170:
to function, so if the amount of EDTA is too high it can affect the PCR. There is a version of TE buffer with 10 times less amount of EDTA that is very frequently used for the forensic STR kits. Due to the use of kits with multiplex amplification, it is necessary to have a lower amount of EDTA in the
138:
Genomic and plasmid DNA can be stored in TE Buffer at 4 °C (39.2 °F) for short-term use, or -20 °C (-4 °F) to -80 °C (-112 °F) for long-term storage. Repeated freeze-thaw cycles should be avoided.
106:
buffer, 1 ml of 1 M Tris base (pH 10–11) and 0.2 ml EDTA (0.5 M) are mixed and made up with double distilled water up to 100ml. Add microliter amounts of high molarity HCl to lower the pH to 8.
125:. The respective DNA and RNA nucleases are supposed to be less active at these pH values, but pH 8.0 can safely be used for storage of both DNA and RNA .
229:
Yagi N, Satonaka K, Horio M, Shimogaki H, Tokuda Y, Maeda S (1996). "The role of DNase and EDTA on DNA degradation in formaldehyde fixed tissues".
175:
present in the reaction. If regular TE buffer is used to dilute the sample, an imbalance will be observed in the DNA profile, while
54:
340:
160:
266:"Repeated freezing and thawing of peripheral blood and DNA in suspension: effects on DNA yield and integrity"
218:
ph7.4 TE buffer=100mM/L Tris(pH7.4)+10mM/L EDTA(pH8.0) from
Molecular Cloning: A Laboratory Manual
295:
246:
57:. The purpose of TE buffer is to solubilize DNA or RNA, while protecting it from degradation.
27:
285:
277:
238:
209:
are often used in procedures involving nucleic acids, the most common being electrophoresis.
23:
290:
265:
334:
199:, lithium borate buffer, a similar buffer containing lithium ions in place of Tris
98:
buffer, which can be read as "T ten E one buffer". To make a 100 ml solution of T
242:
206:
202:
163:
196:
172:
167:
156:
148:
47:
299:
250:
281:
110:
152:
50:
316:
132:
128:
84:
80:
70:
43:
39:
122:
118:
74:
35:
31:
114:
135:, by binding to metal cations required by this enzyme.
187:
is composed of 10 mM Tris-HCl (pH 8.0) + 0.1 mM EDTA.
65:A typical recipe for making 1X TE buffer is:
8:
147:The operation of the TE buffer is based on
289:
16:Buffer solution used in molecular biology
221:
171:sample so as not to interfere with the
38:. "TE" is derived from its components:
30:, especially in procedures involving
7:
14:
179:will have a better balance. The
231:Biotechnic & Histochemistry
117:is usually adjusted to 7.5 for
1:
90:TE buffer is also known as T
264:Ross; Haites, Kelly (1990).
113:studies from the 1980s, the
270:Journal of Medical Genetics
357:
159:. The problem is that the
42:, a common pH buffer, and
243:10.3109/10520299609117148
317:"OpenWetWare: TE buffer"
83:, bring to pH 8.0 with
73:, bring to pH 8.0 with
143:Low TE or TE Low EDTA
282:10.1136/jmg.27.9.569
131:further inactivates
22:is a commonly used
185:TE Low EDTA buffer
46:, a molecule that
28:molecular biology
348:
341:Buffer solutions
327:
325:
323:
304:
303:
293:
261:
255:
254:
226:
356:
355:
351:
350:
349:
347:
346:
345:
331:
330:
321:
319:
315:
312:
307:
263:
262:
258:
228:
227:
223:
216:
193:
145:
105:
101:
97:
93:
63:
24:buffer solution
17:
12:
11:
5:
354:
352:
344:
343:
333:
332:
329:
328:
311:
310:External links
308:
306:
305:
276:(9): 569–570.
256:
237:(3): 123–129.
220:
215:
212:
211:
210:
200:
192:
189:
166:also requires
144:
141:
103:
99:
95:
91:
88:
87:
77:
62:
59:
15:
13:
10:
9:
6:
4:
3:
2:
353:
342:
339:
338:
336:
318:
314:
313:
309:
301:
297:
292:
287:
283:
279:
275:
271:
267:
260:
257:
252:
248:
244:
240:
236:
232:
225:
222:
219:
213:
208:
204:
201:
198:
195:
194:
190:
188:
186:
182:
181:Low TE buffer
178:
174:
169:
165:
162:
158:
154:
150:
142:
140:
136:
134:
130:
126:
124:
120:
116:
112:
107:
86:
82:
78:
76:
72:
68:
67:
66:
60:
58:
56:
52:
49:
45:
41:
37:
33:
29:
25:
21:
320:. Retrieved
273:
269:
259:
234:
230:
224:
217:
184:
180:
176:
146:
137:
127:
121:and 8.0 for
108:
89:
64:
19:
18:
214:References
207:TBE buffer
203:TAE buffer
164:polymerase
34:, cDNA or
197:LB buffer
149:chelating
109:Based on
20:TE buffer
335:Category
191:See also
155:such as
111:nuclease
48:chelates
322:July 2,
300:2231649
291:1017219
251:8724437
153:cations
51:cations
298:
288:
249:
177:Low TE
151:metal
69:10 mM
61:Recipe
133:DNase
79:1 mM
53:like
324:2006
296:PMID
247:PMID
205:and
129:EDTA
85:NaOH
81:EDTA
71:Tris
44:EDTA
40:Tris
286:PMC
278:doi
239:doi
183:or
161:PCR
123:DNA
119:RNA
75:HCl
36:RNA
32:DNA
26:in
337::
294:.
284:.
274:27
272:.
268:.
245:.
235:71
233:.
173:Mg
168:Mg
157:Mg
115:pH
100:10
92:10
55:Mg
326:.
302:.
280::
253:.
241::
104:1
102:E
96:1
94:E
Text is available under the Creative Commons Attribution-ShareAlike License. Additional terms may apply.