127:(LA) mainly causes N2a differentiation through phosphorylation of the ERK pathway and the Akt pathway. Inhibition of only the ERK pathway will prevent LA-induced differentiation from occurring, while inhibition of the Akt pathway will not prevent LA-induced differentiation. LA causes differentiation through the production of
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N2a cells are able to rapidly, reliably, and easily differentiate, making them effective for use in research applications relating to neurons and neuronal disorders. Serum withdrawal is a common method of inducing differentiation of N2a cells, involving removal of the serum that the cells are grown
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causes N2a differentiation and growth through activation of the PI3K/Akt pathway. It does so by activating Akt while inactivating glycogen synthase kinase-3β (GSK-3β), an inhibitor of the Akt pathway. Emodin specifically causes phosphorylation of cAMP-responsive element binding protein (CREB), an
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LePage KT, Dickey RW, Gerwick WH, Jester EL, Murray TF (2005). "On the use of neuro-2a neuroblastoma cells versus intact neurons in primary culture for neurotoxicity studies".
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113:, which is regulated by the Atk pathway, and expression of glucose transporters in N2a cells, leading to differentiation. It also increases the activity of
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Salto, Rafael; Vílchez, Jose D.; Girón, María D.; Cabrera, Elena; Campos, Nefertiti; Manzano, Manuel; Rueda, Ricardo; López-Pedrosa, Jose M. (2015-08-12).
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Wang, Xiaohui; Wang, Zhuyao; Yao, Yuzhen; Li, Jingjin; Zhang, Xiaojin; Li, Chuanfu; Cheng, Yunlin; Ding, Guoxian; Liu, Li; Ding, Zhengnian (2011-05-01).
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inhibit differentiation of N2a cells that are differentiated through serum-withdrawal differentiation. HDL does so through inhibiting the function of
415:"Signalling pathways leading to neuroblastoma differentiation after serum withdrawal: HDL blocks neuroblastoma differentiation by inhibition of EGFR"
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since initial collection, can exhibit responses to toxins that differ from those of neuronal cells in a live organism. Synthesizing large amounts of
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Ogrodnik M, Salmonowicz H, Brown R, Turkowska J, Sredniawa W, Pattabiraman S, Amen T, Abraham AC, Eichler N, Lyakhovetsky R, Kaganovich D (2014).
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Tremblay, Roger G.; Sikorska, Marianna; Sandhu, Jagdeep K.; Lanthier, Patricia; Ribecco-Lutkiewicz, Maria; Bani-Yaghoub, Mahmud (2010-01-30).
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Park, Shin-Ji; Jin, Mei Ling; An, Hyun-Kyu; Kim, Kyoung-Sook; Ko, Min Jung; Kim, Cheol Min; Choi, Young Whan; Lee, Young-Choon (2015-02-19).
109:(HMB) causes N2a growth through activation and phosphorylation of the PI3K/Akt and MAPK/ERK signaling pathways. HMB causes activation of
605:"Dynamic JUNQ inclusion bodies are asymmetrically inherited in mammalian cell lines through the asymmetric partitioning of vimentin"
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148:(EGFR) by preventing EGFR phosphorylation. This prevents EGFR from causing activation of the Akt and ERK pathways.
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556:"Interpretation and applicability of microRNA data to the context of Alzheimer's and age-related diseases"
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in response to environmental factors. The differentiated cells have many properties of neurons, including
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334:"Emodin induces neurite outgrowth through PI3K/Akt/GSK-3β-mediated signaling pathways in Neuro2a cells"
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in to activate the signaling pathways governing differentiation. N2a cells have been used to study
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Sun, Ying-Jian; Long, Ding-Xin; Li, Wei; Hou, Wei-Yuan; Wu, Yi-Jun; Shen, Jian-Zhong (2010-02-01).
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462:"Effects of avermectins on neurite outgrowth in differentiating mouse neuroblastoma N2a cells"
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Proceedings of the
National Academy of Sciences of the United States of America
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Evangelopoulos, Maria
Elephteria; Weis, Joachim; Krüttgen, Alex (2005-05-05).
229:"β-Hydroxy-β-Methylbutyrate (HMB) Promotes Neurite Outgrowth in Neuro2a Cells"
195:. One specific research applications is the differentiation of N2a cells into
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phosphoinositide-3-kinase/protein kinase B (PI3K/Akt) signaling pathways.
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Originating from a mouse, the N2a cell line has a neuronal and amoeboid
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379:. Including the Special Section: 11th European Symposium on Calcium.
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166:(P-gp) in N2a cells, a major contributor to N2a cell differentiation.
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61:. The differentiation of N2a cells is caused by activation of the
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509:"Differentiation of mouse Neuro 2A cells into dopamine neurons"
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Biochimica et
Biophysica Acta (BBA) - Molecular Cell Research
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important molecule in the differentiation of neurons.
162:(DOM) inhibit N2a differentiation by suppressing
85:) that can alter cell morphology and physiology.
89:Factors that Affect Differentiation of N2a Cells
77:, N2a cells are susceptible to viruses (such as
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142:High density lipoproteins (HDL)
131:which activate the ERK pathway.
525:10.1016/j.jneumeth.2009.11.004
199:for potential use in treating
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478:10.1016/j.toxlet.2009.10.021
389:10.1016/j.bbamcr.2011.01.027
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254:10.1371/journal.pone.0135614
53:morphology, allowing it to
34:) are a fast-growing mouse
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180:outgrowth, neurotoxicity,
107:β-Hydroxy-β-methylbutyrate
45:Differentiation properties
678:Cellosaurus entry for N2a
191:, and the diagnosing of
673:N2a at ECACC (89121404)
632:10.1073/pnas.1324035111
129:reactive oxygen species
431:10.1038/sj.onc.1208494
668:N2a at ATCC (CCL-131)
572:10.18632/aging.100131
171:Research applications
30:cells (also known as
338:Neuroscience Letters
623:2014PNAS..111.8049O
245:2015PLoSO..1035614S
201:Parkinson's Disease
182:Alzheimer's disease
554:Provost P (2010).
466:Toxicology Letters
154:(AVM) derivatives
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186:adenoviral
160:doramectin
158:(ABM) and
152:Avermectin
83:poliovirus
533:0165-0270
486:0378-4274
439:1476-5594
397:0167-4889
358:0304-3940
263:1932-6203
156:abamectin
117:, mainly
71:passaging
51:stem cell
39:cell line
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590:20375468
541:19903493
494:19879343
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419:Oncogene
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281:26267903
233:PLOS ONE
65:and the
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81:and
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