311:
U-937V is a variant cell line derived from the original U-937 cells, the difference being their increased susceptibility to the effects of TNFα. The unique feature of these cells is the absence of cellular disintegration as an accompanying phenomenon of
248:. It should be taken into account, however, that both of said cell lines offer distinct results following the culture. U-937-derived macrophages preferentially respond to M2-inducing stimuli, subsequently leading them to acquire the phenotype of
256:. Both cell lines can be artificially polarized in either direction though. The only difference being that the M1 phenotype establishment in U-937-derived macrophages will not be as strong as in THP-1, and vice versa.
555:
Parenti S, Sandoni L, Montanari M, Zanocco-Marani T, Anesi A, Iotti S, et al. (August 2021). "Magnesium favors the capacity of vitamin D3 to induce the monocyte differentiation of U937 cells".
272:
activated mutation observed in about 10% of AML patients. In this study, they also confirm that the U-937 cells do respond to a model JAK3 inhibitor, and their proliferation is thus impaired by it.
34:
of a 37-year-old male patient in 1974. Due to the relative uniformity of expanded cultures and lower maintenance requirements these cells have been since used as an important tool for studying
590:
92:
production in U937 cells. This cell line is therefore widely used for cytokine level read-outs as a result of exposure to various chemical compounds. Namely, production of
85:
471:
Lehmann MH (June 1998). "Recombinant human granulocyte-macrophage colony-stimulating factor triggers interleukin-10 expression in the monocytic cell line U937".
332:
release) following the induction of apoptosis are not drastically changed in these cells. This subtype could therefore serve as a good model for regulation of
508:"Sampling of major histocompatibility complex class I-associated peptidome suggests relatively looser global association of HLA-B*5101 with peptides"
41:
and different kinds of cell-to-cell interactions. U-937 cells mature and differentiate in response to a number of soluble stimuli, adopting the
441:
117:
206:
209:(12-O-tetradecanoylphorbol13-acetate). Further differentiation into dendritic cells can be induced by exposing them to self-peptide from
300:
249:
293:
194:
368:
Sundström C, Nilsson K (May 1976). "Establishment and characterization of a human histiocytic lymphoma cell line (U-937)".
213:(Ep1.B). This treatment leads to changes in surface marker expression and thus induces acquirement of DC-like morphology.
424:. In Verhoeckx K, Cotter P, López-Expósito I, Kleiveland C, Lea T, Mackie A, Requena T, Swiatecka D, Wichers H (eds.).
821:
224:
and remodeling. This phenotype can be achieved by combining stimulation with PMA and vitamin D3 with addition of
128:
38:
193:
Induction of differentiation of U-937 into monocytes or macrophages can be achieved by various agents such as
285:
253:
140:
265:
584:
289:
157:
for up to 3 months or stored frozen in -80 °C for several years following an appropriate protocol.
113:
109:
237:
42:
337:
24:
143:
alleles present in U-937 cells are HLA-A*03:01, A*31:01, B*18:01, B*51:01, Cw*01:02 and Cw*07:02.
393:
321:
166:
93:
755:
Stasiłojć G, Pinto S, Wyszkowska R, Wejda M, Słomińska EM, Filipska M, et al. (June 2013).
264:
According to Si et al. (2022), the U-937 cell line specifically could be a promising model for
786:
737:
686:
635:
604:
Nascimento CR, Rodrigues
Fernandes NA, Gonzalez Maldonado LA, Rossa Junior C (December 2022).
572:
537:
488:
447:
437:
385:
606:"Comparison of monocytic cell lines U937 and THP-1 as macrophage models for in vitro studies"
776:
768:
727:
717:
676:
666:
655:"Identification of U937 Acute Myeloid Leukemia Cells as a Sensitive Model to JAK3 Inhibitor"
625:
617:
564:
527:
519:
480:
429:
377:
210:
292:. Prasad et al. (2020) present the current possibilities of ROS imaging in these cells via
241:
221:
202:
781:
756:
732:
705:
681:
654:
630:
605:
532:
507:
333:
269:
89:
54:
484:
815:
198:
101:
97:
73:
397:
329:
296:
174:
105:
61:
704:
Prasad A, Sedlářová M, Balukova A, Ovsii A, Rác M, Křupka M, et al. (2020).
523:
433:
621:
252:. Whereas, in the case of THP-1 cells, the polarization is reversed in favor of
28:
421:
772:
722:
217:
216:
Beside the cell types mentioned above, U-937 cells can be differentiated into
178:
170:
69:
50:
671:
313:
281:
225:
158:
81:
65:
35:
20:
790:
741:
690:
639:
576:
541:
451:
381:
356:
284:. One of the traits that are especially worth studying is their ability of
280:
As stated before, U-937 is a valuable tool for broadening the knowledge of
568:
492:
389:
153:
132:
121:
46:
31:
757:"U937 variant cells as a model of apoptosis without cell disintegration"
806:
325:
316:. It was documented that the basic molecular processes (degradation of
136:
77:
426:
The Impact of Food
Bioactives on Health: in vitro and ex vivo models
182:
162:
317:
653:
Si H, Wang J, He R, Yu X, Li S, Huang J, et al. (2021).
177:). In some cases, another supplement that can be utilized is
228:, which can further perpetuate the differentiation process.
506:
Gebreselassie D, Spiegel H, Vukmanovic S (November 2006).
244:
cells, is a crucial tool for studying these phagocytes
288:(reactive oxygen species) generation as a part of
589:: CS1 maint: DOI inactive as of September 2024 (
706:"Reactive Oxygen Species Imaging in U937 Cells"
165:media can be used, both supplemented with 10%
8:
236:Due to limited possibilities for obtaining
64:lineage and thus secrete a large number of
268:, as it was shown to actively express the
780:
731:
721:
680:
670:
629:
531:
428:. Cham (CH): Springer. pp. 147–159.
131:causing a fusion of MLLT10 and Ap-3-like
761:Cellular & Molecular Biology Letters
357:https://www.atcc.org/products/crl-1593.2
420:Chanput W, Peters V, Wichers H (2015).
348:
127:U-937 cells bear the t(10;11)(p13;q14)
582:
232:Special features & use in research
80:) or in response to soluble stimuli.
7:
415:
413:
411:
409:
407:
610:Biochemistry and Biophysics Reports
250:alternatively activated macrophages
197:(phorbol 12-myristate 13-acetate),
301:confocal laser scanning microscopy
88:for example independently promote
14:
147:Maintenance & differentiation
370:International Journal of Cancer
294:electron paramagnetic resonance
205:(1,25- dihydroxyvitamin D3) or
240:, U-937 cell line, beside the
27:. They were isolated from the
1:
485:10.1016/S0161-5890(98)00043-1
72:either constitutively (e.g.,
524:10.1016/j.humimm.2006.08.294
434:10.1007/978-3-319-16104-4_14
290:non-specific immune response
622:10.1016/j.bbrep.2022.101383
220:– cell subtype crucial for
151:U-937 line can be cultured
838:
807:Cellosaurus entry for U937
19:are a pro-monocytic model
773:10.2478/s11658-013-0087-y
723:10.3389/fphys.2020.552569
189:Differentiation induction
169:and antibiotics (usually
672:10.3389/fonc.2021.807200
254:classical M1 macrophages
710:Frontiers in Physiology
571:(inactive 2024-09-13).
355:ATCC U-937 CRL-1593.2™
124:is commonly measured.
45:and characteristics of
422:"THP-1 and U937 Cells"
382:10.1002/ijc.2910170504
266:acute myeloid leukemia
659:Frontiers in Oncology
569:10.1684/mrh.2021.0490
473:Molecular Immunology
238:primary macrophages
161:or, alternatively,
60:U-937 cells are of
25:biomedical research
557:Magnesium Research
443:978-3-319-15791-7
324:externalization,
135:assembly protein
829:
822:Human cell lines
795:
794:
784:
752:
746:
745:
735:
725:
701:
695:
694:
684:
674:
650:
644:
643:
633:
601:
595:
594:
588:
580:
552:
546:
545:
535:
512:Human Immunology
503:
497:
496:
468:
462:
461:
459:
458:
417:
402:
401:
365:
359:
353:
211:apolipoprotein E
84:and recombinant
837:
836:
832:
831:
830:
828:
827:
826:
812:
811:
803:
798:
754:
753:
749:
703:
702:
698:
652:
651:
647:
603:
602:
598:
581:
554:
553:
549:
518:(11): 894–906.
505:
504:
500:
470:
469:
465:
456:
454:
444:
419:
418:
405:
367:
366:
362:
354:
350:
346:
309:
278:
262:
234:
222:bone resorption
191:
149:
55:dendritic cells
39:differentiation
12:
11:
5:
835:
833:
825:
824:
814:
813:
810:
809:
802:
801:External links
799:
797:
796:
767:(2): 249–262.
747:
696:
645:
596:
563:(3): 114–129.
547:
498:
479:(8): 479–485.
463:
442:
403:
376:(5): 565–577.
360:
347:
345:
342:
334:apoptotic body
308:
305:
277:
274:
261:
258:
233:
230:
199:retinoic acids
190:
187:
148:
145:
13:
10:
9:
6:
4:
3:
2:
834:
823:
820:
819:
817:
808:
805:
804:
800:
792:
788:
783:
778:
774:
770:
766:
762:
758:
751:
748:
743:
739:
734:
729:
724:
719:
715:
711:
707:
700:
697:
692:
688:
683:
678:
673:
668:
664:
660:
656:
649:
646:
641:
637:
632:
627:
623:
619:
615:
611:
607:
600:
597:
592:
586:
578:
574:
570:
566:
562:
558:
551:
548:
543:
539:
534:
529:
525:
521:
517:
513:
509:
502:
499:
494:
490:
486:
482:
478:
474:
467:
464:
453:
449:
445:
439:
435:
431:
427:
423:
416:
414:
412:
410:
408:
404:
399:
395:
391:
387:
383:
379:
375:
371:
364:
361:
358:
352:
349:
343:
341:
339:
336:formation or
335:
331:
327:
323:
319:
315:
306:
304:
302:
298:
295:
291:
287:
283:
275:
273:
271:
267:
259:
257:
255:
251:
247:
243:
239:
231:
229:
227:
223:
219:
214:
212:
208:
204:
200:
196:
188:
186:
184:
180:
176:
172:
168:
164:
160:
156:
155:
146:
144:
142:
138:
134:
130:
129:translocation
125:
123:
119:
115:
111:
107:
103:
99:
95:
91:
87:
83:
79:
75:
71:
67:
63:
58:
56:
52:
48:
44:
40:
37:
33:
30:
26:
22:
18:
764:
760:
750:
713:
709:
699:
662:
658:
648:
613:
609:
599:
585:cite journal
560:
556:
550:
515:
511:
501:
476:
472:
466:
455:. Retrieved
425:
373:
369:
363:
351:
328:activity or
310:
297:spectroscopy
279:
276:Phagocytosis
263:
245:
235:
215:
192:
179:zwitterionic
175:streptomycin
152:
150:
126:
59:
16:
15:
218:osteoclasts
51:macrophages
29:histiocytic
17:U-937 cells
716:: 552569.
665:: 807200.
616:: 101383.
457:2023-01-07
344:References
282:phagocytes
203:vitamin D3
171:penicillin
70:chemokines
43:morphology
314:apoptosis
307:Apoptosis
226:magnesium
181:buffer –
159:RPMI 1640
66:cytokines
47:monocytes
36:phagocyte
21:cell line
816:Category
791:23605997
742:33178031
691:35111683
640:36420419
577:34859787
542:17145369
452:29787063
398:19334036
338:blebbing
260:Leukemia
246:in vitro
154:in vitro
133:clathrin
32:lymphoma
23:used in
782:6275624
733:7593787
682:8802890
631:9677084
533:2269730
493:9798652
326:caspase
62:myeloid
789:
779:
740:
730:
689:
679:
638:
628:
575:
540:
530:
491:
450:
440:
396:
390:178611
388:
139:. The
137:PICALM
86:GM-CSF
78:GM-CSF
394:S2CID
242:THP-1
183:HEPES
122:NF-κB
118:COX-2
110:TNF-α
106:IL-10
94:IL-1β
90:IL-10
787:PMID
738:PMID
687:PMID
636:PMID
591:link
573:PMID
538:PMID
489:PMID
448:PMID
438:ISBN
386:PMID
330:CytC
299:and
270:JAK3
163:DMEM
120:and
114:iNOS
102:IL-8
98:IL-6
82:TNFα
76:and
74:IL-1
68:and
777:PMC
769:doi
728:PMC
718:doi
677:PMC
667:doi
626:PMC
618:doi
565:doi
528:PMC
520:doi
481:doi
430:doi
378:doi
318:DNA
286:ROS
207:TPA
195:PMA
173:or
167:FBS
141:HLA
53:or
818::
785:.
775:.
765:18
763:.
759:.
736:.
726:.
714:11
712:.
708:.
685:.
675:.
663:11
661:.
657:.
634:.
624:.
614:32
612:.
608:.
587:}}
583:{{
561:34
559:.
536:.
526:.
516:67
514:.
510:.
487:.
477:35
475:.
446:.
436:.
406:^
392:.
384:.
374:17
372:.
340:.
322:PS
320:,
303:.
201:,
185:.
116:,
112:,
108:,
104:,
100:,
96:,
57:.
49:,
793:.
771::
744:.
720::
693:.
669::
642:.
620::
593:)
579:.
567::
544:.
522::
495:.
483::
460:.
432::
400:.
380::
Text is available under the Creative Commons Attribution-ShareAlike License. Additional terms may apply.