132:(SIM) chromatogram is similar to an EIC/XIC, with the exception that the mass spectrometer is operated in SIM mode, such that only preselected m/z values are detected in the analysis. SIM experiments can be performed using mass spectrometry (MS) or tandem mass spectrometry (MS/MS) instruments. They are more common on MS instruments. This differs significantly from the extracted-ion chromatogram mentioned above in that only data for the ion(s) of interest are collected in a SIM experiment; for extracted-ion chromatograms (EIC or XIC), data for an entire mass range are collected during the run and then examined for analytes of interest after the completion of the run.
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60:. In this case, the x-axis represents retention time, analogous to any other chromatogram. The y-axis represents signal intensity or relative signal intensity. There are many different types of metrics that this intensity may represent, depending on what information is extracted from each mass spectrum.
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The total ion current (TIC) chromatogram represents the summed intensity across the entire range of masses being detected at every point in the analysis. The range is typically several hundred mass-to-charge units or more. In complex samples, the TIC chromatogram often provides limited information as
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in the gas phase and a specific fragment ion is monitored. This experiment has very high specificity because the SRM chromatogram represents only ions of a particular mass that fragment in a manner that produce a very specific product mass. This type of experiment can only be performed using tandem
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of the instrument collecting the data. This is useful for re-examining data to detect previously-unsuspected analytes, to highlight potential isomers, resolve suspected co-eluting substances, or to provide clean chromatograms of compounds of interest. An extracted-ion chromatogram is generated by
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In an extracted-ion chromatogram (EIC or XIC), also called a reconstructed-ion chromatogram (RIC), one or more m/z values representing one or more analytes of interest are recovered ('extracted') from the entire data set for a chromatographic run. The total intensity or base peak intensity within a
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separating the ions of interest from a data file containing the full mass spectrum over time after the fact; this is different from selected-ion chromatograms, discussed below, in which data is collected only for specific m/z values. A closely related term is extracted-compound chromatogram (ECC).
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The base peak chromatogram is similar to the TIC chromatogram, however it monitors only the most intense peak in each spectrum. This means that the base peak chromatogram represents the intensity of the most intense peak at every point in the analysis. Base peak chromatograms often have a cleaner
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and the y-axis represents signal intensity. The source data contains mass information; however, it is not graphically represented in a mass chromatogram in favor of visualizing signal intensity versus time. The most common use of this data representation is when mass spectrometry is used in
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mass spectrometry. The technology progress in the MS/MS area lead to the development of MRM, Multiple
Reaction Monitoring, which allows simultaneous detection of several coeluting analytes with different parent and/or product ions.
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is used and a specific product ion of a specific parent ion is detected. The mass of the parent analyte is first selected while other ions are filtered away. The parent analyte ion is then
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mass tolerance window around a particular analyte's mass-to-charge ratio is plotted at every point in the analysis. The size of the mass tolerance window typically depends on the
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look and thus are more informative than TIC chromatograms because the background is reduced by focusing on a single analyte at every point.
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Murray, Kermit K.; Boyd, Robert K.; Eberlin, Marcos N.; Langley, G. John; Li, Liang; Naito, Yasuhide (2013).
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186:(July 1970). "Computer evaluation of continuously scanned mass spectra of gas chromatographic effluents".
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multiple analytes elute simultaneously, obscuring individual species.
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144:(SRM) experiment is very similar to the SIM experiment except that
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An example total ion current chromatogram from an LC-MS analysis.
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An example extracted-ion chromatogram from an LC-MS analysis.
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An example base peak chromatogram from an LC-MS analysis.
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Selected-reaction monitoring chromatogram (SRM, MRM)
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124:Selected-ion monitoring chromatogram (SIM)
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98:Extracted-ion chromatogram (EIC or XIC)
54:liquid chromatography–mass spectrometry
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64:Total ion current (TIC) chromatogram
58:gas chromatography–mass spectrometry
326:Compendium of Chemical Terminology
297:Compendium of Chemical Terminology
220:Compendium of Chemical Terminology
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48:conjunction with some form of
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142:selected-reaction monitoring
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257:Pure and Applied Chemistry
270:10.1351/PAC-REC-06-04-06
146:tandem mass spectrometry
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331:selected ion monitoring
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130:selected-ion monitoring
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81:Base peak chromatogram
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189:Analytical Chemistry
202:10.1021/ac60290a009
182:Hites, Ronald A.;
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357:Mass spectrometry
225:total ion current
33:mass spectrometry
29:mass chromatogram
18:Total ion current
16:(Redirected from
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351:Category
157:See also
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292:IUPAC
215:IUPAC
275:ISSN
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