160:
89:
Concentration of borate and NaCl as well as the pH can vary, and the resulting solution would still be referred to as "borate buffered saline". Borate concentration (giving buffering capacity) can vary from 10 mM to 100 mM. As BBS is used to emulate physiological conditions (as in animal or human
90:
body), the pH value is slightly alkaline, ranging from 8.0 to 9.0. NaCl gives the isotonic (mostly used 150 mM NaCl corresponds to physiological conditions: 0.9% NaCl) salt concentration.
86:
The simplest way to prepare a BBS solution is to use BBS tablets. They are formulated to give a ready to use borate buffered saline solution upon dissolution in 500 ml of deionized water.
54:
BBS has many uses because it is isotonic and has a strong bactericidal effect. It can be used to dilute substances and has applications in coating procedures. Additives such as
201:
225:
194:
40:
220:
35:
within a relatively narrow range. Borate buffers have an alkaline buffering capacity in the 8–10 range.
187:
58:
and milk powder can be used to add to BBS's functionality as a washing buffer or blocking buffer.
137:
171:
159:
127:
119:
24:
55:
132:
107:
214:
167:
28:
36:
141:
123:
71:
77:
32:
175:
108:"Antimicrobial activity of borate-buffered solutions"
106:Houlsby, R. D; Ghajar, M; Chavez, G. O (1986).
195:
8:
202:
188:
66:The following is a sample recipe for BBS:
131:
98:
112:Antimicrobial Agents and Chemotherapy
7:
156:
154:
174:. You can help Knowledge (XXG) by
14:
158:
1:
31:techniques to maintain the
242:
153:
46:of 9.14 at 25 °C.
17:Borate buffered saline
124:10.1128/aac.29.5.803
83:Adjust pH to pH 8.2
226:Biochemistry stubs
183:
182:
233:
221:Buffer solutions
204:
197:
190:
162:
155:
146:
145:
135:
103:
241:
240:
236:
235:
234:
232:
231:
230:
211:
210:
209:
208:
151:
149:
105:
104:
100:
96:
64:
52:
44:
12:
11:
5:
239:
237:
229:
228:
223:
213:
212:
207:
206:
199:
192:
184:
181:
180:
163:
148:
147:
97:
95:
92:
81:
80:
74:
63:
60:
56:Polysorbate 20
51:
48:
42:
13:
10:
9:
6:
4:
3:
2:
238:
227:
224:
222:
219:
218:
216:
205:
200:
198:
193:
191:
186:
185:
179:
177:
173:
170:article is a
169:
164:
161:
157:
152:
143:
139:
134:
129:
125:
121:
117:
113:
109:
102:
99:
93:
91:
87:
84:
79:
75:
73:
70:10 mM Sodium
69:
68:
67:
61:
59:
57:
49:
47:
45:
38:
34:
30:
27:used in some
26:
22:
19:(abbreviated
18:
176:expanding it
168:biochemistry
165:
150:
118:(5): 803–6.
115:
111:
101:
88:
85:
82:
65:
53:
50:Applications
20:
16:
15:
29:biochemical
215:Categories
94:References
37:Boric acid
62:Contents
142:3729341
76:150 mM
23:) is a
140:
133:284157
130:
72:borate
39:has a
25:buffer
166:This
172:stub
138:PMID
78:NaCl
128:PMC
120:doi
21:BBS
217::
136:.
126:.
116:29
114:.
110:.
41:pK
33:pH
203:e
196:t
189:v
178:.
144:.
122::
43:a
Text is available under the Creative Commons Attribution-ShareAlike License. Additional terms may apply.