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Although THP-1 cells are of the same lineage, mutations can cause differences as the progeny proliferates. In general, THP-1 cells exhibit a large, round, single-cell morphology. The cells were derived from the peripheral blood of a 1-year-old human male with acute monocytic leukemia. Some of their
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1640 + 10% FBS + 2mM L-Glutamine. The average doubling time is 19 to 50 hours. 1 mM sodium pyruvate, penicillin (100 units/ml) and streptomycin (100 μg/ml) are also commonly added to inhibit bacterial contamination. Cultures should be maintained at cell densities in the range 2-9x10 cells/ml at
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for THP-1 is HLA-A*02:01; A*24:02; B*15:11; B*35:01; C*03:03; DRB1*01:01; DRB1*15:01; DQB1*05:01; DQB1*06:02; DPB1*02:01; DPB1*04:02 (in the German
Collection of Microorganisms and Cell Cultures (DSMZ) cell bank). This HLA type can change depending on the reference biorepository, due to loss of
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Differentiation into immature dendritic cells, using recombinant human interleukin 4 (rhIL-4) and recombinant human granulocyte macrophage colony-stimulating factor (rhGM-CSF), and mature dendritic cells using rhIL-4, rhGM-CSF, recombinant human tumour necrotic factor α (rhTNF-α) and
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THP-1 cells are used as a models to study the monocyte-macrophage differentiation process, and as a model to examine some macrophage-related physiological processes, for example the macrophage cholesterol efflux.
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Tsuchiya S, Yamabe M, Yamaguchi Y, Kobayashi Y, Konno T, Tada K (August 1980). "Establishment and characterization of a human acute monocytic leukemia cell line (THP-1)".
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THP-1 cells are of human origin, and no evidence has been found for the presence of infectious viruses or toxic products. The ATCC Biosafety recommendation is level 1.
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Berges C, Naujokat C, Tinapp S, Wieczorek H, Höh A, Sadeghi M, Opelz G, Daniel V (August 2005). "A cell line model for the differentiation of human dendritic cells".
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heterozygosity in multiple chromosomal regions, as THP-1 from the
American Type Culture Collection (ATCC) do not express the HLA-A*24:02 and B*35:01 alleles.
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Polarization into the M1 phenotype by incubation with IFN-γ and LPS, or to the M2 phenotype by incubation with interleukin 4 and interleukin 13
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Auwerx J (January 1991). "The human leukemia cell line, THP-1: a multifacetted model for the study of monocyte-macrophage differentiation".
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284:"M1 and M2 macrophages derived from THP-1 cells differentially modulate the response of cancer cells to etoposide"
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487:"6-Dihydroparadol, a Ginger Constituent, Enhances Cholesterol Efflux from THP-1-derived Macrophages"
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Wang D, Hiebl V, Ladurner A, Latkolik SL, Bucar F, Heiß EH, Dirsch VM, Atanasov AG (May 2018).
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Noronha N, Ehx G, Meunier MC, Laverdure JP, Thériault C, Perreault C (March 2020).
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production on phagocytosis and differentiation into macrophage-like cells
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Genin M, Clement F, Fattaccioli A, Raes M, Michiels C (August 2015).
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THP-1 can provide continuous culture when grown in suspension;
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physiology (both for latex beads and sensitized erythrocytes).
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Positive detection of alpha-naphthyl butyrate esterase and
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100:analysis of protein-protein interactions, and
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66:Learn how and when to remove this message
151:Restoration of the response of purified
29:This article includes a list of general
491:Molecular Nutrition & Food Research
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125:while lacking surface and cytoplasmic
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35:it lacks sufficient corresponding
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374:International Journal of Cancer
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92:patient. It is used to test
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347:10.1016/j.bbrc.2005.05.171
202:. Cells are non-adherent.
301:10.1186/s12885-015-1546-9
90:acute monocytic leukemia
50:more precise citations.
503:10.1002/mnfr.201800011
253:10.1002/ijc.2910260208
214:Research applications
113:characteristics are:
102:immunohistochemistry
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189:Growth Information
98:immunocytochemical
387:10.1002/ijc.32967
198:37 °C, 5% CO
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288:BMC Cancer
223:References
176:Ionomycin.
162:Increased
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429:2018
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