455:
binding to double-stranded DNA. The ability of the dyes to detect DNA at low concentrations was evaluated using two new metrics, absolute fluorescence enhancement (AFE) and relativefluorescence enhancement (RFE). They were tested in qPCR experiments showing some important differences in the sensitivity and qPCR efficiency, which facilitate the DNA marker selection for analytical purposes. This study showed that Sybr Green exhibits the highest fluorescence enhancement (AFE) and provided the best characteristic for PCR methods. The second best was TOPhBu, which is close in values to Sybr Green.
391:
179:
685:
407:
27:
309:
454:
A comprehensive Study of
Thiazole-Orange-Based DNA Dyes were published, involving SYBR Safe, SYBR Green, PicoGreen, SYTO-16, SYTO-9 and a new derivative TOPhBu. Here, the synthesis of these compounds are published and characterised spectro-scopically to quantify their fluorescence enhancement upon
703:
Singer VL; Lawlor TE; Yue S (February 1999). "Comparison of SYBR Green I nucleic acid gel stain mutagenicity and ethidium bromide mutagenicity in the
Salmonella/mammalian microsome reverse mutation assay (Ames test)".
486:, which measures the ability of chemicals to cause mutations, when assayed at the same concentration SYBR Green I was on the order of 30 times less mutagenic than ethidium bromide.
202:
InChI=1S/C32H37N4S/c1-5-20-35(22-13-21-33(2)3)31-23-25(24-32-34(4)29-18-11-12-19-30(29)37-32)27-16-9-10-17-28(27)36(31)26-14-7-6-8-15-26/h6-12,14-19,23-24H,5,13,20-22H2,1-4H3/q+1
322:
100:
383:= 520 nm). The stain preferentially binds to double-stranded DNA, but will stain single-stranded (ss) DNA with lower performance. SYBR Green can also stain
218:
439:
in order to visualise the DNA present. In addition to labelling pure nucleic acids, SYBR Green can also be used for labelling of DNA within cells for
778:
402:. The SYBR green in the sample binds to the sperm DNA and, once bound, fluoresces giving off green light when illuminated by blue light
558:"Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications"
193:
773:
390:
329:
157:
654:
Domahidy, Farkas; Kovács, Beatrix; Cseri, Levente; Katona, Gergely; Rózsa, Balázs; et al. (19 June 2024).
368:
471:, as both safer to work with and free from the complex waste disposal issues of ethidium bromide. However any
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689:
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688: This article incorporates text from this source, which is available under the
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427:. It is used as a dye for the quantification of double stranded DNA in some methods of
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26:
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375:. The resulting DNA-dye-complex best absorbs 497 nanometer blue light (λ
468:
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132:
451:
treatment may be required to reduce background from RNA in the cells.
111:
299:
Except where otherwise noted, data are given for materials in their
448:
389:
99:
89:
273:
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372:
162:
435:. Higher concentrations of SYBR Green can be used to stain
475:
capable of binding DNA with high affinity is a possible
656:"Comprehensive Study of Thiazole-Orange-Based DNA Dyes"
394:
A sample of herring sperm stained with SYBR Green in a
317:
226:
CCCN(CCCN(C)C)C1=CC(=Cc2sc3ccccc32C)c2ccccc2N1c1ccccc1
556:
Zipper H; Brunner H; Bernhagen J; Vitzthum F (2004).
144:
75:
463:SYBR Green I is marketed as a replacement for
605:Mackay IM; Arden KE; Nitsche A (March 2002).
8:
419:SYBR Green finds usage in several areas of
177:
18:
667:
630:
581:
363:. The SYBR family of dyes is produced by
405:
548:
379:= 497 nm) and emits green light (λ
223:
198:
173:
431:. It is also used to visualise DNA in
387:with a lower performance than ssDNA.
205:Key: CGNLCCVKSWNSDG-UHFFFAOYSA-N
119:
7:
135:
14:
683:
398:illuminated by blue light in an
307:
25:
303:(at 25 °C , 100 kPa).
50:--1-phenylquinolin-1-ium-2-yl]-
16:Dye used for molecular genetics
290:Normally supplied solvated in
1:
779:Quaternary ammonium compounds
726:10.1016/s1383-5718(98)00172-7
607:"Real-time PCR in virology"
410:Spectrogram of SYBR Green I
795:
400:epifluorescence microscope
54:-propylpropane-1,3-diamine
297:
234:
214:
189:
59:
38:
33:
24:
479:, including SYBR Green.
371:. SYBR Green I binds to
369:Thermo Fisher Scientific
774:Dimethylamino compounds
445:fluorescence microscopy
669:10.1002/cptc.202400080
562:Nucleic Acids Research
411:
403:
623:10.1093/nar/30.6.1292
482:In a study using the
409:
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365:Molecular Probes Inc.
348:) is an asymmetrical
490:Similar cyanine dyes
467:, a potential human
718:1999MRGTE.439...37S
433:gel electrophoresis
21:
574:10.1093/nar/gnh101
412:
404:
330:Infobox references
19:
706:Mutation Research
611:Nucleic Acids Res
447:. In these cases
425:molecular biology
361:molecular biology
338:Chemical compound
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292:dimethylsulfoxide
158:CompTox Dashboard
101:Interactive image
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429:quantitative PCR
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242:Chemical formula
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617:(6): 1292–305.
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512:Thiazole orange
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367:, now owned by
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20:SYBR Green I
759:Cyanine dyes
712:(1): 37–47.
709:
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614:
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568:(12): e103.
565:
561:
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437:agarose gels
421:biochemistry
418:
354:nucleic acid
345:
342:SYBR Green I
341:
340:
60:Identifiers
51:
47:
43:
350:cyanine dye
235:Properties
77:163795-75-3
769:Quinolines
748:Categories
543:References
526:Chai Green
523:Safe-Green
477:carcinogen
352:used as a
286:Solubility
265:Molar mass
88:3D model (
67:CAS Number
46:-dimethyl-
40:IUPAC name
690:CC BY 4.0
678:2367-0932
507:SYBR Gold
502:SYBR Safe
496:PicoGreen
484:Ames test
734:10029672
692:license.
641:11884626
592:15249599
537:GelGreen
531:See also
259:S
146:10436340
714:Bibcode
469:mutagen
396:cuvette
323:what is
321: (
133:PubChem
732:
676:
639:
632:101343
629:
590:
583:484200
580:
459:Safety
318:verify
315:
271:
269:509.73
219:SMILES
112:ChEMBL
34:Names
449:RNase
357:stain
194:InChI
90:JSmol
44:N',N'
730:PMID
674:ISSN
637:PMID
588:PMID
520:(YO)
514:(TO)
498:(PG)
443:and
423:and
415:Uses
722:doi
710:439
664:doi
627:PMC
619:doi
578:PMC
570:doi
385:RNA
381:max
377:max
373:DNA
359:in
278:mol
163:EPA
136:CID
750::
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346:SG
253:37
249:32
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621::
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572::
344:(
313:N
276:/
274:g
257:4
255:N
251:H
247:C
165:)
161:(
92:)
52:N
48:N
Text is available under the Creative Commons Attribution-ShareAlike License. Additional terms may apply.
