178:"Validation of Autoclave Protocols for Successful Decontamination of Category A Medical Waste Generated from Care of Patients with Serious Communicable Diseases" 252:, but bleed holes are sometimes used. As the steam and air mix, it is also possible to force out the mixture from locations in the chamber other than the bottom. 584: 45: 2567: 2546: 1239: 787: 279:
Similar to the superatmospheric cycles, but chamber pressure never exceeds atmospheric pressure until they pressurize up to the sterilizing temperature.
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Air dilution by using a series of steam pulses, in which the chamber is alternately pressurized and then depressurized to near atmospheric pressure.
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designed to melt only after being subjected to a given temperature for the relevant holding time. If the alloy melts, the change will be visible.
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In order to sterilize items effectively, it is important to use optimal parameters when running an autoclave cycle. A 2017 study performed by the
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is a machine used to carry out industrial and scientific processes requiring elevated temperature and pressure in relation to
508: 1142: 374:). Autoclaves are of particular importance in poorer countries due to the much greater amount of equipment that is re-used. 329: 2675: 2511: 2400: 1741: 1324: 680: 2215: 1233: 536: 460: 305: 107: 58: 1292: 129:(about double atmospheric pressure) depending on the size of the load and the contents. The autoclave was invented by 2680: 2291: 2260: 2172: 86: 31: 2415: 1746: 992: 1817: 1420: 901: 2250: 2167: 1631: 118:. Industrial autoclaves are used in industrial applications, especially in the manufacturing of composites. 44: 1853: 1601: 1570: 996: 621:
Menge, John A. (1984). "Chapter 9: Inoculom Production". In Powel, Conway L.; Bagyaraj, D. Joseph (eds.).
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sterility in hot air requires two hours at 160 Â°C (320 Â°F). Methods of air removal include:
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functions as a single-purpose autoclave designed for the sterilization of liquid waste and effluent.
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Joint Technical Committee HE-023, Processing of Medical and Surgical Instruments (6 April 2006),
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Typical loads include laboratory glassware, other equipment and waste, surgical instruments, and
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Many autoclaves are used to sterilize equipment and supplies by subjecting them to pressurized
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Hugo WB (July 1991). "A brief history of heat and chemical preservation and disinfection".
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Dental equipment in an autoclave to be sterilized for 2 hours at 150 to 180 degrees Celsius
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Autoclaving is often used to sterilize medical waste prior to disposal in the standard
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To prove sterility, biological indicators are used. Biological indicators contain
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at 121 Â°C (250 Â°F) for 30–60 minutes at a pressure of 205 kPa or 2.02
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In dentistry, autoclaves provide sterilization of dental instruments.
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stream. This application has become more common as an alternative to
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handles) are commonly single-use rather than reusable items (see
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Cutaway illustration of a jacketed rectangular-chamber autoclave
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A vacuum pump sucks air or air/steam mixtures from the chamber.
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very toxic or infectious medical waste. For liquid waste, an
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Illustration of a cylindrical-chamber pass-through autoclave
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Horizontal high-capacity autoclave with cylindrical chamber
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Cutaway illustration of a cylindrical-chamber autoclave
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unit tested the ability of pass-through autoclaves to
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United States Department of Health and Human Services
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World Health Organization. pp. 29–32 926:: CS1 maint: numeric names: authors list ( 909:, Standards Australia/Standards New Zealand 848:. iSmithers Rapra Publishing. p. 161. 133:in 1879, although a precursor known as the 2519: 2506: 2495: 2327: 2314: 2147: 2134: 1807: 1794: 1366: 1355: 1339: 1325: 1317: 1240:Centers for Disease Control and Prevention 1090:Microbiology and Molecular Biology Reviews 300:A medical autoclave is a device that uses 43: 1268:"The Autoclave, effectivity and low cost" 1209: 1117: 1052: 149:meaning key, thus a self-locking device. 2657:Instruments used in medical laboratories 240:Downward displacement (or gravity-type): 2173:Inductively coupled plasma (ICP) device 613: 551: 164:Video demonstrating how autoclaves work 2221:Transmission electron microscope (TEM) 919: 683:. Pasteur Institute. 9 November 2018. 36: 1143:"Laboratory autoclaves: a case study" 814:. Lippincott Williams & Wilkins. 205:in the chemical and food industries. 145:, ultimately meaning self, and Latin 7: 1141:Faugeroux, Delphine; Wells, Barbra. 942:"All American Autoclave Sterilizers" 747:. Etymonline.com. September 16, 2018 415:In most of the industrialized world 681:"Charles Chamberland, the inventor" 561:Stovetop autoclaves, also known as 141:in 1679. The name comes from Greek 27:Temperature and pressure instrument 2216:Scanning electron microscope (SEM) 769:. Consolidated Machine Corporation 719:10.1111/j.1365-2672.1991.tb04657.x 25: 2261:Thermogravimetric analyzer (TGA) 2071:Nuclear magnetic resonance (NMR) 1182:Journal of Clinical Microbiology 595: 583: 570: 554: 535:(F-nought) value to control the 328:, such as those associated with 707:Journal of Applied Bacteriology 687:from the original on 2021-11-22 506:of a heat-resistant bacterium, 407:effluent decontamination system 225:effluent decontamination system 808:Seymour Stanton Block (2001). 659:. Prentice Hall. p. 334. 509:Geobacillus stearothermophilus 1: 967:"How Does an Autoclave Work?" 409:is the equivalent hardware. 1027:Cowan, D. A. (2004-02-01). 1004:Communicable Diseases (CDS) 745:Online Etymology Dictionary 565:—the simplest of autoclaves 520:will change the color of a 2697: 324:are inactivated. However, 32:Autoclave (disambiguation) 29: 2654: 2522: 2505: 2494: 2416:Time-domain reflectometer 2326: 2313: 2183:Liquid chromatograph (LC) 2146: 2133: 1806: 1793: 1365: 1354: 1045:10.1016/j.tim.2003.12.002 655:Black, Jacquelyn (1993). 516:when incubated and their 494:on medical packaging and 419:autoclaves are regulated 330:Creutzfeldt–Jakob disease 42: 948:. Red Hill General Store 270:Superatmospheric cycles: 2251:Melting-point apparatus 1632:Cryogenic storage dewar 1274:. Kalstein France. 2022 385:products (such as some 2188:Mass spectrometer (MS) 2178:Gas chromatograph (GC) 1033:Trends in Microbiology 842:R. B. Simpson (2002). 767:"Sterilization Cycles" 470:Johns Hopkins Hospital 465: 297: 276:Subatmospheric cycles: 165: 116:hydrothermal synthesis 91: 2563:Acid-resistant gloves 2244:differential scanning 1102:10.1128/MMBR.00016-10 946:AllAmericanCanner.com 459: 398:municipal solid waste 295: 163: 89: 2676:Laboratory equipment 2140:Analytical chemistry 1642:Laminar flow cabinet 1348:Laboratory equipment 1194:10.1128/JCM.02161-16 356:surgical instruments 30:For other uses, see 2512:Personal protective 1421:Meker–Fisher burner 1248:. 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Elsevier B.V 1284: 1259: 1225: 1188:(2): 545–551. 1167: 1133: 1096:(3): 417–433. 1076: 1019: 995:(1999-03-26). 984: 958: 933: 892: 868: 854: 834: 820: 800: 779: 758: 732: 697: 672: 665: 647: 633: 612: 611: 609: 606: 605: 604: 601: 594: 592: 589: 582: 580: 576: 569: 567: 560: 553: 549: 546: 540: 532: 496:autoclave tape 473:biocontainment 453: 450: 428: 425: 381:heat is used, 289: 286: 281: 280: 277: 274: 271: 268: 265: 259: 256: 255:Steam pulsing: 253: 250:solenoid valve 241: 232: 229: 170: 167: 135:steam digester 82: 81: 76: 72: 71: 66: 62: 61: 56: 52: 51: 48: 26: 24: 14: 13: 10: 9: 6: 4: 3: 2: 2693: 2682: 2679: 2677: 2674: 2673: 2671: 2658: 2653: 2639: 2636: 2634: 2631: 2629: 2626: 2624: 2621: 2619: 2616: 2614: 2611: 2610: 2608: 2604: 2594: 2591: 2589: 2586: 2584: 2581: 2579: 2576: 2574: 2571: 2569: 2566: 2564: 2561: 2560: 2558: 2554: 2548: 2547:Safety shower 2545: 2543: 2540: 2538: 2535: 2533: 2530: 2528: 2525: 2524: 2521: 2518: 2515: 2508: 2504: 2497: 2493: 2479: 2476: 2474: 2471: 2470: 2468: 2464: 2458: 2457:Wire stripper 2455: 2453: 2450: 2448: 2445: 2443: 2440: 2439: 2437: 2433: 2427: 2424: 2422: 2419: 2417: 2414: 2412: 2409: 2407: 2404: 2402: 2399: 2397: 2394: 2392: 2389: 2387: 2384: 2383: 2381: 2377: 2371: 2368: 2366: 2363: 2361: 2358: 2356: 2353: 2349: 2346: 2344: 2341: 2340: 2339: 2336: 2335: 2333: 2329: 2325: 2321: 2316: 2312: 2298: 2295: 2293: 2290: 2288: 2287:Spiral plater 2285: 2283: 2280: 2278: 2275: 2274: 2272: 2268: 2262: 2259: 2257: 2254: 2252: 2249: 2245: 2242: 2241: 2240: 2237: 2236: 2234: 2232: 2228: 2222: 2219: 2217: 2214: 2213: 2211: 2209: 2205: 2199: 2196: 2194: 2191: 2189: 2186: 2184: 2181: 2179: 2176: 2174: 2171: 2169: 2166: 2164: 2161: 2159: 2156: 2155: 2153: 2151:Compositional 2149: 2145: 2141: 2136: 2132: 2118: 2115: 2113: 2110: 2108: 2105: 2103: 2100: 2099: 2097: 2093: 2087: 2084: 2082: 2079: 2077: 2074: 2072: 2069: 2067: 2064: 2062: 2059: 2058: 2056: 2054: 2050: 2044: 2041: 2039: 2036: 2034: 2031: 2029: 2026: 2024: 2021: 2019: 2016: 2014: 2011: 2009: 2006: 2005: 2003: 1999: 1993: 1990: 1988: 1985: 1983: 1980: 1978: 1975: 1974: 1972: 1968: 1962: 1959: 1957: 1954: 1952: 1949: 1947: 1944: 1942: 1939: 1937: 1934: 1932: 1929: 1927: 1924: 1922: 1919: 1917: 1914: 1913: 1911: 1909: 1905: 1899: 1896: 1894: 1891: 1889: 1886: 1884: 1881: 1880: 1878: 1874: 1868: 1865: 1863: 1860: 1859: 1857: 1855: 1851: 1845: 1842: 1841: 1839: 1835: 1829: 1826: 1824: 1821: 1819: 1816: 1815: 1813: 1809: 1805: 1801: 1796: 1792: 1778: 1775: 1773: 1770: 1768: 1765: 1763: 1760: 1758: 1755: 1753: 1750: 1748: 1745: 1743: 1740: 1738: 1735: 1733: 1730: 1728: 1725: 1723: 1720: 1718: 1715: 1713: 1710: 1708: 1705: 1703: 1700: 1698: 1695: 1693:Balance brush 1692: 1690: 1687: 1685: 1682: 1681: 1679: 1675: 1669:Weighing dish 1668: 1666:Weighing boat 1665: 1663: 1660: 1658: 1655: 1653: 1650: 1648: 1645: 1643: 1640: 1638: 1635: 1633: 1630: 1628: 1625: 1624: 1622: 1616: 1613: 1612: 1609: 1603: 1600: 1598: 1595: 1593: 1590: 1588: 1585: 1582: 1580: 1577: 1574: 1572: 1569: 1566: 1563: 1560: 1558: 1557:Burette clamp 1555: 1553: 1550: 1548: 1545: 1542: 1541: 1539: 1533: 1531: 1528: 1525: 1524: 1521: 1515: 1512: 1510: 1507: 1505: 1502: 1500: 1497: 1495: 1492: 1490: 1487: 1485: 1482: 1480: 1477: 1475: 1472: 1470: 1467: 1465: 1462: 1461: 1459: 1453: 1450: 1449: 1446: 1440: 1437: 1435: 1432: 1430: 1427: 1424: 1422: 1419: 1417: 1414: 1412: 1409: 1407: 1404: 1402: 1399: 1397: 1394: 1392: 1391:Bunsen burner 1389: 1387: 1384: 1383: 1381: 1375: 1372: 1371: 1368: 1364: 1357: 1353: 1349: 1342: 1337: 1335: 1330: 1328: 1323: 1322: 1319: 1300: 1299: 1298:ScienceDirect 1294: 1288: 1285: 1273: 1269: 1263: 1260: 1247: 1243: 1241: 1235: 1229: 1226: 1221: 1217: 1212: 1207: 1203: 1199: 1195: 1191: 1187: 1183: 1179: 1171: 1168: 1155: 1151: 1144: 1137: 1134: 1129: 1125: 1120: 1115: 1111: 1107: 1103: 1099: 1095: 1091: 1087: 1080: 1077: 1072: 1068: 1064: 1060: 1055: 1050: 1046: 1042: 1038: 1034: 1030: 1023: 1020: 1016: 1005: 998: 994: 988: 985: 972: 968: 962: 959: 947: 943: 937: 934: 929: 923: 905: 904: 896: 893: 882: 878: 872: 869: 857: 851: 847: 846: 845:Rubber Basics 838: 835: 823: 817: 813: 812: 804: 801: 789: 783: 780: 768: 762: 759: 746: 742: 736: 733: 728: 724: 720: 716: 712: 708: 701: 698: 686: 682: 676: 673: 668: 666:9780135829172 662: 658: 651: 648: 636: 630: 627:. CRC Press. 626: 625: 624:VA Mycorrhiza 617: 614: 607: 598: 593: 586: 581: 573: 568: 564: 557: 552: 547: 545: 538: 537:sterilization 529: 527: 523: 519: 515: 511: 510: 505: 500: 497: 493: 489: 485: 482: 478: 477:decontaminate 474: 471: 462: 461:Sterilization 458: 451: 449: 445: 441: 439: 435: 426: 424: 422: 418: 417:medical-grade 413: 410: 408: 403: 399: 394: 392: 388: 384: 380: 375: 373: 369: 366:holders, and 365: 361: 357: 353: 347: 345: 344: 339: 335: 331: 327: 323: 319: 315: 311: 307: 303: 294: 287: 285: 278: 275: 272: 269: 266: 263: 260: 257: 254: 251: 247: 242: 239: 238: 237: 230: 228: 226: 221: 217: 213: 211: 210:medical waste 206: 204: 200: 196: 195:body piercing 192: 189:fabrication, 188: 184: 180: 176: 168: 150: 148: 144: 140: 136: 132: 128: 124: 119: 117: 113: 109: 108:sterilization 105: 101: 97: 88: 80: 77: 75:Related items 73: 70: 67: 63: 60: 59:Sterilization 57: 53: 46: 41: 33: 19: 2618:Fire blanket 2556:Eye and hand 2542:Rubber apron 2406:Oscilloscope 2370:Potentiostat 2297:Plate reader 2193:pH indicator 2163:CHN analyzer 2158:AutoAnalyzer 1951:Round-bottom 1844:Boston round 1707:Filter paper 1688: 1662:Refrigerator 1579:Retort stand 1547:Clamp holder 1543:Beaker clamp 1509:Vortex mixer 1504:Stirring rod 1499:Static mixer 1429:Teclu burner 1303:. Retrieved 1296: 1293:"Sterilizer" 1287: 1276:. Retrieved 1271: 1262: 1250:. Retrieved 1237: 1228: 1185: 1181: 1170: 1158:. Retrieved 1153: 1149: 1136: 1093: 1089: 1079: 1039:(2): 58–60. 1036: 1032: 1022: 1014: 1008:. Retrieved 1003: 987: 975:. Retrieved 971:ScienceEquip 970: 961: 950:. Retrieved 945: 936: 911:, retrieved 902: 895: 884:. Retrieved 881:ScienceDaily 880: 871: 859:. Retrieved 844: 837: 825:. Retrieved 810: 803: 792:. Retrieved 782: 771:. Retrieved 761: 749:. Retrieved 744: 735: 710: 706: 700: 689:. Retrieved 675: 657:Microbiology 656: 650: 638:. Retrieved 623: 616: 530: 507: 501: 490: 486: 467: 446: 442: 440:facilities. 430: 414: 411: 402:incineration 395: 376: 348: 341: 299: 282: 262:Vacuum pumps 234: 222: 218: 214: 207: 175:microbiology 172: 146: 142: 120: 95: 93: 2606:Other items 2532:Face shield 2379:Measurement 2360:Galvanostat 2320:Electronics 2270:Other items 2256:Thermometer 2239:Calorimeter 2168:Colorimeter 2112:Gas syringe 2095:Other items 2023:Eye dropper 1898:Watch glass 1883:Evaporating 1862:Cold finger 1677:Other items 1583:Screw clamp 1575:Pinch clamp 1564:Flask clamp 1514:Wash bottle 1469:Homogenizer 713:(1): 9–18. 464:difference. 427:In research 383:heat-labile 288:In medicine 231:Air removal 187:prosthetics 139:Denis Papin 104:temperature 2670:Categories 2537:Respirator 2478:Test probe 2396:Multimeter 2208:Microscopy 2028:Eudiometer 1992:Separatory 1961:Volumetric 1926:Erlenmeyer 1854:Condensers 1818:Dean–Stark 1767:Wire brush 1727:Microscope 1722:Centrifuge 1697:Cork borer 1652:Petri dish 1627:Agar plate 1614:Containers 1597:Wire gauze 1434:Water bath 1396:Desiccator 1278:2023-10-21 1156:(6): 10–12 1010:2002-02-05 952:2022-04-30 886:2020-01-13 861:19 January 827:19 January 794:2017-04-20 773:2009-06-30 691:2021-12-15 608:References 578:machines.) 518:metabolism 246:steam trap 223:A thermal 18:Sterilizer 2628:Fume hood 2573:Glove box 2426:Voltmeter 1811:Apparatus 1800:Glassware 1689:Autoclave 1684:Aspirator 1637:Incubator 1571:Iron ring 1494:Sonicator 1464:Chemostat 1406:Hot plate 1202:0095-1137 1110:1092-2172 1063:0966-842X 1054:10566/147 514:germinate 358:(such as 334:Cereulide 306:sterilize 191:tattooing 112:vulcanize 96:autoclave 38:Autoclave 2527:Lab coat 2452:Tweezers 2442:Heat gun 2198:pH meter 2107:Bell jar 1987:Dropping 1941:Florence 1931:Fernbach 1893:Syracuse 1752:Scoopula 1702:Crucible 1411:Lab oven 1272:Kalstein 1252:June 11, 1220:27927920 1128:20805405 1071:15040324 922:citation 751:June 11, 685:Archived 640:June 11, 539:cycle. F 387:plastics 377:Because 310:bacteria 183:medicine 179:mycology 65:Inventor 2466:General 2386:Ammeter 2086:Thistle 2043:Pipette 2018:Cuvette 2008:Burette 1977:BĂĽchner 1970:Funnels 1956:Schlenk 1936:Fleaker 1916:BĂĽchner 1837:Bottles 1757:Spatula 1747:Stopper 1717:Forceps 1617:Storage 1534:Holders 1454:Shakers 1425:Striker 1373:Heaters 1360:General 1305:11 June 1211:5277525 1160:May 24, 1119:2937522 977:11 June 913:11 June 727:1894581 368:scalpel 360:forceps 338:archaea 314:viruses 102:and/or 2500:Safety 2102:Beaker 2081:Thiele 2066:Cragie 2061:Drying 1982:Hirsch 1946:Retort 1908:Flasks 1876:Dishes 1867:Liebig 1828:Kipp's 1742:Splint 1552:Tripod 1530:Clamps 1526:Stands 1489:Shaker 1451:Mixers 1376:Dryers 1218:  1208:  1200:  1126:  1116:  1108:  1069:  1061:  852:  818:  725:  663:  631:  504:spores 364:needle 326:prions 322:spores 320:, and 203:retort 197:, and 147:clavis 2435:Tools 2053:Tubes 1888:Petri 1242:(CDC) 1238:U.S. 1146:(PDF) 1000:(PDF) 907:(pdf) 526:alloy 438:BSL-4 434:BSL-3 391:Paper 318:fungi 302:steam 248:or a 244:by a 143:auto- 2117:Vial 2076:Test 1712:File 1416:Kiln 1307:2024 1254:2024 1216:PMID 1198:ISSN 1162:2017 1124:PMID 1106:ISSN 1067:PMID 1059:ISSN 979:2024 928:link 915:2024 863:2013 850:ISBN 829:2013 816:ISBN 753:2024 723:PMID 661:ISBN 642:2024 629:ISBN 379:damp 193:and 185:and 177:and 169:Uses 55:Uses 1206:PMC 1190:doi 1114:PMC 1098:doi 1049:hdl 1041:doi 715:doi 436:or 304:to 127:atm 94:An 2672:: 1295:. 1270:. 1244:. 1236:. 1214:. 1204:. 1196:. 1186:55 1184:. 1180:. 1154:20 1152:. 1148:. 1122:. 1112:. 1104:. 1094:74 1092:. 1088:. 1065:. 1057:. 1047:. 1037:12 1035:. 1031:. 1013:. 1002:. 969:. 944:. 924:}} 920:{{ 879:. 743:. 721:. 711:71 709:. 522:pH 362:, 316:, 312:, 212:. 181:, 1340:e 1333:t 1326:v 1309:. 1281:. 1256:. 1222:. 1192:: 1164:. 1130:. 1100:: 1073:. 1051:: 1043:: 981:. 955:. 930:) 889:. 865:. 831:. 797:. 776:. 755:. 729:. 717:: 694:. 669:. 644:. 541:0 533:0 264:: 34:. 20:)

Index

Sterilizer
Autoclave (disambiguation)

Sterilization
Charles Chamberland
Waste autoclave

ambient pressure
temperature
sterilization
vulcanize
hydrothermal synthesis
saturated steam
atm
Charles Chamberland
steam digester
Denis Papin
microbiology
mycology
medicine
prosthetics
tattooing
body piercing
funerary practice
retort
medical waste
effluent decontamination system
steam trap
solenoid valve
Vacuum pumps

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