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176:, and may continue to divide and form layers on top of the parent cells. To achieve optimal and consistent results, experiments are usually performed using cells at a particular confluence, depending on the cell type. Extracellular export of cell free material is also dependent on the cell confluence .
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Comparing the amount of space covered by cells with unoccupied space using the naked eye can provide a rough estimate of confluency.
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Many cell lines exhibit differences in growth rate or gene expression depending on the degree of confluence. Cells are typically
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Ghosh, S; Bose, M; Ray, A; Bhattacharyya, SN (15 March 2015).
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before becoming fully confluent in order to maintain their
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