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Immunophenotyping

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Immunophenotyping is a very common flow cytometry test in which fluorophore-conjugated antibodies are used as probes for staining target cells with high avidity and affinity. This technique allows rapid and easy phenotyping of each cell in a heterogeneous sample according to the presence or absence
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is a technique used to study the protein expressed by cells. This technique is commonly used in basic science research and laboratory diagnostic purpose. This can be done on tissue section (fresh or fixed tissue),
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Young, Yoon Kow; Bolt, Alicia M.; Ahn, Ryuhjin; Mann, Koren K. (2016). "Analyzing the Tumor Microenvironment by Flow Cytometry".
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on their membrane. By choosing appropriate antibodies, the differentiation of
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cells can be accurately determined. The labelled cells are processed in a
52: 44: 36: 48: 60: 8: 149:British Society for Haematology guidelines 157:at the U.S. National Library of Medicine 84: 27:, etc. An example is the detection of 16:Classifying cells of the immune system 7: 100:. Vol. 1458. pp. 95–110. 14: 39:. It involves the labelling of 1: 98:Methods in Molecular Biology 71:in a matter of a few hours. 106:10.1007/978-1-4939-3801-8_8 75:of a protein combination. 196: 94:The Tumor Microenvironment 47:directed against surface 159:Medical Subject Headings 151:accessed July 31, 2006 155:Immunophenotyping 115:978-1-4939-3799-8 41:white blood cells 31:, such as in the 20:Immunophenotyping 187: 136: 135: 89: 195: 194: 190: 189: 188: 186: 185: 184: 165: 164: 145: 140: 139: 116: 91: 90: 86: 81: 25:cell suspension 17: 12: 11: 5: 193: 191: 183: 182: 177: 175:Flow cytometry 167: 166: 163: 162: 152: 144: 143:External links 141: 138: 137: 114: 83: 82: 80: 77: 57:flow cytometer 15: 13: 10: 9: 6: 4: 3: 2: 192: 181: 178: 176: 173: 172: 170: 160: 156: 153: 150: 147: 146: 142: 133: 129: 125: 121: 117: 111: 107: 103: 99: 95: 88: 85: 78: 76: 72: 70: 66: 62: 58: 54: 50: 46: 42: 38: 34: 30: 29:tumor markers 26: 21: 93: 87: 73: 69:spinal fluid 19: 18: 65:bone marrow 180:Hematology 169:Categories 79:References 45:antibodies 124:1940-6029 33:diagnosis 132:27581017 53:leukemic 49:proteins 37:leukemia 161:(MeSH) 130:  122:  112:  61:blood 43:with 128:PMID 120:ISSN 110:ISBN 102:doi 67:or 35:of 171:: 126:. 118:. 108:. 96:. 63:, 134:. 104::

Index

cell suspension
tumor markers
diagnosis
leukemia
white blood cells
antibodies
proteins
leukemic
flow cytometer
blood
bone marrow
spinal fluid
Methods in Molecular Biology
doi
10.1007/978-1-4939-3801-8_8
ISBN
978-1-4939-3799-8
ISSN
1940-6029
PMID
27581017
British Society for Haematology guidelines
Immunophenotyping
Medical Subject Headings
Categories
Flow cytometry
Hematology

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