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pair. Sticky ends of DNA however are more likely to successfully bind with the help of a DNA ligase because of the exposed and unpaired nucleotides. For example, a sticky end trailing with AATTG is more likely to bind with a ligase than a blunt end where both the 5' and 3' DNA strands are paired. In the case of the example the AATTG would have a complementary pair of TTAAC which would reduce the functionality of the DNA ligase enzyme.
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Some restriction enzymes cut DNA at a restriction site in a manner which leaves no overhang, called a blunt end. Blunt ends are much less likely to be ligated by a DNA ligase because the blunt end doesn't have the overhanging base pair that the enzyme can recognize and match with a complementary
109:(i.e. short absent motifs which are highly expected to exist) in virus genomes are restriction sites indicating that viruses have probably got rid of these motifs to facilitate invasion of bacterial hosts.
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Gao, Song; Zhang, Jiannan; Miao, Tianjin; Ma, Di; Su, Ying; An, Yingfeng; Zhang, Qingrui (28 March 2015). "A Simple and
Convenient Sticky/Blunt-End Ligation Method for Fusion Gene Construction".
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Several databases exist for restriction sites and enzymes, of which the largest noncommercial database is REBASE. Recently, it has been shown that statistically significant
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recognizes the palindromic sequence GAATTC and cuts between the G and the A on both the top and bottom strands. This leaves an overhang (an end-portion of a
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Restriction sites can be used for multiple applications in molecular biology such as identifying restriction fragment length polymorphisms (
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61:), and a particular restriction enzyme may cut the sequence between two nucleotides within its recognition site, or somewhere nearby.
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contains a comprehensive catalogue of minimal absent motifs many of which might potentially be not-yet-known restriction motifs.
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strand with no attached complement) known as a sticky end on each end of AATT. The overhang can then be used to ligate in (see
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81:) a piece of DNA with a complementary overhang (another EcoRI-cut piece, for example).
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344:"REBASE—a database for DNA restriction and modification: enzymes, genes and genomes"
287:"REBASE—a database for DNA restriction and modification: enzymes, genes and genomes"
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211:"Fabrication of DNA nanotubes using origami-based nanostructures with sticky ends"
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Roberts, Richard J.; Vincze, Tamas; Posfai, Janos; Macelis, Dana (2014-11-05).
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Roberts, Richard J.; Vincze, Tamas; Posfai, Janos; Macelis, Dana (2009-10-21).
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Mousavi-Khattat, Mohammad; Rafati, Adele; Gill, Pooria (5 February 2015).
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molecule containing specific (4-8 base pairs in length) sequences of
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401:"Significant non-existence of sequences in genomes and proteomes"
184:(5th ed.). New York, NY: W.H. Freeman and Company. p.
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Lehninger, Albert L.; Nelson, David L.; Cox, Michael M. (2008).
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Koulouras, Grigorios; Frith, Martin C (2021-04-06).
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57:(because restriction enzymes usually bind as
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69:For example, the common restriction enzyme
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123:List of restriction enzyme cutting sites
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215:Journal of Nanostructure in Chemistry
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146:iGenetics: A Mendelian Approach
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39:restriction recognition sites
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180:Principles of Biochemistry
142:Russell, Peter J. (2006).
49:, which are recognized by
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256:10.1007/s10528-015-9669-x
228:10.1007/s40097-015-0148-z
27:Not to be confused with
405:Nucleic Acids Research
348:Nucleic Acids Research
297:(suppl_1): D234–D236.
291:Nucleic Acids Research
53:. These are generally
150:. Benjamin Cummings.
55:palindromic sequences
244:Biochemical Genetics
29:Recognition sequence
475:Restriction enzymes
465:Genetics techniques
418:10.1093/nar/gkab139
360:10.1093/nar/gku1046
51:restriction enzymes
41:, are located on a
303:10.1093/nar/gkp874
111:Nullomers Database
470:Molecular biology
354:(D1): D298–D299.
195:978-0-7167-7108-1
35:Restriction sites
18:Restriction sites
16:(Redirected from
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47:nucleotides
459:Categories
129:References
79:DNA ligase
59:homodimers
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311:0305-1048
107:nullomers
101:Databases
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386:25378308
329:19846593
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117:See also
65:Function
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95:RFLPs
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37:, or
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382:PMID
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325:PMID
307:ISSN
260:PMID
190:ISBN
152:ISBN
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