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proteins. It usually means that the deleterious mutation and the suppressive mutation occurs in two residues that are closely located in the tridimensional structure of the multi-protein complex. As thus, this kind of suppression provides indirect information on the molecular structure of the proteins involved.
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Dosage-mediated suppression occurs when the suppression of the mutant phenotype is mediated by the over expression of a second suppressor gene. This can occur when the initial mutations destabilize a protein-protein interaction and over expression of the interacting protein bypass the negative effect
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in the growing polypeptide chain can mutate so that it recognize a TAA stop codon and promote the insertion of serine instead of the termination of the polypeptide chain. This could be particularly useful when a nonsense mutation (TCA >TAA) prevents the expression of a gene by either leading to a
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The strongest form of synthetic rescues, in which the deleterious impact of a gene knockout is mitigated by an additional genetic perturbation that is also deleterious when considered in isolation, was modeled and predicted theoretically for gene interactions mediated by the metabolic network. This
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Interaction-mediated suppression occurs when a deleterious mutation in a component of a protein complex destabilizes the complex. A compensatory mutation in another component of the protein complex can then suppress the deleterious phenotype by re-establishing the interaction between the two
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Puddu, F.; Oelschlaegel, T; Guerini, I; Geisler, NJ; Niu, H; Herzog, M; Salguero, I; Ochoa-Montaño, B; Viré, E; Sung, P; Adams, DJ; Keane, TM; Jackson, SP (2015).
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is rescued ) refers to a genetic interaction in which a cell that is nonviable, sensitive to a specific drug, or otherwise impaired due to the presence of a
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becomes viable when the original mutation is combined with a second mutation in a different gene. The second mutation can either be a
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Experimental evolution of diverse
Escherichia coli metabolic mutants identifies genetic loci for convergent adaptation of growth rate
319:"Beyond Synthetic Lethality: Charting the Landscape of Pairwise Gene Expression States Associated with Survival in Cancer"
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sequence. For example, a tRNA designated for the recognition of the codon TCA and the corresponding insertion of
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Synthetic rescue couples NADPH generation to metabolite overproduction in
Saccharomyces cerevisiae
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Motter, Adilson E; Gulbahce, Natali; Almaas, Eivind; Barabási, Albert-László (2008).
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strong form of synthetic rescue has been recently observed in experiments in both
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partially completed polypeptide or degradation of the
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Synthetic rescue could potentially be exploited for
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307:, PLoS Genetics 14(3), e1007284 (2018).
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102:Genetic suppression can be mediated by
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68:Interaction-mediated suppression
39:(equivalent to a knockout) or a
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336:10.1016/j.celrep.2019.06.067
54:Types of genetic suppression
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59:Dosage-mediated suppression
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178:Molecular Systems Biology
98:tRNA-mediated suppression
64:of the initial mutation.
41:gain-of-function mutation
37:loss-of-function mutation
85:Saccharomyces cerevisiae
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121:nonsense-mediated decay
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143:Suppressor mutation
25:synthetic viability
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