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Confluency

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29: 176:, and may continue to divide and form layers on top of the parent cells. To achieve optimal and consistent results, experiments are usually performed using cells at a particular confluence, depending on the cell type. Extracellular export of cell free material is also dependent on the cell confluence . 141:. For example, 50 percent confluence means roughly half of the surface is covered, while 100 percent confluence means the surface is completely covered by the cells, and no more room is left for the cells to grow as a 189:
Comparing the amount of space covered by cells with unoccupied space using the naked eye can provide a rough estimate of confluency.
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Many cell lines exhibit differences in growth rate or gene expression depending on the degree of confluence. Cells are typically
50: 93: 65: 46: 17: 250:"Polysome arrest restricts miRNA turnover by preventing exosomal export of miRNA in growth-retarded mammalian cells" 72: 39: 79: 224: 303: 61: 158: 173: 169: 279: 269: 261: 86: 274: 249: 138: 297: 204: 199: 134: 126: 225:"Cell Counting and Confluency Analysis as Quality Controls in Cell-Based Assays" 162: 28: 265: 165: 142: 283: 248:
Ghosh, S; Bose, M; Ray, A; Bhattacharyya, SN (15 March 2015).
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refers to, trivially, the number of cells in a given region.
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before becoming fully confluent in order to maintain their
53:. Unsourced material may be challenged and removed. 133:refers to the percentage of the surface of a 8: 273: 113:Learn how and when to remove this message 216: 168:. Some cell types are not limited by 7: 51:adding citations to reliable sources 14: 27: 38:needs additional citations for 1: 254:Molecular Biology of the Cell 137:that is covered by adherent 207:, giving the cell number. 18:Confluence (disambiguation) 320: 15: 266:10.1091/mbc.E14-11-1521 47:improve this article 16:For other uses, see 174:immortalized cells 170:contact inhibition 153:Impact on research 123: 122: 115: 97: 311: 288: 287: 277: 245: 239: 238: 236: 234: 229: 221: 118: 111: 107: 104: 98: 96: 55: 31: 23: 319: 318: 314: 313: 312: 310: 309: 308: 294: 293: 292: 291: 247: 246: 242: 232: 230: 227: 223: 222: 218: 213: 203:can be used to 195: 187: 182: 155: 119: 108: 102: 99: 56: 54: 44: 32: 21: 12: 11: 5: 317: 315: 307: 306: 296: 295: 290: 289: 260:(6): 1072–83. 240: 215: 214: 212: 209: 194: 191: 186: 183: 181: 178: 154: 151: 121: 120: 35: 33: 26: 13: 10: 9: 6: 4: 3: 2: 316: 305: 302: 301: 299: 285: 281: 276: 271: 267: 263: 259: 255: 251: 244: 241: 226: 220: 217: 210: 208: 206: 202: 201: 200:hemocytometer 193:Hemocytometer 192: 190: 185:Rule of thumb 184: 179: 177: 175: 171: 167: 164: 163:proliferation 160: 152: 150: 148: 144: 140: 136: 132: 128: 117: 114: 106: 95: 92: 88: 85: 81: 78: 74: 71: 67: 64: –  63: 59: 58:Find sources: 52: 48: 42: 41: 36:This article 34: 30: 25: 24: 19: 304:Cell culture 257: 253: 243: 231:. Retrieved 219: 198: 196: 188: 156: 146: 135:culture dish 130: 127:cell culture 124: 109: 100: 90: 83: 76: 69: 62:"Confluency" 57: 45:Please help 40:verification 37: 205:count cells 147:cell number 211:References 180:Estimation 172:, such as 131:confluence 73:newspapers 166:phenotype 143:monolayer 129:biology, 103:July 2018 298:Category 284:25609084 159:passaged 275:4357507 87:scholar 282:  272:  233:4 July 145:. The 89:  82:  75:  68:  60:  228:(PDF) 139:cells 94:JSTOR 80:books 280:PMID 235:2018 66:news 270:PMC 262:doi 125:In 49:by 300:: 278:. 268:. 258:26 256:. 252:. 197:A 286:. 264:: 237:. 116:) 110:( 105:) 101:( 91:· 84:· 77:· 70:· 43:. 20:.

Index

Confluence (disambiguation)

verification
improve this article
adding citations to reliable sources
"Confluency"
news
newspapers
books
scholar
JSTOR
Learn how and when to remove this message
cell culture
culture dish
cells
monolayer
passaged
proliferation
phenotype
contact inhibition
immortalized cells
hemocytometer
count cells
"Cell Counting and Confluency Analysis as Quality Controls in Cell-Based Assays"
"Polysome arrest restricts miRNA turnover by preventing exosomal export of miRNA in growth-retarded mammalian cells"
doi
10.1091/mbc.E14-11-1521
PMC
4357507
PMID

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