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The HL-60 cultured cell line provides a continuous source of human cells for studying the molecular events of myeloid differentiation and the effects of physiologic, pharmacologic, and virologic elements on this process. HL-60 cell model was used to study the effect of
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receptors, which are expressed on cell surface. The requirement for insulin and transferrin is absolute, as HL-60 proliferation immediately ceases if either of these compounds is removed from the serum-free culture media. With this line, differentiation to mature
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promyelocytic morphology. Subsequent evaluation, including the karyotype that showed absence of the defining t(15;17) translocation, concluded that HL-60 cells are from a case of AML FAB-M2 (now referred to as AML with maturation (WHO)).
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of cells and is especially useful in dielectrophoresis studies, which require an aqueous environment with suspended and round cells. Furthermore, these cells have been used in order to investigate whether intracellular
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Breitman, T, S. Collins, B. Keene (1980). "Replacement of serum by insulin and transferrin supports growth and differentiation of the human promyelocytic leukemia cell line, HL-60".
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282:"Temporal and Spatial Distribution of DNA Topoisomerase II Alters During Proliferation, Differentiation, and Apoptosis in HL-60 Cells"
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that has been used for laboratory research on blood cell formation and physiology. HL-60 proliferates continuously in suspension
149:"Characterization of the continuous, differentiating myeloid cell line (HL-60) from a patient with acute promyelocytic leukemia"
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Chromatin and gene expression profiling in HL-60 cells and differentiated cells derived from these has been performed recently.
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Teif V.B., Mallm J.P., Sharma T., Mark Welch D.B., Rippe K., Eils R., Langowski J., Olins A.L., Olins D.E. (2017).
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is about 36–48 hours. The cell line was derived from a 36-year-old woman who was originally reported to have
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400:"Oxidative Stress-Induced Caspases are Regulated in Human Myeloid HL-60 Cells by Calcium Signal"
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Dalton WT, Jr; Ahearn, MJ; McCredie, KB; Freireich, EJ; Stass, SA; Trujillo, JM (January 1988).
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Sugimoto, K, K. Yamada, M. Egashira, Y. yazaki, H. Hirai, A. Kikuchi and K. Oshimi (1998).
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445:"Nucleosome repositioning during differentiation of a human myeloid leukemia cell line"
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González D., Bejarano I., Barriga C., Rodríguez A.B., Pariente J.A. (2010).
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192:"HL-60 cell line was derived from a patient with FAB-M2 and not FAB-M3"
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337:"Detection of cellular responses to toxicants by dielectrophoresis"
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Ratanachoo, K., Gascoyne, P.R.C. and
Ruchirawat, M. (2002).
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Gallagher R, Collins S, Trujillo J, et al. (1979).
55:Proliferation of HL-60 cells occurs through the
76:. Other compounds like 1,25-dihydroxyvitamin D
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491:: CS1 maint: multiple names: authors list (
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82:12-O-tetradecanoylphorbol-13-acetate
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404:Current Signal Transduction Therapy
47:. HL-60 cells predominantly show
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461:10.1080/19491034.2017.1295201
353:10.1016/S0005-2736(02)00494-7
245:10.1016/0014-4827(80)90296-7
41:acute promyelocytic leukemia
209:10.1182/blood.V71.1.242.242
168:10.1182/blood.V54.3.713.713
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509:Cellosaurus entry for HL60
416:10.2174/157436210791112172
100:phenotypes, respectively.
45:MD Anderson Cancer Center
299:10.1182/blood.V91.4.1407
123:reactive oxygen species
341:Biochim. Biophys. Acta
121:activation induced by
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72:(DMSO), or
57:transferrin
132:References
98:eosinophil
96:-like and
94:macrophage
84:(TPA) and
33:antibiotic
110:apoptosis
90:monocytic
25:cell line
518:Category
479:28406749
371:12175928
22:leukemia
470:5403151
449:Nucleus
362:2726261
308:9454772
253:6988226
218:3422031
119:caspase
115:calcium
61:insulin
43:at the
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86:GM-CSF
286:Blood
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154:Blood
18:HL-60
493:link
475:PMID
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367:PMID
345:1564
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59:and
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