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Cellulose, dextran, agarose, and other insoluble complexes are unaffected because they compose inert matrices, hence why they are so often derivatized with strong and weak cation and anion exchangers in chromatography. DEAE-C beads have diethylaminoethyl chains covalently bound to oxygen atoms on the
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DEAE-C is a weak anion exchanger. This exchange is utilized to separate proteins that have faintly differing charges. Like all anion exchangers, the resin carries a positive charge that interacts favorably with negative charges. The positive charge of DEAE cellulose is due to a protonated amine
51:(DEAE) and lock negatively charged proteins or nucleic acids into the matrix. The proteins are released from the resin by increasing the salt concentration of the solvent or changing the pH of the solution as to change the charge on the protein.
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animal cells with foreign DNA. It is added to solution containing DNA meant for transfection. It binds and interacts with negatively charged DNA molecules and via an unknown mechanism brings about the uptake of
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DEAE-C is commonly commercially available as DE52 and DE53. These resins are prepared preswollen although cellulose exchangers swell in a strong basic environment to increase access to binding sites.
285:
Reuveny, S.; Silberstein, L.; Shahar, A.; Freeman, E.; Mizrahi, A. (Feb 1982). "DE-52 and DE-53 Cellulose
Microcarriers: I. Growth of Primary and Established Anchorage-Dependent Cells".
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of the protein of interest. The resin is a weak exchanger because it is only partially ionized over most pH values, and an efficient separation with DEAE-C
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group. To ensure that the resin is protonated and positively charged, the chromatography should be performed at least 2 pH units below the
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Rousseau, Ronald W.; Ferrell, James K.; Reardon, Robert F. (1984-06-01). "Synthesis of diethylaminoethyl cellulose on cotton fabric".
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Peterson, Elbert A.; Sober, Herbert A. (1956). "Chromatography of
Proteins. I. Cellulose Ion-exchange Adsorbents".
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of the amine group, 10. The strength of the bond between the resin and protein is highly dependent on the
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Schematic structure of DEAE-C: positively charged diethylaminoethanol groups can bind negative ions
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by the cell. This procedure is highly suited for transient transfection used for various
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110:(DEAE-D) is a positively charged dextran derivative that can be used for
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Industrial & Engineering
Chemistry Product Research and Development
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is 8.4-8.8, though the range for DEAE-C varies between manufacturers.
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Fundamental laboratory approaches for biochemistry and biotechnology
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67:) with 2-chlorotriethylamine, illustrated as following :
348:"DEAE and CM Bio-Gel ® A Ion Exchange Gels Instruction Manual"
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DEAE-C is synthesized by an alkali-catalyzed reaction of
411:
Gulick, Tod (2003). "Transfection Using DEAE-Dextran".
126:, and many other applications. DEAE-D is also used for
8:
314:Journal of the American Chemical Society
261:"Whatman® anion exchange cellulose DE52"
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179:requires a specific, narrow pH range.
385:. Amersham Biosciences. Archived from
154:are commonly used in chromatography.
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14:
413:Current Protocols in Cell Biology
94:of 11.5. The buffering range for
1:
201:Size-exclusion chromatography
425:10.1002/0471143030.cb2004s19
171:range in the column and the
450:. Hoboken, NJ: John Wiley.
158:Ion-exchange chromatography
37:ion-exchange chromatography
25:Diethylaminoethyl cellulose
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31:) is a positively charged
446:Ninfa, Alexander (2010).
118:, protein stabilization,
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41:column chromatography
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150:, DEAE-650 and DEAE-
326:10.1021/ja01585a016
239:10.1021/i300014a015
124:flocculating agents
49:diethylaminoethanol
419:: 20.4.1–20.4.10.
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457:978-0-470-08766-4
143:Other derivatives
137:molecular biology
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483:Cellulose ethers
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376:"DEAE-Dextran"
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320:(4): 751–755.
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233:(2): 250–252.
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177:chromatography
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148:DEAE-Sepharose
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96:diethanolamine
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394:. Retrieved
387:the original
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358:. Retrieved
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293:(2): 92–98.
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268:. Retrieved
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187:subunits of
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128:transfecting
122:prevention,
120:dyslipidemia
116:gene therapy
114:production,
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39:, a type of
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90:DE52 has a
55:Preparation
45:derivatized
472:Categories
212:References
478:Cellulose
334:0002-7863
247:0196-4321
189:cellulose
185:D-glucose
139:studies.
61:cellulose
433:18228428
299:20170418
287:In Vitro
195:See also
152:Sephadex
35:used in
355:Bio-Rad
112:vaccine
108:Dextran
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396:11 May
360:11 May
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270:11 May
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102:DEAE-D
29:DEAE-C
390:(PDF)
379:(PDF)
351:(PDF)
295:JSTOR
106:DEAE-
78:Types
47:with
33:resin
452:ISBN
429:PMID
398:2016
362:2016
330:ISSN
272:2016
243:ISSN
421:doi
322:doi
235:doi
165:pKa
92:pKa
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173:pI
169:pH
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Text is available under the Creative Commons Attribution-ShareAlike License. Additional terms may apply.
