226:, and then centrifuged them to remove the solid material. Next, the remaining liquid mixture was injected into chicks. The chicks developed sarcomas. The causative agent in the liquid was a virus, this is now called the Rous sarcoma virus (RSV).
245:
A function for Src tyrosine kinases in normal cell growth was first demonstrated with the binding of family member p56lck to the cytoplasmic tail of the CD4 and CD8 co-receptors on T-cells. Src tyrosine kinases also transmit
284:
The first sequence of v-Src was published in 1980 and the characterization of sites for tyrosine phosphorylation in the transforming protein of Rous sarcoma virus and its normal cellular homologue was published in 1981.
507:
Czernilofsky AP, Levinson AD, Varmus HE, Bishop JM, Tischer E, Goodman HM (September 1980). "Nucleotide sequence of an avian sarcoma virus oncogene (src) and proposed amino acid sequence for gene product".
413:
Stehelin D, Fujita DJ, Padgett T, Varmus HE, Bishop JM (1977). "Detection and enumeration of transformation-defective strains of avian sarcoma virus with molecular hybridization".
184:
as it triggers uncontrolled growth in abnormal host cells. It was the first retroviral oncogene to be discovered. The src gene was taken up by RSV and incorporated into its
561:"Characterization of sites for tyrosine phosphorylation in the transforming protein of Rous sarcoma virus (pp60v-src) and its normal cellular homologue (pp60c-src)"
69:
273:) which is only activated under certain circumstances where it is required (e.g. growth factor signaling). v-Src is therefore an instructive example of an
294:
270:
116:
262:
on the basal surface of the cell. These structures are correlated with increased invasiveness, a process thought to be essential for metastasis.
218:
first proposed that viruses can cause cancer. He proved it in 1911 and was later awarded the Nobel prize in 1966. Chickens grow a tumor called a
62:
450:"The CD4 receptor is complexed in detergent lysates to a protein-tyrosine kinase (pp58) from human T lymphocytes"
269:
inhibitory phosphorylation site (tyrosine-527), and is therefore constitutively active as opposed to normal Src (
98:
33:
572:
517:
461:
258:. Hallmarks of v-Src induced transformation are rounding of the cell and the formation of actin rich
215:
623:
541:
166:
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Smart JE, Oppermann H, Czernilofsky AP, Purchio AF, Erikson RL, Bishop JM (October 1981).
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Rudd CE, Trevillyan JM, Dasgupta JD, Wong LL, Schlossman SF (July 1988).
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274:
259:
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conferring it with the advantage of being able to stimulate uncontrolled
181:
86:
364:"Transduction of a cellular oncogene: the genesis of Rous sarcoma virus"
234:
Further research done later on by others showed that RSV was a type of
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189:
529:
185:
57:
330:
362:
Swanstrom, R; Parker, R C; Varmus, H E; Bishop, J M (May 1983).
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gene in RSV is required for the formation of cancer.
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23:
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192:of host cells, providing abundant cells for fresh
19:Tyrosine-protein kinase transforming protein Src
368:Proceedings of the National Academy of Sciences
8:
315:"Retroviral oncogenes: a historical primer"
173:that causes a type of cancer in chickens.
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584:
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389:
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199:The src gene is not essential for RSV
15:
222:. Rous collected and ground up these
7:
14:
250:-dependent signals central to
1:
565:Proc. Natl. Acad. Sci. U.S.A
454:Proc. Natl. Acad. Sci. U.S.A
427:10.1016/0042-6822(77)90250-1
640:
313:Vogt PK (September 2012).
203:but it greatly increases
123:
238:. It was found that the
586:10.1073/pnas.78.10.6013
475:10.1073/pnas.85.14.5190
381:10.1073/pnas.80.9.2519
169:(RSV) that encodes a
117:viral: 0.01 - 0.01 Mb
577:1981PNAS...78.6013S
522:1980Natur.287..198C
466:1988PNAS...85.5190R
277:whereas c-Src is a
216:Francis Peyton Rous
167:Rous sarcoma virus
34:Rous sarcoma virus
516:(5779): 198–203.
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374:(9): 2519–2523.
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319:Nat. Rev. Cancer
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265:v-Src lacks the
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171:tyrosine kinase
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5:
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571:(10): 6013–7.
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460:(14): 5190–4.
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279:proto-oncogene
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207:when present.
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421:(2): 675–84.
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325:(9): 639–48.
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256:proliferation
254:movement and
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201:proliferation
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70:RefSeq (Prot)
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27:
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568:
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220:fibrosarcoma
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115:
49:Alt. symbols
137:Swiss-model
75:NP_056888.1
24:Identifiers
301:References
267:C-terminal
236:retrovirus
133:Structures
128:Search for
111:Chromosome
93:Other data
624:Oncogenes
260:podosomes
211:Discovery
205:virulence
194:infection
182:oncogenic
165:found in
99:EC number
52:pp60v-src
618:Category
415:Virology
349:22898541
289:See also
275:oncogene
248:integrin
230:Function
224:sarcomas
178:src gene
147:InterPro
104:2.7.10.2
29:Organism
605:6273838
573:Bibcode
546:4231060
538:6253794
518:Bibcode
494:2455897
462:Bibcode
400:6302692
340:3428493
190:mitosis
143:Domains
82:UniProt
63:1491925
603:
596:348967
593:
544:
536:
510:Nature
492:
485:281714
482:
435:190771
433:
398:
391:393857
388:
347:
337:
186:genome
87:P00526
58:Entrez
41:Symbol
542:S2CID
295:c-Src
271:c-Src
240:v-Src
161:is a
159:v-Src
44:v-src
601:PMID
534:PMID
490:PMID
431:PMID
396:PMID
345:PMID
252:cell
176:The
163:gene
591:PMC
581:doi
526:doi
514:287
480:PMC
470:doi
423:doi
386:PMC
376:doi
335:PMC
327:doi
180:is
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