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beads coated with silica or carboxyl groups. When the beads are resuspended in solutions with high concentrations of polyethylene glycol and salts, they are capable of binding reversibly to nucleic acids. This binding is size selective, in that longer polymers of nucleic acids bind more efficiently
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Oberacker, Phil; Stepper, Peter; Bond, Donna M.; Höhn, Sven; Focken, Jule; Meyer, Vivien; Schelle, Luca; Sugrue, Victoria J.; Jeunen, Gert-Jan; Moser, Tim; Hore, Steven R. (2019-01-10).
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SPRI beads in a solution of polyethylene glycol and sodium chloride are mixed with a sample of nucleic acids. The nucleic acids bind to the beads.
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The beads are removed from the magnetic field and resuspended in water or an elution buffer, which releases the nucleic acids from the beads.
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The solution, which now contains the purified nucleic acids, is removed and used for downstream applications.
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196:"Bio-On-Magnetic-Beads (BOMB): Open platform for high-throughput nucleic acid extraction and manipulation"
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The mixture is placed in a magnetic field, which separates the nucleic-acid bound beads from the solution.
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The mixture is once again placed in a magnetic field, separating the beads from the solution.
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than shorter ones. A SPRI purification typically includes the following steps:
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and a salt. A common application of SPRI technology is purifying samples of
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139:"Solid-phase reversible immobilization for the isolation of PCR products"
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The solution is removed and the beads are washed multiple times with 80%
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beads, which reversibly bind to nucleic acids in the presence of
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The beads are allowed to dry to remove residual ethanol.
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DeAngelis, M M; Wang, D G; Hawkins, T L (1995-11-25).
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17:Solid-phase reversible immobilization
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59:Use in nucleic acid purification
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213:10.1371/journal.pbio.3000107
23:, is a method of purifying
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27:from solution. It uses
155:10.1093/nar/23.22.4742
143:Nucleic Acids Research
115:Solid-phase extraction
105:Nucleic acid methods
41:polyethylene glycol
149:(22): 4742–4743.
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206:(1): e3000107.
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63:SPRI beads are
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110:DNA sequencing
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47:amplified by
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65:paramagnetic
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55:reactions:.
37:paramagnetic
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121:References
53:sequencing
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163:0305-1048
82:in water.
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99:See also
35:-coated
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80:ethanol
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29:silica
31:- or
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177:PMID
159:ISSN
51:for
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151:doi
49:PCR
45:DNA
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