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For tissues that are not directly acidic or basic, it can be difficult to use only one stain to reveal the necessary structures of interest. A combination of the three different stains in precise amounts applied in the correct order reveals the details selectively. This is the result of more than
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The primary application when the stain was introduced was differentiation of structures in connective tissue, and this remains its most common use. Some work however has indicated the stain can highlight differential RNA synthesis. This has been used in identifying ectopic
88:. Many variants of the method exist to simplify or speed processing or to stain other materials. Mallory's and other polychrome stains developed in the early 20th century led to
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just electrostatic interactions of stain with the tissue and the stain not being washed out after each step. Collectively the stains complement one another.
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Wołuń-Cholewa, Maria; Szymanowski, Krzysztof; Andrusiewicz, Mirosław; Szczerba, Anna; Warchoł, Jerzy B. (2010).
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219:"Trichrome Mallory's stain may indicate differential rates of RNA synthesis in eutopic and ectopic endometrium"
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Routine
Cytological Staining Techniques: Theoretical Background and Practice
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73:. It is used in examining the collagen of connective tissue.
125:. Philadelphia: Lippincott Williams & Wilkins. pp.
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The staining technique was first published in 1900 by
61:. As a result, this staining technique can reveal
49:that make up the cell. It uses the three stains:
202:Boon, Mathilde E.; Drijver, Johanna S. (1986).
92:and other popular polychrome staining methods.
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30:Uterus of a rat stained with Mallory's stain.
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152:"A Contribution to Staining Methods"
223:Folia Histochemica et Cytobiologica
278:. You can help Knowledge (XXG) by
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86:Harvard University Medical School
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156:Journal of Experimental Medicine
45:to aid in revealing different
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121:Histology: A Text and Atlas
16:Stain utilized in histology
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236:10.2478/v10042-008-0106-4
117:Ross, Michael H. (2011).
35:Mallory's trichrome stain
270:This article related to
84:, then a histologist at
21:Masson's trichrome stain
19:Not to be confused with
41:is a stain utilized in
150:Mallory, F.B. (1900).
39:Mallory's Triple Stain
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168:10.1084/jem.5.1.15
90:Papanicolaou stain
82:Frank Burr Mallory
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162:(1): 15–20.
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55:acid fuchsin
51:aniline blue
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37:also called
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206:. Elsevier.
98:endometrial
65:, ordinary
319:Categories
104:References
325:Histology
272:pathology
67:cytoplasm
43:histology
245:20529831
186:19866932
100:tissue.
63:collagen
59:orange G
177:2117995
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69:, and
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274:is a
276:stub
241:PMID
182:PMID
131:ISBN
129:–6.
231:doi
172:PMC
164:doi
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