127:
TBE can be diluted to 1X prior to use in electrophoresis, 0.5x is acceptable as well. Higher concentrations will result in poor results due to excessive heat generation.
74:, the role of the EDTA is to protect the nucleic acids against enzymatic degradation. But since Mg is also a co-factor for many useful DNA-modifying enzymes such as
203:
58:-acid solutions are effective buffers for slightly basic conditions, which keep DNA deprotonated and soluble in water. EDTA is a
163:
220:
204:
https://web.archive.org/web/20171107223153/https://www.eeb.ucla.edu/Faculty/Barber/Web%20Protocols/Protocol7.pdf
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89:
More recently discovered substitutes for TBE and TAE buffers for electrophoresis are available.
186:
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51:
24:
82:, its concentration in TBE or TAE buffers is generally kept low (typically at around 1
79:
214:
83:
70:(Mg). As these ions are necessary co-factors for many enzymes, including contaminant
47:
139:, lithium borate buffer, a similar buffer containing lithium ions in place of Tris
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106:
43:
32:
182:
164:"History and principles of conductive media for standard DNA electrophoresis"
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67:
59:
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63:
145:, a similar buffer containing acetic acid in place of boric acid
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99:
55:
36:
28:
117:
46:buffers are often used in procedures involving
8:
154:
93:Recipe (1 liter of 5X stock solution)
7:
14:
162:Brody, J.R.; Kern, S.E. (2004).
42:In molecular biology, TBE and
1:
237:
124:Adjust pH to 8.3 by HCl.
102:(CAS# 77-86-1, free base)
183:10.1016/j.ab.2004.05.054
50:, the most common being
27:containing a mixture of
76:restriction enzymes
66:, particularly of
109:(CAS# 10043-35-3)
228:
221:Buffer solutions
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201:
195:
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168:
159:
116:(CAS# 60-00-4) (
21:Tris/Borate/EDTA
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112:20 ml of 0.5 M
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80:DNA polymerases
52:electrophoresis
25:buffer solution
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11:
5:
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48:nucleic acids
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171:Anal Biochem
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62:of divalent
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20:
16:
15:
177:(1): 1–13.
150:References
143:TAE buffer
107:boric acid
105:27.5 g of
33:boric acid
137:LB buffer
100:Tris base
72:nucleases
68:magnesium
29:Tris base
215:Category
191:15351274
131:See also
98:54 g of
60:chelator
64:cations
23:, is a
189:
167:(PDF)
187:PMID
120:8.0)
114:EDTA
78:and
56:Tris
37:EDTA
35:and
179:doi
175:333
86:).
44:TAE
19:or
17:TBE
217::
185:.
173:.
169:.
118:pH
84:mM
54:.
39:.
31:,
193:.
181::
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