803:
protein biosensors and actuators, once it passes the initial threshold of practicality, there will be more attempts to reshape the tool for its usage in different target applications, each with a different emphasis and requirement for a subset of performance metrics. For example, authors of JEDI-2P stated that the negative-going (bright-to-dim) sensor is good for detecting subthreshold depolarizations and hyperpolarizations but positive-going (dim-to-bright) sensors might be better for spike detection. We may argue that it takes effort to engineer (screen) a perfect sensor, but often the more compelling reason is that simply there is not a unanimous definition of such perfection. For example, scientist might prefer sensors of different emission and excitation colors to be spectrally compatible with other
3493:
774:. It has subcellular spatial resolution and temporal resolution as low as 0.2 milliseconds, about an order of magnitude faster than calcium imaging. This allows for spike detection fidelity comparable to electrode-based electrophysiology but without the invasiveness. Researchers have used it to probe neural communications of an intact brain (of
86:
GEVI can have many configuration designs in order to realize voltage sensing function. An essential feature of GEVI structure is that it must situate on the cell membrane. Conceptually, the structure of a GEVI should permit the function of sensing the voltage difference and reporting it by change in
58:
Despite that the idea of optical measurement of neuronal activity was proposed in the late 1960s, the first successful GEVI that was convenient enough to put into actual use was not developed until technologies of genetic engineering had become mature in the late 1990s. The first GEVI, coined FlaSh,
802:
For GEVI development, its future direction is highly coupled with the target applications. With newer generations of GEVIs overcome the poor performance of the first generation ones, we will see more routes open up for GEVIs to be used in more challenging and versatile applications. Like many other
1287:
Piatkevich, Kiryl D.; Jung, Erica E.; Straub, Christoph; Linghu, Changyang; Park, Demian; Suk, Ho-Jun; Hochbaum, Daniel R.; Goodwin, Daniel; Pnevmatikakis, Eftychios; Pak, Nikita; Kawashima, Takashi; Yang, Chao-Tsung; Rhoades, Jeffrey L.; Shemesh, Or; Asano, Shoh; Yoon, Young-Gyu; Freifeld, Limor;
1005:
Piatkevich, Kiryl D.; Jung, Erica E.; Straub, Christoph; Linghu, Changyang; Park, Demian; Suk, Ho-Jun; Hochbaum, Daniel R.; Goodwin, Daniel; Pnevmatikakis, Eftychios; Pak, Nikita; Kawashima, Takashi; Yang, Chao-Tsung; Rhoades, Jeffrey L.; Shemesh, Or; Asano, Shoh; Yoon, Young-Gyu; Freifeld, Limor;
90:
By structure, GEVIs can be classified into four categories based on the current findings: (1) GEVIs contain a fluorescent protein FRET pair, e.g. VSFP1, (2) Single opsin GEVIs, e.g. Arch, (3) Opsin-FP FRET pair GEVIs, e.g. MacQ-mCitrine, (4) single FP with special types of voltage sensing domains,
1063:
Piatkevich, Kiryl D.; Bensussen, Seth; Tseng, Hua-an; Shroff, Sanaya N.; Lopez-Huerta, Violeta
Gisselle; Park, Demian; Jung, Erica E.; Shemesh, Or A.; Straub, Christoph; Gritton, Howard J.; Romano, Michael F.; Costa, Emma; Sabatini, Bernardo L.; Fu, Zhanyan; Boyden, Edward S.; Han, Xue (October
104:
survey of the organism. Some GEVIs might have similar components, but with different positioning of them. For example, ASAP1 and ArcLight both use a VSD and one FP, but the FP of ASAP1 is on the outside of the cell whereas that of ArcLight is on the inside, and the two FPs of VSFP-Butterfly are
67:). Unlike fluorescent proteins, the discovery of new GEVIs were seldom inspired by the nature, for it is hard to find an organism which naturally has the ability to change its fluorescence based on voltage. Therefore, new GEVIs are mostly the products of genetic and protein engineering.
3017:
Liu, Zhuohe; Lu, Xiaoyu; Villette, Vincent; Gou, Yueyang; Colbert, Kevin L.; Lai, Shujuan; Guan, Sihui; Land, Michelle A.; Lee, Jihwan; Assefa, Tensae; Zollinger, Daniel R.; Korympidou, Maria M.; Vlasits, Anna L.; Pang, Michelle M.; Su, Sharon (2022-08-18).
87:
fluorescence. Usually, the voltage-sensing domain (VSD) of a GEVI spans across the membrane, and is connected to the fluorescent protein(s). However, it is not necessary that sensing and reporting should happen in different structures, e.g. Archons.
955:
Villette, V; Chavarha, M; Dimov, IK; Bradley, J; Pradhan, L; Mathieu, B; Evans, SW; Chamberland, S; Shi, D; Yang, R; Kim, BB; Ayon, A; Jalil, A; St-Pierre, F; Schnitzer, MJ; Bi, G; Toth, K; Ding, J; Dieudonné, S; Lin, MZ (12 December 2019).
754:), plasma membrane localization, adaptability of deep-tissue imaging, etc. For now, no existing GEVI meets all the desired properties, so searching for a perfect GEVI is still a quite competitive research area.
1288:
Saulnier, Jessica L.; Riegler, Clemens; Engert, Florian; Hughes, Thom; Drobizhev, Mikhail; Szabo, Balint; Ahrens, Misha B.; Flavell, Steven W.; Sabatini, Bernardo L.; Boyden, Edward S. (April 2018).
1006:
Saulnier, Jessica L.; Riegler, Clemens; Engert, Florian; Hughes, Thom; Drobizhev, Mikhail; Szabo, Balint; Ahrens, Misha B.; Flavell, Steven W.; Sabatini, Bernardo L.; Boyden, Edward S. (April 2018).
2985:"We fused paQuasAr3 with a trafficking motif from the soma-localized KV2.1 potassium channel, which led to largely soma-localized expression (Fig. 2a, b). We called this construct paQuasAr3-s."
746:
A GEVI can be evaluated by its many characteristics. These traits can be classified into two categories: performance and compatibility. The performance properties include brightness,
3307:
Zhang, Joe Z.; Termglinchan, Vittavat; Shao, Ning-Yi; Itzhaki, Ilanit; Liu, Chun; Ma, Ning; Tian, Lei; Wang, Vicky Y.; Chang, Alex C. Y.; Guo, Hongchao; Kitani, Tomoya (2019-05-02).
762:
Different types of GEVIs are being developed in many biological or physiological research areas. It is thought to be superior to conventional voltage detecting methods like
2826:
Chamberland, S; Yang, HH; Pan, MM; Evans, SW; Guan, S; Chavarha, M; Yang, Y; Salesse, C; Wu, H; Wu, JC; Clandinin, TR; Toth, K; Lin, MZ; St-Pierre, F (27 July 2017).
50:
signals from cultured cells, live animals, and ultimately human brain. Examples of notable GEVIs include ArcLight, ASAP1, ASAP3, Archons, SomArchon, and Ace2N-mNeon.
78:. The former method contributes to the most of new GEVI variants, but recent researches using directed evolution have shown promising results in GEVI optimization.
807:. Recently, to compensate for the low signal-to-noise ratio (SNR) due to the poor brightness of GEVI, several denoising methods have been applied to increase SNR.
3261:
1661:
Baker BJ, Lee H, Pieribone VA, et al. (2007). "Three fluorescent protein voltage sensors exhibit low plasma membrane expression in mammalian cells".
1915:
Kralj JM, Hochbaum DR, Douglass AD, et al. (2011). "Electrical spiking in
Escherichia coli probed with a fluorescent voltage-indicating protein".
1347:"Directed Evolution of Key Residues in Fluorescent Protein Inverses the Polarity of Voltage Sensitivity in the Genetically Encoded Indicator ArcLight"
3457:
1498:
Sakai R, Repunte-Canonigo V, Raj CD, et al. (2001). "Design and characterization of a DNA-encoded, voltage-sensitive fluorescent protein".
3383:
1970:"Genetically encoded fluorescent voltage sensors using the voltage-sensing domain of Nematostella and Danio phosphatases exhibit fast kinetics"
3309:"A Human iPSC Double-Reporter System Enables Purification of Cardiac Lineage Subpopulations with Distinct Function and Drug Response Profiles"
2611:"Pado, a fluorescent protein with proton channel activity can optically monitor membrane potential, intracellular pH, and map gap junctions"
1500:
1767:
Tsutsui H, Karasawa S, Okamura Y, et al. (2008). "Improving membrane voltage measurements using FRET with new fluorescent proteins".
438:
418:
398:
323:
248:
194:
157:
3221:
1401:
3447:
3410:
1445:
Murata Y, Iwasaki H, Sasaki M, et al. (2005). "Phosphoinositide phosphatase activity coupled to an intrinsic voltage sensor".
3219:
Knöpfel T, Gallero-Salas Y, Song C (2015). "Genetically encoded voltage indicators for large scale cortical imaging come of age".
2182:
Akemann W, Mutoh H, Perron A, et al. (2012). "Imaging neural circuit dynamics with a voltage-sensitive fluorescent protein".
1974:
1663:
3000:
823:
97:
3529:
2720:"A Bright and Fast Red Fluorescent Protein Voltage Indicator That Reports Neuronal Activity in Organotypic Brain Slices"
2021:"Single action potentials and subthreshold electrical events imaged in neurons with a fluorescent protein voltage probe"
850:"Single action potentials and subthreshold electrical events imaged in neurons with a fluorescent protein voltage probe"
2438:"Archaerhodopsin variants with enhanced voltage-sensitive fluorescence in mammalian and Caenorhabditis elegans neurons"
3081:
2724:
1818:
1708:"Engineering of a genetically encodable fluorescent voltage sensor exploiting fast Ci-VSP voltage-sensing movements"
3534:
3376:
2184:
2282:
1351:
750:, sensitivity, kinetics (speed), linearity of response, etc., while the compatibility properties cover toxicity (
60:
2828:"Fast two-photon imaging of subcellular voltage dynamics in neuronal tissue with genetically encoded indicators"
904:"High-fidelity optical reporting of neuronal electrical activity with an ultrafast fluorescent voltage sensor"
3496:
3524:
1868:
1188:
Cohen LB, Keynes RD, Hille B (1968). "Light scattering and birefringence changes during nerve activity".
3519:
3478:
3369:
2994:
2442:
2333:
771:
763:
2278:"Improved detection of electrical activity with a voltage probe based on a voltage-sensing phosphatase"
2950:
2891:
2879:"Biophysical Characterization of Genetically Encoded Voltage Sensor ASAP1: Dynamic Range Improvement"
2782:
2623:
2512:
2450:
2341:
2232:
2136:
1926:
1721:
1615:
1556:
1456:
1199:
1142:
1077:
804:
2883:
2770:"Improving a genetically encoded voltage indicator by modifying the cytoplasmic charge composition"
2224:
1548:
908:
791:
3020:"Sustained deep-tissue voltage recording using a fast indicator evolved for two-photon microscopy"
2774:
2615:
2499:"High-speed recording of neural spikes in awake mice and flies with a fluorescent voltage sensor"
1950:
1794:
1688:
1524:
1480:
1290:"A robotic multidimensional directed evolution approach applied to fluorescent voltage reporters"
1223:
1131:"High-speed recording of neural spikes in awake mice and flies with a fluorescent voltage sensor"
1008:"A robotic multidimensional directed evolution approach applied to fluorescent voltage reporters"
620:
334:
93:
75:
31:
2558:"Single-molecule fluorimetry and gating currents inspire an improved optical voltage indicator"
3426:
3348:
3330:
3289:
3237:
3201:
3150:
3107:
3057:
3039:
2976:
2917:
2859:
2808:
2750:
2700:
2649:
2591:
2538:
2503:
2476:
2418:
2367:
2309:
2258:
2200:
2164:
2103:
2052:
2001:
1942:
1917:
1894:
1844:
1786:
1749:
1680:
1643:
1582:
1516:
1472:
1427:
1377:
1327:
1309:
1269:
1215:
1170:
1129:
Gong, Y; Huang, C; Li, JZ; Grewe, BF; Zhang, Y; Eismann, S; Schnitzer, MJ (11 December 2015).
1111:
1093:
1045:
1027:
987:
934:
881:
47:
2937:"Voltage imaging and optogenetics reveal behaviour-dependent changes in hippocampal dynamics"
1814:"Rational optimization and imaging in vivo of a genetically encoded optical voltage reporter"
3462:
3406:
3338:
3320:
3279:
3269:
3229:
3191:
3183:
3140:
3097:
3089:
3047:
3031:
2966:
2958:
2941:
2907:
2899:
2849:
2839:
2798:
2790:
2740:
2732:
2690:
2682:
2639:
2631:
2581:
2571:
2528:
2520:
2466:
2458:
2408:
2400:
2357:
2349:
2299:
2291:
2248:
2240:
2192:
2154:
2144:
2093:
2085:
2042:
2034:
2025:
1991:
1983:
1934:
1884:
1876:
1834:
1826:
1778:
1739:
1729:
1672:
1633:
1623:
1572:
1564:
1508:
1464:
1447:
1417:
1409:
1367:
1359:
1317:
1301:
1259:
1250:
1207:
1190:
1160:
1150:
1101:
1085:
1035:
1019:
977:
969:
924:
916:
871:
861:
786:
359:
105:
separated by the VSD, while the two FPs of
Mermaid are relatively close to each other.
3431:
3421:
2387:"All-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins"
767:
2123:"A fluorescent, genetically-encoded voltage probe capable of resolving action potentials"
2072:"Optical recording of action potentials in mammalian neurons using a microbial rhodopsin"
848:
Jin, L; Han, Z; Platisa, J; Wooltorton, JR; Cohen, LB; Pieribone, VA (6 September 2012).
2954:
2895:
2786:
2627:
2516:
2454:
2345:
2236:
2140:
1930:
1725:
1619:
1560:
1460:
1203:
1146:
1081:
3343:
3308:
3284:
3256:
3196:
3174:
3169:
3131:
3102:
3076:
3052:
3019:
2971:
2936:
2912:
2878:
2854:
2827:
2803:
2769:
2745:
2719:
2695:
2673:
2668:
2644:
2610:
2586:
2557:
2533:
2498:
2471:
2437:
2413:
2391:
2386:
2362:
2328:
2304:
2277:
2253:
2219:
2159:
2122:
2098:
2076:
2071:
2047:
2020:
1996:
1969:
1889:
1863:
1839:
1813:
1769:
1744:
1707:
1638:
1601:
1577:
1543:
1422:
1396:
1372:
1346:
1322:
1289:
1165:
1130:
1106:
1065:
1040:
1007:
982:
957:
929:
903:
876:
849:
747:
71:
2669:"Subcellular Imaging of Voltage and Calcium Signals Reveals Neural Processing In Vivo"
1568:
1264:
1245:
958:"Ultrafast Two-Photon Imaging of a High-Gain Voltage Indicator in Awake Behaving Mice"
3513:
3416:
1512:
1397:"The evolving capabilities of rhodopsin-based genetically encoded voltage indicators"
751:
265:
1798:
1602:"Engineering and characterization of an enhanced fluorescent protein voltage sensor"
1528:
3392:
3093:
2736:
1987:
1954:
1880:
1830:
1692:
1676:
1484:
1227:
43:
39:
1544:"A genetically targetable fluorescent probe of channel gating with rapid kinetics"
2329:"Imaging neural spiking in brain tissue using FRET-opsin protein voltage sensors"
2295:
2149:
2038:
1734:
1628:
1363:
866:
64:
3325:
3233:
3187:
3145:
3126:
3035:
2794:
2686:
2244:
1413:
973:
2962:
2903:
1305:
1089:
1023:
776:
3334:
3043:
2220:"Improving FRET Dynamic Range with Bright Green and Red Fluorescent Proteins"
1313:
1097:
1031:
2524:
1938:
1155:
3352:
3293:
3241:
3205:
3154:
3111:
3061:
2980:
2921:
2863:
2812:
2754:
2704:
2653:
2595:
2542:
2480:
2422:
2371:
2313:
2262:
2204:
2168:
2107:
2056:
2005:
1946:
1898:
1848:
1790:
1753:
1684:
1647:
1586:
1520:
1476:
1431:
1381:
1331:
1174:
1115:
1049:
991:
938:
885:
3274:
3170:"Genetically targeted optical electrophysiology in intact neural circuits"
2196:
1273:
1219:
2127:
1712:
1606:
781:
101:
2844:
2576:
1468:
2462:
2404:
2353:
2089:
1782:
35:
27:
3077:"Genetically Encoded Voltage Indicators: Opportunities and Challenges"
2635:
1211:
950:
948:
920:
100:(Ci-VSP or Ci-VSD (domain)), which was discovered in 2005 from the
2562:
3257:"Genetically Encoded Voltage Indicators in Circulation Research"
3127:"Neuronal Computations Made Visible with Subcellular Resolution"
2989:"QuasAr2(K171R)-TS-citrine-TS-TS-TS-ER2, which we call QuasAr3."
3365:
3361:
2987:"We called QuasAr3(V59A) 'photoactivated QuasAr3' (paQuasAr3)."
1066:"Population imaging of neural activity in awake behaving mice"
1246:"A genetically encoded optical probe of membrane voltage"
2070:
Kralj JM, Douglass AD, Hochbaum DR, et al. (2011).
2436:
Flytzanis NC, Bedbrook CN, Chiu H, et al. (2014).
2718:
Abdelfattah AS, Farhi SL, Zhao Y, et al. (2016).
902:
St-Pierre F, Marshall JD, Yang Y, et al. (2014).
2121:
Barnett L, Platisa J, Popovic M, et al. (2012).
3168:
Cao G, Platisa J, Pieribone VA, et al. (2013).
1864:"Red-shifted voltage-sensitive fluorescent proteins"
3471:
3440:
3399:
2385:Hochbaum DR, Zhao Y, Farhi SL, et al. (2014).
2327:Gong Y, Wagner MJ, Zhong Li J, et al. (2014).
1706:Lundby A, Mutoh H, Dimitrov D, et al. (2008).
3255:Kaestner L, Tian Q, Kaiser E, et al. (2015).
2276:Tsutsui H, Jinno Y, Tomita A, et al. (2013).
2667:Yang HH, St-Pierre F, Sun X, et al. (2016).
2218:Lam AJ, St-Pierre F, Gong Y, et al. (2013).
1862:Perron A, Mutoh H, Launey T, et al. (2009).
91:e.g. ASAP1. A majority of GEVIs are based on the
70:Two methods can be utilized to find novel GEVIs:
1345:Platisa J, Vasan G, Yang A, et al. (2017).
1600:Dimitrov D, He Y, Mutoh H, et al. (2007).
303:Modified green-absorbing proteorhodopsin (GPR)
289:RFP/YFP (Citrine, mOrange2, TagRFP, or mKate2)
2768:Lee S, Geiller T, Jung A, et al. (2017).
3377:
2019:Jin L, Han Z, Platisa J, et al. (2012).
8:
2497:Gong Y, Huang C, Li JZ, et al. (2015).
1968:Baker BJ, Jin L, Han Z, et al. (2012).
2935:Adam Y, Kim JJ, Lou S, et al. (2019).
3384:
3370:
3362:
2556:Treger JS, Priest MF, Bezanilla F (2015).
107:
3342:
3324:
3283:
3273:
3195:
3144:
3101:
3051:
2970:
2911:
2853:
2843:
2802:
2744:
2694:
2643:
2585:
2575:
2532:
2470:
2412:
2361:
2303:
2252:
2158:
2148:
2097:
2046:
1995:
1888:
1838:
1743:
1733:
1637:
1627:
1576:
1421:
1371:
1321:
1263:
1164:
1154:
1105:
1039:
981:
928:
875:
865:
824:"Genetically-Encoded Voltage Indicators"
815:
736:Names in italic denote GEVIs not named.
326:pair: CFP (Cerulean) and YFP (Citrine)
251:pair: Marine GFP (mUKG) and OFP (mKOκ)
197:pair: CFP (Cerulean) and YFP (Citrine)
2992:
401:pair: YFP (mCitrine) and RFP (mKate2)
46:recording tool that enables exporting
3012:
3010:
2492:
2490:
1910:
1908:
687:FRET doner: Janelia Fluor (chemical)
59:was constructed by fusing a modified
20:Genetically encoded voltage indicator
7:
3492:
1239:
1237:
897:
895:
63:with a voltage-sensitive K channel (
421:pair: GFP (Clover) and RFP(mRuby2)
109:Table of GEVIs and their structure
34:in a cell and relate the change in
460:FRET doner: mCitrine, or mOrange2
14:
780:or mouse), electrical spiking of
766:electrophysiological recordings,
617:Modified super ecliptic pHluorin
600:Circularly permuted RFP (mApple)
549:Modified super ecliptic pHluorin
345:Modified super ecliptic pHluorin
3491:
1812:Sjulson L, Miesenböck G (2008).
1513:10.1046/j.0953-816x.2001.01617.x
3125:Kaschula R, Salecker I (2016).
790:), and human stem-cell derived
3094:10.1523/JNEUROSCI.1095-16.2016
2737:10.1523/JNEUROSCI.3484-15.2016
1988:10.1016/j.jneumeth.2012.05.016
1881:10.1016/j.chembiol.2009.11.014
1831:10.1523/JNEUROSCI.0055-08.2008
1677:10.1016/j.jneumeth.2006.10.005
1542:Ataka K, Pieribone VA (2002).
1244:Siegel MS, Isacoff EY (1997).
732:
684:Modified Ace rhodopsin (Ace2)
457:Mac rhodopsin (FRET acceptor)
1:
3075:Yang HH, St-Pierre F (2016).
1569:10.1016/S0006-3495(02)75415-5
1265:10.1016/S0896-6273(00)80955-1
563:Voltage-gated proton channel
98:voltage sensitive phosphatase
2877:Lee EE, Bezanilla F (2017).
2296:10.1113/jphysiol.2013.257048
2150:10.1371/journal.pone.0043454
2039:10.1016/j.neuron.2012.06.040
1735:10.1371/journal.pone.0002514
1629:10.1371/journal.pone.0000440
1364:10.1021/acschemneuro.6b00234
867:10.1016/j.neuron.2012.06.040
758:Applications and advantages
667:Modified Archaerhodopsin 3
491:Modified Archaerhodopsin 3
474:Modified Archaerhodopsin 3
441:pair: CFP (seCFP2) and YFP
38:to a form of output, often
3551:
3326:10.1016/j.stem.2019.02.015
3234:10.1016/j.cbpa.2015.06.006
3188:10.1016/j.cell.2013.07.027
3146:10.1016/j.cell.2016.06.022
3036:10.1016/j.cell.2022.07.013
2999:: CS1 maint: postscript (
2795:10.1038/s41598-017-08731-2
2687:10.1016/j.cell.2016.05.031
2609:Kang BE, Baker BJ (2016).
2245:10.1016/j.bpj.2012.11.3773
1414:10.1016/j.cbpa.2015.05.006
974:10.1016/j.cell.2019.11.004
3487:
2963:10.1038/s41586-019-1166-7
2904:10.1016/j.bpj.2017.10.018
1306:10.1038/s41589-018-0004-9
1090:10.1038/s41586-019-1641-1
1024:10.1038/s41589-018-0004-9
381:Circularly permuted EGFP
61:green fluorescent protein
721:Circularly permuted GFP
704:Circularly permuted GFP
653:Circularly permuted GFP
636:Circularly permuted GFP
583:Circularly permuted GFP
566:Super ecliptic pHluorin
511:Circularly permuted GFP
3222:Curr. Opin. Chem. Biol.
2525:10.1126/science.aab0810
1939:10.1126/science.1204763
1402:Curr. Opin. Chem. Biol.
1294:Nature Chemical Biology
1156:10.1126/science.aab0810
1012:Nature Chemical Biology
530:FRET doner: mNeonGreen
527:Modified Ace rhodopsin
772:voltage sensitive dyes
729:
154:Rat Kv2.1 (K channel)
3479:Voltage-sensitive dye
3400:Optogenetic actuators
3275:10.3390/ijms160921626
3030:(18): 3408–3425.e29.
2197:10.1152/jn.00452.2012
805:optogenetic actuators
1975:J. Neurosci. Methods
1664:J. Neurosci. Methods
968:(7): 1590–1608.e23.
828:Openoptogenetics.org
48:electrophysiological
42:. It is a promising
3530:Protein engineering
3441:Optogenetic sensors
2955:2019Natur.569..413A
2896:2017BpJ...113.2178L
2845:10.7554/eLife.25690
2787:2017NatSR...7.8286L
2628:2016NatSR...623865K
2577:10.7554/eLife.10482
2517:2015Sci...350.1361G
2511:(6266): 1361–1366.
2455:2014NatCo...5.4894F
2346:2014NatCo...5.3674G
2283:J. Physiol. (Lond.)
2237:2013BpJ...104..683L
2141:2012PLoSO...743454B
1931:2011Sci...333..345K
1726:2008PLoSO...3.2514L
1620:2007PLoSO...2..440D
1561:2002BpJ....82..509A
1555:(1 Pt 1): 509–516.
1469:10.1038/nature03650
1461:2005Natur.435.1239M
1455:(7046): 1239–1243.
1352:ACS Chem. Neurosci.
1204:1968Natur.218..438C
1147:2015Sci...350.1361G
1082:2019Natur.574..413P
137:Shaker (K channel)
110:
3472:Related techniques
3268:(9): 21626–21642.
2463:10.1038/ncomms5894
2405:10.1038/nmeth.3000
2354:10.1038/ncomms4674
2090:10.1038/nmeth.1782
1783:10.1038/nmeth.1235
279:Red-shifted VSFP's
211:Kv1.4 (K channel)
160:pair: CFP and YFP
108:
94:Ciona intestinalis
76:directed evolution
32:membrane potential
3535:Electrophysiology
3507:
3506:
3427:Bacteriorhodopsin
3319:(5): 802–811.e5.
3262:Int. J. Mol. Sci.
3088:(39): 9977–9989.
2949:(7756): 413–417.
2890:(10): 2178–2181.
2636:10.1038/srep23865
2290:(18): 4427–4437.
1925:(6040): 345–348.
1875:(12): 1268–1277.
1825:(21): 5582–5593.
1507:(12): 2314–2318.
1501:Eur. J. Neurosci.
1198:(5140): 438–441.
1076:(7778): 413–417.
798:Future directions
728:
727:
468:QuasAr1, QuasAr2
360:Archaerhodopsin 3
40:fluorescent level
3542:
3495:
3494:
3411:Anion-conducting
3407:Channelrhodopsin
3386:
3379:
3372:
3363:
3357:
3356:
3346:
3328:
3304:
3298:
3297:
3287:
3277:
3252:
3246:
3245:
3216:
3210:
3209:
3199:
3165:
3159:
3158:
3148:
3122:
3116:
3115:
3105:
3072:
3066:
3065:
3055:
3014:
3005:
3004:
2998:
2990:
2974:
2932:
2926:
2925:
2915:
2874:
2868:
2867:
2857:
2847:
2823:
2817:
2816:
2806:
2765:
2759:
2758:
2748:
2731:(8): 2458–2472.
2715:
2709:
2708:
2698:
2664:
2658:
2657:
2647:
2606:
2600:
2599:
2589:
2579:
2553:
2547:
2546:
2536:
2494:
2485:
2484:
2474:
2433:
2427:
2426:
2416:
2382:
2376:
2375:
2365:
2324:
2318:
2317:
2307:
2273:
2267:
2266:
2256:
2231:(2): 1005–1012.
2215:
2209:
2208:
2191:(8): 2323–2337.
2185:J. Neurophysiol.
2179:
2173:
2172:
2162:
2152:
2118:
2112:
2111:
2101:
2067:
2061:
2060:
2050:
2016:
2010:
2009:
1999:
1965:
1959:
1958:
1912:
1903:
1902:
1892:
1859:
1853:
1852:
1842:
1809:
1803:
1802:
1764:
1758:
1757:
1747:
1737:
1703:
1697:
1696:
1658:
1652:
1651:
1641:
1631:
1597:
1591:
1590:
1580:
1539:
1533:
1532:
1495:
1489:
1488:
1442:
1436:
1435:
1425:
1392:
1386:
1385:
1375:
1342:
1336:
1335:
1325:
1284:
1278:
1277:
1267:
1241:
1232:
1231:
1212:10.1038/218438a0
1185:
1179:
1178:
1168:
1158:
1141:(6266): 1361–6.
1126:
1120:
1119:
1109:
1060:
1054:
1053:
1043:
1002:
996:
995:
985:
952:
943:
942:
932:
899:
890:
889:
879:
869:
845:
839:
838:
836:
834:
820:
737:
718:Modified Gg-VSD
701:Modified Gg-VSD
661:(pa)QuasAr3(-s)
650:Modified Gg-VSD
633:Modified Gg-VSD
580:Modified Gg-VSD
508:Modified Gg-VSD
111:
3550:
3549:
3545:
3544:
3543:
3541:
3540:
3539:
3510:
3509:
3508:
3503:
3483:
3467:
3436:
3432:Proteorhodopsin
3422:Archaerhodopsin
3395:
3390:
3360:
3306:
3305:
3301:
3254:
3253:
3249:
3218:
3217:
3213:
3167:
3166:
3162:
3124:
3123:
3119:
3074:
3073:
3069:
3016:
3015:
3008:
2991:
2988:
2986:
2984:
2934:
2933:
2929:
2876:
2875:
2871:
2825:
2824:
2820:
2767:
2766:
2762:
2717:
2716:
2712:
2666:
2665:
2661:
2608:
2607:
2603:
2555:
2554:
2550:
2496:
2495:
2488:
2435:
2434:
2430:
2384:
2383:
2379:
2326:
2325:
2321:
2275:
2274:
2270:
2217:
2216:
2212:
2181:
2180:
2176:
2120:
2119:
2115:
2069:
2068:
2064:
2018:
2017:
2013:
1967:
1966:
1962:
1914:
1913:
1906:
1861:
1860:
1856:
1811:
1810:
1806:
1766:
1765:
1761:
1705:
1704:
1700:
1660:
1659:
1655:
1599:
1598:
1594:
1541:
1540:
1536:
1497:
1496:
1492:
1444:
1443:
1439:
1395:Gong Y (2015).
1394:
1393:
1389:
1344:
1343:
1339:
1286:
1285:
1281:
1243:
1242:
1235:
1187:
1186:
1182:
1128:
1127:
1123:
1062:
1061:
1057:
1004:
1003:
999:
954:
953:
946:
921:10.1038/nn.3709
901:
900:
893:
847:
846:
842:
832:
830:
822:
821:
817:
813:
800:
768:calcium imaging
764:electrode-based
760:
744:
742:Characteristics
731:
389:VSFP-Butterfly
320:Nv-VSD, Dr-VSD
314:Zahra, Zahra 2
174:Rat Na channel
84:
72:rational design
56:
30:that can sense
17:
12:
11:
5:
3548:
3546:
3538:
3537:
3532:
3527:
3522:
3512:
3511:
3505:
3504:
3502:
3501:
3488:
3485:
3484:
3482:
3481:
3475:
3473:
3469:
3468:
3466:
3465:
3460:
3455:
3450:
3444:
3442:
3438:
3437:
3435:
3434:
3429:
3424:
3419:
3414:
3403:
3401:
3397:
3396:
3391:
3389:
3388:
3381:
3374:
3366:
3359:
3358:
3313:Cell Stem Cell
3299:
3247:
3211:
3182:(4): 904–913.
3160:
3117:
3067:
3006:
2927:
2869:
2818:
2760:
2710:
2681:(1): 245–257.
2659:
2601:
2548:
2486:
2428:
2399:(8): 825–833.
2377:
2319:
2268:
2210:
2174:
2113:
2062:
2033:(5): 779–785.
2011:
1982:(2): 190–196.
1960:
1904:
1854:
1804:
1777:(8): 683–685.
1759:
1698:
1653:
1592:
1534:
1490:
1437:
1387:
1358:(3): 513–523.
1337:
1300:(4): 352–360.
1279:
1258:(4): 735–741.
1233:
1180:
1121:
1055:
1018:(4): 352–360.
997:
944:
915:(6): 884–889.
909:Nat. Neurosci.
891:
840:
814:
812:
809:
799:
796:
759:
756:
748:photostability
743:
740:
739:
738:
726:
725:
722:
719:
716:
713:
709:
708:
705:
702:
699:
696:
692:
691:
688:
685:
682:
679:
675:
674:
671:
668:
665:
662:
658:
657:
654:
651:
648:
645:
641:
640:
637:
634:
631:
628:
624:
623:
618:
615:
612:
609:
605:
604:
601:
598:
595:
592:
588:
587:
584:
581:
578:
575:
571:
570:
567:
564:
561:
558:
554:
553:
550:
547:
544:
541:
537:
536:
531:
528:
525:
522:
516:
515:
512:
509:
506:
503:
499:
498:
495:
492:
489:
486:
482:
481:
478:
475:
472:
469:
465:
464:
461:
458:
455:
452:
446:
445:
442:
436:
433:
430:
426:
425:
422:
416:
413:
410:
406:
405:
402:
396:
393:
390:
386:
385:
382:
379:
376:
373:
369:
368:
365:
362:
357:
354:
350:
349:
346:
343:
340:
337:
331:
330:
327:
321:
318:
315:
311:
310:
307:
304:
301:
298:
294:
293:
290:
287:
284:
281:
275:
274:
271:
268:
263:
260:
256:
255:
252:
246:
243:
240:
236:
235:
232:
229:
226:
223:
219:
218:
215:
212:
209:
206:
202:
201:
198:
192:
189:
186:
182:
181:
178:
175:
172:
169:
165:
164:
161:
155:
152:
149:
145:
144:
141:
138:
135:
132:
128:
127:
124:
121:
118:
115:
83:
80:
55:
52:
15:
13:
10:
9:
6:
4:
3:
2:
3547:
3536:
3533:
3531:
3528:
3526:
3525:Biotechnology
3523:
3521:
3518:
3517:
3515:
3500:
3499:
3490:
3489:
3486:
3480:
3477:
3476:
3474:
3470:
3464:
3461:
3459:
3456:
3454:
3451:
3449:
3446:
3445:
3443:
3439:
3433:
3430:
3428:
3425:
3423:
3420:
3418:
3417:Halorhodopsin
3415:
3412:
3408:
3405:
3404:
3402:
3398:
3394:
3387:
3382:
3380:
3375:
3373:
3368:
3367:
3364:
3354:
3350:
3345:
3340:
3336:
3332:
3327:
3322:
3318:
3314:
3310:
3303:
3300:
3295:
3291:
3286:
3281:
3276:
3271:
3267:
3264:
3263:
3258:
3251:
3248:
3243:
3239:
3235:
3231:
3227:
3224:
3223:
3215:
3212:
3207:
3203:
3198:
3193:
3189:
3185:
3181:
3177:
3176:
3171:
3164:
3161:
3156:
3152:
3147:
3142:
3138:
3134:
3133:
3128:
3121:
3118:
3113:
3109:
3104:
3099:
3095:
3091:
3087:
3084:
3083:
3078:
3071:
3068:
3063:
3059:
3054:
3049:
3045:
3041:
3037:
3033:
3029:
3025:
3021:
3013:
3011:
3007:
3002:
2996:
2982:
2978:
2973:
2968:
2964:
2960:
2956:
2952:
2948:
2944:
2943:
2938:
2931:
2928:
2923:
2919:
2914:
2909:
2905:
2901:
2897:
2893:
2889:
2886:
2885:
2880:
2873:
2870:
2865:
2861:
2856:
2851:
2846:
2841:
2837:
2833:
2829:
2822:
2819:
2814:
2810:
2805:
2800:
2796:
2792:
2788:
2784:
2780:
2777:
2776:
2771:
2764:
2761:
2756:
2752:
2747:
2742:
2738:
2734:
2730:
2727:
2726:
2721:
2714:
2711:
2706:
2702:
2697:
2692:
2688:
2684:
2680:
2676:
2675:
2670:
2663:
2660:
2655:
2651:
2646:
2641:
2637:
2633:
2629:
2625:
2621:
2618:
2617:
2612:
2605:
2602:
2597:
2593:
2588:
2583:
2578:
2573:
2569:
2565:
2564:
2559:
2552:
2549:
2544:
2540:
2535:
2530:
2526:
2522:
2518:
2514:
2510:
2506:
2505:
2500:
2493:
2491:
2487:
2482:
2478:
2473:
2468:
2464:
2460:
2456:
2452:
2448:
2445:
2444:
2439:
2432:
2429:
2424:
2420:
2415:
2410:
2406:
2402:
2398:
2394:
2393:
2388:
2381:
2378:
2373:
2369:
2364:
2359:
2355:
2351:
2347:
2343:
2339:
2336:
2335:
2330:
2323:
2320:
2315:
2311:
2306:
2301:
2297:
2293:
2289:
2285:
2284:
2279:
2272:
2269:
2264:
2260:
2255:
2250:
2246:
2242:
2238:
2234:
2230:
2227:
2226:
2221:
2214:
2211:
2206:
2202:
2198:
2194:
2190:
2187:
2186:
2178:
2175:
2170:
2166:
2161:
2156:
2151:
2146:
2142:
2138:
2135:(9): e43454.
2134:
2130:
2129:
2124:
2117:
2114:
2109:
2105:
2100:
2095:
2091:
2087:
2083:
2079:
2078:
2073:
2066:
2063:
2058:
2054:
2049:
2044:
2040:
2036:
2032:
2028:
2027:
2022:
2015:
2012:
2007:
2003:
1998:
1993:
1989:
1985:
1981:
1977:
1976:
1971:
1964:
1961:
1956:
1952:
1948:
1944:
1940:
1936:
1932:
1928:
1924:
1920:
1919:
1911:
1909:
1905:
1900:
1896:
1891:
1886:
1882:
1878:
1874:
1871:
1870:
1865:
1858:
1855:
1850:
1846:
1841:
1836:
1832:
1828:
1824:
1821:
1820:
1815:
1808:
1805:
1800:
1796:
1792:
1788:
1784:
1780:
1776:
1772:
1771:
1763:
1760:
1755:
1751:
1746:
1741:
1736:
1731:
1727:
1723:
1719:
1715:
1714:
1709:
1702:
1699:
1694:
1690:
1686:
1682:
1678:
1674:
1670:
1666:
1665:
1657:
1654:
1649:
1645:
1640:
1635:
1630:
1625:
1621:
1617:
1613:
1609:
1608:
1603:
1596:
1593:
1588:
1584:
1579:
1574:
1570:
1566:
1562:
1558:
1554:
1551:
1550:
1545:
1538:
1535:
1530:
1526:
1522:
1518:
1514:
1510:
1506:
1503:
1502:
1494:
1491:
1486:
1482:
1478:
1474:
1470:
1466:
1462:
1458:
1454:
1450:
1449:
1441:
1438:
1433:
1429:
1424:
1419:
1415:
1411:
1407:
1404:
1403:
1398:
1391:
1388:
1383:
1379:
1374:
1369:
1365:
1361:
1357:
1354:
1353:
1348:
1341:
1338:
1333:
1329:
1324:
1319:
1315:
1311:
1307:
1303:
1299:
1295:
1291:
1283:
1280:
1275:
1271:
1266:
1261:
1257:
1253:
1252:
1247:
1240:
1238:
1234:
1229:
1225:
1221:
1217:
1213:
1209:
1205:
1201:
1197:
1193:
1192:
1184:
1181:
1176:
1172:
1167:
1162:
1157:
1152:
1148:
1144:
1140:
1136:
1132:
1125:
1122:
1117:
1113:
1108:
1103:
1099:
1095:
1091:
1087:
1083:
1079:
1075:
1071:
1067:
1059:
1056:
1051:
1047:
1042:
1037:
1033:
1029:
1025:
1021:
1017:
1013:
1009:
1001:
998:
993:
989:
984:
979:
975:
971:
967:
963:
959:
951:
949:
945:
940:
936:
931:
926:
922:
918:
914:
911:
910:
905:
898:
896:
892:
887:
883:
878:
873:
868:
863:
860:(5): 779–85.
859:
855:
851:
844:
841:
829:
825:
819:
816:
810:
808:
806:
797:
795:
793:
792:cardiomyocyte
789:
788:
783:
779:
778:
773:
769:
765:
757:
755:
753:
752:phototoxicity
749:
741:
735:
734:
730:
723:
720:
717:
714:
711:
710:
706:
703:
700:
697:
694:
693:
689:
686:
683:
680:
678:Voltron(-ST)
677:
676:
672:
670:Same as left
669:
666:
663:
660:
659:
655:
652:
649:
646:
643:
642:
638:
635:
632:
629:
626:
625:
622:
619:
616:
613:
610:
607:
606:
602:
599:
596:
593:
590:
589:
585:
582:
579:
576:
573:
572:
568:
565:
562:
559:
556:
555:
551:
548:
545:
542:
540:ArcLightning
539:
538:
535:
532:
529:
526:
523:
521:
518:
517:
513:
510:
507:
504:
501:
500:
496:
494:Same as left
493:
490:
487:
484:
483:
479:
477:Same as left
476:
473:
470:
467:
466:
462:
459:
456:
453:
451:
448:
447:
443:
440:
437:
434:
431:
428:
427:
423:
420:
417:
414:
411:
408:
407:
403:
400:
397:
394:
391:
388:
387:
383:
380:
377:
374:
371:
370:
366:
364:Same as left
363:
361:
358:
355:
352:
351:
347:
344:
341:
338:
336:
333:
332:
328:
325:
322:
319:
316:
313:
312:
308:
306:Same as left
305:
302:
299:
296:
295:
291:
288:
285:
282:
280:
277:
276:
272:
269:
267:
266:Dipicrylamine
264:
261:
258:
257:
253:
250:
247:
244:
241:
238:
237:
233:
230:
227:
224:
221:
220:
216:
213:
210:
207:
204:
203:
199:
196:
193:
190:
187:
184:
183:
179:
176:
173:
170:
167:
166:
162:
159:
156:
153:
150:
147:
146:
142:
139:
136:
133:
130:
129:
125:
122:
119:
116:
113:
112:
106:
103:
99:
96:
95:
88:
81:
79:
77:
73:
68:
66:
62:
53:
51:
49:
45:
41:
37:
33:
29:
25:
21:
3520:Neuroscience
3497:
3452:
3393:Optogenetics
3316:
3312:
3302:
3265:
3260:
3250:
3225:
3220:
3214:
3179:
3173:
3163:
3139:(1): 18–20.
3136:
3130:
3120:
3085:
3082:J. Neurosci.
3080:
3070:
3027:
3023:
2995:cite journal
2946:
2940:
2930:
2887:
2882:
2872:
2835:
2831:
2821:
2778:
2773:
2763:
2728:
2725:J. Neurosci.
2723:
2713:
2678:
2672:
2662:
2619:
2614:
2604:
2567:
2561:
2551:
2508:
2502:
2446:
2443:Nat. Commun.
2441:
2431:
2396:
2392:Nat. Methods
2390:
2380:
2337:
2334:Nat. Commun.
2332:
2322:
2287:
2281:
2271:
2228:
2223:
2213:
2188:
2183:
2177:
2132:
2126:
2116:
2084:(1): 90–95.
2081:
2077:Nat. Methods
2075:
2065:
2030:
2024:
2014:
1979:
1973:
1963:
1922:
1916:
1872:
1867:
1857:
1822:
1819:J. Neurosci.
1817:
1807:
1774:
1770:Nat. Methods
1768:
1762:
1720:(6): e2514.
1717:
1711:
1701:
1671:(1): 32–38.
1668:
1662:
1656:
1611:
1605:
1595:
1552:
1547:
1537:
1504:
1499:
1493:
1452:
1446:
1440:
1405:
1400:
1390:
1355:
1350:
1340:
1297:
1293:
1282:
1255:
1249:
1195:
1189:
1183:
1138:
1134:
1124:
1073:
1069:
1058:
1015:
1011:
1000:
965:
961:
912:
907:
857:
853:
843:
831:. Retrieved
827:
818:
801:
785:
775:
761:
745:
733:
533:
519:
449:
278:
92:
89:
85:
69:
57:
23:
19:
18:
2884:Biophys. J.
2781:(1): 8286.
2225:Biophys. J.
1869:Chem. Biol.
1614:(5): e440.
1549:Biophys. J.
372:ElectricPk
44:optogenetic
3514:Categories
2570:: e10482.
811:References
777:Drosophila
608:Bongwoori
126:Precursor
123:Reporting
3458:Glutamate
3335:1934-5909
3228:: 75–83.
3044:0092-8674
2775:Sci. Rep.
2622:: 23865.
2616:Sci. Rep.
1408:: 84–89.
1314:1552-4469
1098:1476-4687
1032:1552-4469
552:ArcLight
534:Mac GEVIs
520:Ace GEVIs
450:Mac GEVIs
429:Mermaid2
82:Structure
3353:30880024
3294:26370981
3242:26115448
3206:23932121
3155:27368098
3112:27683896
3062:35985322
2981:31043747
2922:29108650
2864:28749338
2813:28811673
2755:26911693
2705:27264607
2654:27040905
2596:26599732
2543:26586188
2481:25222271
2449:: 4894.
2423:24952910
2372:24755708
2340:: 3674.
2314:23836686
2263:22961245
2205:22815406
2169:22970127
2128:PLoS One
2108:22120467
2057:22958819
2006:22634212
1947:21764748
1899:20064437
1849:18495892
1799:30661869
1791:18622396
1754:18575613
1713:PLoS One
1685:17126911
1648:17487283
1607:PLoS One
1587:11751337
1529:10969720
1521:11454036
1477:15902207
1432:26143170
1382:28045247
1332:29483642
1175:26586188
1116:31597963
1050:29483642
992:31835034
939:24755780
886:22958819
782:bacteria
712:JEDI-2P
673:QuasAr2
621:ArcLight
603:VSFP3.1
444:Mermaid
424:VSFP2.3
409:VSFP-CR
404:VSFP2's
384:VSFP3.1
335:ArcLight
329:VSFP2's
292:VSFP3.1
254:VSFP2's
239:Mermaid
234:VSFP2's
222:VSFP3.1
185:VSFP2's
120:Sensing
3498:Commons
3463:Vesicle
3453:Voltage
3448:Calcium
3344:6499654
3285:4613271
3197:3874294
3103:5039263
3053:9563101
2972:6613938
2951:Bibcode
2913:5700382
2892:Bibcode
2855:5584994
2804:5557843
2783:Bibcode
2746:4764664
2696:5606228
2645:4878010
2624:Bibcode
2587:4658195
2534:4904846
2513:Bibcode
2504:Science
2472:4166526
2451:Bibcode
2414:4117813
2363:4247277
2342:Bibcode
2305:3784191
2254:3461113
2233:Bibcode
2160:3435330
2137:Bibcode
2099:3248630
2048:3439164
1997:3398169
1955:2195943
1927:Bibcode
1918:Science
1890:2818747
1840:2714581
1745:2429971
1722:Bibcode
1693:8540453
1639:1857823
1616:Bibcode
1578:1302490
1557:Bibcode
1485:4427755
1457:Bibcode
1423:4571180
1373:5355904
1323:5866759
1274:9354320
1228:4288546
1220:5649693
1200:Bibcode
1166:4904846
1143:Bibcode
1135:Science
1107:6858559
1078:Bibcode
1064:2019).
1041:5866759
983:6941988
930:4494739
877:3439164
787:E. coli
724:ASAP2s
707:ASAP2s
644:ASAP-Y
627:ASAP2s
614:Ci-VSD
597:Ci-VSD
591:FlicR1
574:ASAP2f
546:Ci-VSD
485:Archer
435:Ci-VSD
415:Ci-VSD
395:Ci-VSD
378:Ci-VSD
342:Ci-VSD
286:Ci-VSD
245:Ci-VSD
228:Ci-VSD
191:Ci-VSD
102:genomic
54:History
36:voltage
28:protein
26:) is a
16:Protein
3351:
3341:
3333:
3292:
3282:
3240:
3204:
3194:
3153:
3110:
3100:
3060:
3050:
3042:
2979:
2969:
2942:Nature
2920:
2910:
2862:
2852:
2811:
2801:
2753:
2743:
2703:
2693:
2652:
2642:
2594:
2584:
2541:
2531:
2479:
2469:
2421:
2411:
2370:
2360:
2312:
2302:
2261:
2251:
2203:
2167:
2157:
2106:
2096:
2055:
2045:
2026:Neuron
2004:
1994:
1953:
1945:
1897:
1887:
1847:
1837:
1797:
1789:
1752:
1742:
1691:
1683:
1646:
1636:
1585:
1575:
1527:
1519:
1483:
1475:
1448:Nature
1430:
1420:
1380:
1370:
1330:
1320:
1312:
1272:
1251:Neuron
1226:
1218:
1191:Nature
1173:
1163:
1114:
1104:
1096:
1070:Nature
1048:
1038:
1030:
990:
980:
937:
927:
884:
874:
854:Neuron
695:ASAP3
656:ASAP1
639:ASAP1
586:ASAP1
502:ASAP1
297:PROPS
217:FlaSh
205:Flare
200:VSFP1
168:SPARC
148:VSFP1
131:FlaSh
65:Shaker
2832:eLife
2563:eLife
1951:S2CID
1795:S2CID
1689:S2CID
1525:S2CID
1481:S2CID
1224:S2CID
833:8 May
770:, or
715:2022
698:2019
681:2019
664:2019
647:2017
630:2017
611:2017
594:2016
577:2016
560:2016
557:Pado
543:2015
524:2015
505:2014
497:Arch
488:2014
480:Arch
471:2014
454:2014
432:2013
412:2013
392:2012
375:2012
356:2012
353:Arch
339:2012
317:2012
300:2011
283:2009
262:2008
259:hVOS
242:2008
225:2008
208:2007
188:2007
171:2002
151:2001
134:1997
117:Year
114:GEVI
3349:PMID
3331:ISSN
3290:PMID
3238:PMID
3202:PMID
3175:Cell
3151:PMID
3132:Cell
3108:PMID
3058:PMID
3040:ISSN
3024:Cell
3001:link
2977:PMID
2918:PMID
2860:PMID
2809:PMID
2751:PMID
2701:PMID
2674:Cell
2650:PMID
2592:PMID
2539:PMID
2477:PMID
2419:PMID
2368:PMID
2310:PMID
2259:PMID
2201:PMID
2165:PMID
2104:PMID
2053:PMID
2002:PMID
1943:PMID
1895:PMID
1845:PMID
1787:PMID
1750:PMID
1681:PMID
1644:PMID
1583:PMID
1517:PMID
1473:PMID
1428:PMID
1378:PMID
1328:PMID
1310:ISSN
1270:PMID
1216:PMID
1171:PMID
1112:PMID
1094:ISSN
1046:PMID
1028:ISSN
988:PMID
962:Cell
935:PMID
882:PMID
835:2017
439:FRET
419:FRET
399:FRET
324:FRET
270:GFP
249:FRET
231:CFP
214:YFP
195:FRET
177:GFP
158:FRET
140:GFP
74:and
24:GEVI
22:(or
3339:PMC
3321:doi
3280:PMC
3270:doi
3230:doi
3192:PMC
3184:doi
3180:154
3141:doi
3137:166
3098:PMC
3090:doi
3048:PMC
3032:doi
3028:185
2967:PMC
2959:doi
2947:569
2908:PMC
2900:doi
2888:113
2850:PMC
2840:doi
2799:PMC
2791:doi
2741:PMC
2733:doi
2691:PMC
2683:doi
2679:166
2640:PMC
2632:doi
2582:PMC
2572:doi
2529:PMC
2521:doi
2509:350
2467:PMC
2459:doi
2409:PMC
2401:doi
2358:PMC
2350:doi
2300:PMC
2292:doi
2288:591
2249:PMC
2241:doi
2229:104
2193:doi
2189:108
2155:PMC
2145:doi
2094:PMC
2086:doi
2043:PMC
2035:doi
1992:PMC
1984:doi
1980:208
1935:doi
1923:333
1885:PMC
1877:doi
1835:PMC
1827:doi
1779:doi
1740:PMC
1730:doi
1673:doi
1669:161
1634:PMC
1624:doi
1573:PMC
1565:doi
1509:doi
1465:doi
1453:435
1418:PMC
1410:doi
1368:PMC
1360:doi
1318:PMC
1302:doi
1260:doi
1208:doi
1196:218
1161:PMC
1151:doi
1139:350
1102:PMC
1086:doi
1074:574
1036:PMC
1020:doi
978:PMC
970:doi
966:179
925:PMC
917:doi
872:PMC
862:doi
3516::
3347:.
3337:.
3329:.
3317:24
3315:.
3311:.
3288:.
3278:.
3266:16
3259:.
3236:.
3226:27
3200:.
3190:.
3178:.
3172:.
3149:.
3135:.
3129:.
3106:.
3096:.
3086:36
3079:.
3056:.
3046:.
3038:.
3026:.
3022:.
3009:^
2997:}}
2993:{{
2975:.
2965:.
2957:.
2945:.
2939:.
2916:.
2906:.
2898:.
2881:.
2858:.
2848:.
2838:.
2834:.
2830:.
2807:.
2797:.
2789:.
2772:.
2749:.
2739:.
2729:36
2722:.
2699:.
2689:.
2677:.
2671:.
2648:.
2638:.
2630:.
2613:.
2590:.
2580:.
2566:.
2560:.
2537:.
2527:.
2519:.
2507:.
2501:.
2489:^
2475:.
2465:.
2457:.
2440:.
2417:.
2407:.
2397:11
2395:.
2389:.
2366:.
2356:.
2348:.
2331:.
2308:.
2298:.
2286:.
2280:.
2257:.
2247:.
2239:.
2222:.
2199:.
2163:.
2153:.
2143:.
2131:.
2125:.
2102:.
2092:.
2080:.
2074:.
2051:.
2041:.
2031:75
2029:.
2023:.
2000:.
1990:.
1978:.
1972:.
1949:.
1941:.
1933:.
1921:.
1907:^
1893:.
1883:.
1873:16
1866:.
1843:.
1833:.
1823:28
1816:.
1793:.
1785:.
1773:.
1748:.
1738:.
1728:.
1716:.
1710:.
1687:.
1679:.
1667:.
1642:.
1632:.
1622:.
1610:.
1604:.
1581:.
1571:.
1563:.
1553:82
1546:.
1523:.
1515:.
1505:13
1479:.
1471:.
1463:.
1451:.
1426:.
1416:.
1406:27
1399:.
1376:.
1366:.
1349:.
1326:.
1316:.
1308:.
1298:14
1296:.
1292:.
1268:.
1256:19
1254:.
1248:.
1236:^
1222:.
1214:.
1206:.
1194:.
1169:.
1159:.
1149:.
1137:.
1133:.
1110:.
1100:.
1092:.
1084:.
1072:.
1068:.
1044:.
1034:.
1026:.
1016:14
1014:.
1010:.
986:.
976:.
964:.
960:.
947:^
933:.
923:.
913:17
906:.
894:^
880:.
870:.
858:75
856:.
852:.
826:.
794:.
690:-
569:-
514:-
463:-
367:-
348:-
309:-
273:-
180:-
163:-
143:-
3413:)
3409:(
3385:e
3378:t
3371:v
3355:.
3323::
3296:.
3272::
3244:.
3232::
3208:.
3186::
3157:.
3143::
3114:.
3092::
3064:.
3034::
3003:)
2983:.
2961::
2953::
2924:.
2902::
2894::
2866:.
2842::
2836:6
2815:.
2793::
2785::
2779:7
2757:.
2735::
2707:.
2685::
2656:.
2634::
2626::
2620:6
2598:.
2574::
2568:4
2545:.
2523::
2515::
2483:.
2461::
2453::
2447:5
2425:.
2403::
2374:.
2352::
2344::
2338:5
2316:.
2294::
2265:.
2243::
2235::
2207:.
2195::
2171:.
2147::
2139::
2133:7
2110:.
2088::
2082:9
2059:.
2037::
2008:.
1986::
1957:.
1937::
1929::
1901:.
1879::
1851:.
1829::
1801:.
1781::
1775:5
1756:.
1732::
1724::
1718:3
1695:.
1675::
1650:.
1626::
1618::
1612:2
1589:.
1567::
1559::
1531:.
1511::
1487:.
1467::
1459::
1434:.
1412::
1384:.
1362::
1356:8
1334:.
1304::
1276:.
1262::
1230:.
1210::
1202::
1177:.
1153::
1145::
1118:.
1088::
1080::
1052:.
1022::
994:.
972::
941:.
919::
888:.
864::
837:.
784:(
Text is available under the Creative Commons Attribution-ShareAlike License. Additional terms may apply.