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46:. This test helps to identify the type of jaundice. The serum of the patient is mixed with diazo reagent. If a red colour develops immediately it is called a direct positive. It happens if conjugated bilirubin is present. In an indirect positive test, the patient's serum is first treated with alcohol and later mixed with diazo reagent. This causes development of a red colour. It is seen if unconjugated bilirubin is present. If both conjugated and unconjugated bilirubin are present the reaction is termed a biphasic reaction.
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levels in blood. More specifically, it determines the amount of conjugated bilirubin in the blood. The reaction produces
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to produce purple coloured azobilirubin. This reaction is highly useful in understanding the nature of
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Chemical reaction used to measure bilirubin levels in blood
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30:. Principle: bilirubin reacts with diazotised
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38:. This was pioneered by the Dutch physician,
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97:Gray, C.H.; Whidborne, J. (6 October 1945).
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148:"Bilirubin and the van den Bergh reaction"
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65:"Chemistry of the van den Bergh reaction"
99:"Studies of the Van den Bergh reaction"
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22:is a chemical reaction used to measure
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40:Abraham Albert Hijmans van den Bergh
200:. You can help Knowledge (XXG) by
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72:Journal of Biological Chemistry
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84:10.1016/S0021-9258(18)73401-7
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20:Van den Bergh reaction
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